Proteintech

HMGB2 Polyclonal Antibody

HMGB2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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HMGB2 Antibody (14597-1-AP) in IHC (P)

Immunohistochemical analysis of paraffin-embedded Mouse brain tissue slide using (Product # 14597-1-AP) HMGB2 antibody at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

HMGB2 Antibody in Immunoprecipitation (IP)

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Product Details

14597-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

View 10 publications 10 publications

Immunohistochemistry (IHC)

View 6 publications 6 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Immunoprecipitation (IP)

0.5-4.0 µg

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

HMGB2 Fusion Protein Ag6135 (43-207 aa encoded by BC001063)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.2 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

The calculated molecular weight of HMGB2 is 24 kDa, but the post-modification of HMGB2 is about 33-35 kDa.

Immunogen sequence: KKCSERWKT MSAKEKSKFE DMAKSDKARY DREMKNYVPP KGDKKGKKKD PNAPKRPPSA FFLFCSEHRP KIKSEHPGLS IGDTAKKLGE MWSEQSAKDK QPYEQKAAKL KEKYEKDIAA YRAKGKSEAG KKGPGRPTGS KKKNEPEDEE EEEEEEDEDE EEEDED (43-207 aa encoded by BC001063)

Target Information

HMGB2 encodes a member of the non-histone chromosomal high mobility group protein family. The proteins of this family are chromatin-associated and ubiquitously distributed in the nucleus of higher eukaryotic cells. In vitro studies have demonstrated that this protein is able to efficiently bend DNA and form DNA circles. These studies suggest a role in facilitating cooperative interactions between cis-acting proteins by promoting DNA flexibility. This protein was also reported to be involved in the final ligation step in DNA end-joining processes of DNA double-strand breaks repair and V(D)J recombination.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Platinum-Based Regimens Are Active in Advanced Pediatric-Type Rhabdomyosarcoma in Adults and Depending on HMGB1 Expression.

International journal of molecular sciences

Hindi N,Carrillo-García J,Blanco-Alcaina E,Renshaw M,Luna P,Durán J,Jiménez N,Sancho P,Ramos R,Moura DS,Martín-Broto J

Tue Jan 03 00:00:00 UTC 2023

Dissection of gastric homeostasis in vivo facilitates permanent capture of isthmus-like stem cells in vitro.

Nature cell biology

Huebner AJ,Gorelov RA,Deviatiiarov R,Demharter S,Kull T,Walsh RM,Taylor MS,Steiger S,Mullen JT,Kharchenko PV,Hochedlinger K

Wed Mar 01 00:00:00 UTC 2023

Inflachromene inhibits intimal hyperplasia through the HMGB1/2- regulated TLR4-NF-κB pathway.

International immunopharmacology

Teng S,Zhu Z,Wu C,He Y,Zhou S

Thu Jun 01 00:00:00 UTC 2023

Inflachromene inhibits intimal hyperplasia through the HMGB1/2- regulated TLR4-NF-κB pathway.

International immunopharmacology

Teng S,Zhu Z,Wu C,He Y,Zhou S

Thu Jun 01 00:00:00 UTC 2023

Inflachromene inhibits intimal hyperplasia through the HMGB1/2- regulated TLR4-NF-κB pathway.

International immunopharmacology

Teng S,Zhu Z,Wu C,He Y,Zhou S

Thu Jun 01 00:00:00 UTC 2023

Inflachromene inhibits intimal hyperplasia through the HMGB1/2- regulated TLR4-NF-κB pathway.

International immunopharmacology

Teng S,Zhu Z,Wu C,He Y,Zhou S

Thu Jun 01 00:00:00 UTC 2023

Proteomic profiling of concurrently isolated primary microvascular endothelial cells, pericytes, and vascular smooth muscle cells from adult mouse heart.

Scientific reports

Cao Z,Minnier J,Liu L,Scott KLL,Reddy AP,Wilmarth PA,David LL,Barnes AP,Grafe MR,Kaul S,Alkayed NJ,Davis CM

Wed May 25 00:00:00 UTC 2022

LncRNA SNHG16 accelerates atherosclerosis and promotes ox-LDL-induced VSMC growth via the miRNA-22-3p/HMGB2 axis.

European journal of pharmacology

Wang Y,Yang Y,Zhang T,Jia S,Ma X,Zhang M,Wang L,Ma A

Sat Jan 15 00:00:00 UTC 2022

The LncRNA RP11-301G19.1/miR-582-5p/HMGB2 axis modulates the proliferation and apoptosis of multiple myeloma cancer cells via the PI3K/AKT signalling pathway.

Cancer gene therapy

Wang F,Luo Y,Zhang L,Younis M,Yuan L

Tue Mar 01 00:00:00 UTC 2022

Nuclear exosome HMGB3 secreted by nasopharyngeal carcinoma cells promotes tumour metastasis by inducing angiogenesis.

Cell death & disease

Zhang K,Liu D,Zhao J,Shi S,He X,Da P,You Y,You B

Fri May 28 00:00:00 UTC 2021

Arsenic influences spermatogenesis by disorganizing the elongation of spermatids in adult male mice.

Chemosphere

Han Y,Liang C,Manthari RK,Yu Y,Gao Y,Liu Y,Jiang S,Tikka C,Wang J,Zhang J

Wed Jan 01 00:00:00 UTC 2020

HMGB2 promotes the malignancy of human gastric cancer and indicates poor survival outcome.

Human pathology

Cui G,Cai F,Ding Z,Gao L

Fri Feb 01 00:00:00 UTC 2019

HMGB2 promotes the malignancy of human gastric cancer and indicates poor survival outcome.

Human pathology

Cui G,Cai F,Ding Z,Gao L

Fri Feb 01 00:00:00 UTC 2019

HMGB2 promotes the malignancy of human gastric cancer and indicates poor survival outcome.

Human pathology

Cui G,Cai F,Ding Z,Gao L

Fri Feb 01 00:00:00 UTC 2019

HMGB2 promotes the malignancy of human gastric cancer and indicates poor survival outcome.

Human pathology

Cui G,Cai F,Ding Z,Gao L

Fri Feb 01 00:00:00 UTC 2019

Fluoride-induced unrestored arrest during haploid period of spermatogenesis via the regulation of DDX25 in rats.

Environmental pollution (Barking, Essex : 1987)

Han Y,Yu Y,Liang C,Shi Y,Zhu Y,Zheng H,Wang J,Zhang J

Tue Oct 01 00:00:00 UTC 2019

HMGB2 is associated with malignancy and regulates Warburg effect by targeting LDHB and FBP1 in breast cancer.

Cell communication and signaling : CCS

Fu D,Li J,Wei J,Zhang Z,Luo Y,Tan H,Ren C

Tue Feb 20 00:00:00 UTC 2018

HMGB2 is associated with malignancy and regulates Warburg effect by targeting LDHB and FBP1 in breast cancer.

Cell communication and signaling : CCS

Fu D,Li J,Wei J,Zhang Z,Luo Y,Tan H,Ren C

Tue Feb 20 00:00:00 UTC 2018

HMGB2 is associated with malignancy and regulates Warburg effect by targeting LDHB and FBP1 in breast cancer.

Cell communication and signaling : CCS

Fu D,Li J,Wei J,Zhang Z,Luo Y,Tan H,Ren C

Tue Feb 20 00:00:00 UTC 2018

HMGB2 is associated with malignancy and regulates Warburg effect by targeting LDHB and FBP1 in breast cancer.

Cell communication and signaling : CCS

Fu D,Li J,Wei J,Zhang Z,Luo Y,Tan H,Ren C

Tue Feb 20 00:00:00 UTC 2018

Expression of HMGB2 indicates worse survival of patients and is required for the maintenance of Warburg effect in pancreatic cancer.

Acta biochimica et biophysica Sinica

Cai X,Ding H,Liu Y,Pan G,Li Q,Yang Z,Liu W

Mon Feb 06 00:00:00 UTC 2017

Expression of HMGB2 indicates worse survival of patients and is required for the maintenance of Warburg effect in pancreatic cancer.

Acta biochimica et biophysica Sinica

Cai X,Ding H,Liu Y,Pan G,Li Q,Yang Z,Liu W

Mon Feb 06 00:00:00 UTC 2017

Expression of HMGB2 indicates worse survival of patients and is required for the maintenance of Warburg effect in pancreatic cancer.

Acta biochimica et biophysica Sinica

Cai X,Ding H,Liu Y,Pan G,Li Q,Yang Z,Liu W

Mon Feb 06 00:00:00 UTC 2017

Expression of HMGB2 indicates worse survival of patients and is required for the maintenance of Warburg effect in pancreatic cancer.

Acta biochimica et biophysica Sinica

Cai X,Ding H,Liu Y,Pan G,Li Q,Yang Z,Liu W

Mon Feb 06 00:00:00 UTC 2017

Members of the high mobility group B protein family are dynamically expressed in embryonic neural stem cells.

Proteome science

Abraham AB,Bronstein R,Chen EI,Koller A,Ronfani L,Maletic-Savatic M,Tsirka SE

Sat Apr 27 00:00:00 UTC 2013

Members of the high mobility group B protein family are dynamically expressed in embryonic neural stem cells.

Proteome science

Abraham AB,Bronstein R,Chen EI,Koller A,Ronfani L,Maletic-Savatic M,Tsirka SE

Sat Apr 27 00:00:00 UTC 2013

Bioinformatics

Protein Aliases: High mobility group protein 2; High mobility group protein B2; high-mobility group (nonhistone chromosomal) protein 2; high-mobility group box 2; HMG 2; HMG B2; HMG-2; HMG-B2; HMGB-2

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Gene Aliases: C80539; HMG-2; HMG2; HMGB2

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UniProt ID: (Human) P26583, (Rat) P52925, (Mouse) P30681

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Entrez Gene ID: (Human) 3148, (Rat) 29395, (Mouse) 97165

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Function(s)

four-way junction DNA binding enhancer sequence-specific DNA binding DNA binding damaged DNA binding double-stranded DNA binding single-stranded DNA binding transcription factor activity, sequence-specific DNA binding protein binding transcription factor binding drug binding DNA binding, bending protein domain specific binding chemoattractant activity transcription regulatory region DNA binding non-sequence-specific DNA binding, bending poly(A) RNA binding RAGE receptor binding supercoiled DNA binding lipid binding nucleosomal histone binding chromatin binding

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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HMGB2 Polyclonal Antibody

HMGB2 Antibody (14597-1-AP) in IHC (P)

Product Details

14597-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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