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Invitrogen
HMGB1 Monoclonal Antibody (5H6)
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Product Details
MA5-49047
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Armenian hamster
/ IgG
Class
Monoclonal
Type
Antibody
Clone
5H6
Immunogen
HMGB1 (High Mobility Group Box-1 protein
Conjugate
Form
Liquid
Concentration
100 µg/mL
Purification
Size-exclusion - Dialysis
Storage buffer
PBS with 0.1% BSA
Contains
0.02% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_3091093
Product Specific Information
HMGB1 (High Mobility Group Box-1 protein also known as amphoterin) is a highly conserved protein with more than 95% amino acid identity between rodent and human HMGB1.
Target Information
HMGB1 (High-mobility group box-1) protein was originally described as a nuclear non-histone DNA binding chromosomal protein. However, recent studies indicate that damaged, necrotic cells liberate HMGB1 into the extracellular milieu where it functions as a proinflammatory cytokine. Mouse HMGB1 is expressed as a 215 amino acid single chain polypeptide containing three domains: two tandem-linked positively charged DNA-binding domains (HMG box A, aa 9-79; and box B, aa 89-162), and a negatively charged 30 aa C-terminal acidic tail region. Residues 28 - 44 and 180 - 185 contain a nuclear localization signal (NLS). The cytokine activity of HMGB1 is contained in the B box, while the A box is associated with the helix-loop-helix domain of transcription factors. HMGB1 acts both as an inflammatory mediator that promotes monocyte migration and cytokine secretion, as well as a mediator of T cell-dendritic cell interaction. HMGB1 is secreted and acts to transduce cellular signals through its high affinity receptor, RAGE and possibly, TLR2 and TLR4. HMGB1 is highly conserved and ubiquitous in the nuclei and cytoplasm of nearly all cell types, is a necessary and sufficient mediator of inflammation during sterile and infection-associated responses. HMGB1 also act as DNA nuclear binding protein that has recently been shown to be an early trigger of sterile inflammation in animal models of trauma-hemorrhage via the activation of the Toll-like receptor 4 (TLR4) and the receptor for the advanced glycation endproducts (RAGE). Moreover, HMGB1 is reported that the level of HMGB1 is elevated during sterile tissue injury, infection, lethal endotoxemia or sepsis, collagen-induced arthritis, and ischemia-reperfusion induced tissue injury.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Amphoterin antibody; DKFZp686A04236; High mobility group protein 1; High mobility group protein B1; HMG-1; HMG3 antibody; hmgb 1; hmgb-1; RP11-550P23.1
Gene Aliases: amphoterin; DEF; HMG-1; Hmg1; Hmgb1; p30; SBP-1
UniProt ID: (Mouse) P63158
Entrez Gene ID: (Mouse) 15289
eye development
positive regulation of protein phosphorylation
positive regulation of mesenchymal cell proliferation
chromatin remodeling
lung development
positive regulation of cell migration
DNA geometric change
positive regulation of myeloid cell differentiation
positive regulation of glycogen catabolic process
positive regulation of protein kinase activity
positive regulation of mitotic cell cycle
positive regulation of transcription from RNA polymerase II promoter
induction of positive chemotaxis
response to glucocorticoid
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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