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HMGN2 Antibody (PA5-95559) in IHC (P)
Immunohistochemistry analysis of HMGN2 in paraffin-embedded human esophagus squama cancer tissue. Antigen retrieval was performed on the tissue using citrate buffer (pH 6, 20 min) and blocked with 10% goat serum. Samples were incubated with HMGN2 polyclonal antibody (Product # PA5-95559) at a 2 µg/mL dilution, followed by biotinylated goat anti-rabbit IgG (30 min, 37°C), and developed with Strepavidin-Bioti... View More
Product Details
PA5-95559
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to a sequence of human HMGN2 (KKGKADAGKEGNNPAENGDAKTDQAQKAEGAGDAK).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2807361
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: human SH-SY5Y whole cell, human HL-60 whole cell. IHC: human rectal cancer tissue, human tonsil tissue, human thyroid cancer tissue, human liver cancer tissue. ICC/IF: MCF7 cell. Flow: Raji cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Target Information
The protein encoded by this gene binds nucleosomal DNA and is associated with transcriptionally active chromatin. Along with a similar protein, HMGN1, the encoded protein may help maintain an open chromatin configuration around transcribable genes. The protein has also been found to have antimicrobial activity against bacteria, viruses and fungi.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: High mobility group nucleosome-binding domain-containing protein 2; high mobility group protein N2; high-mobility group (nonhistone chromosomal) protein 17; high-mobility group nucleosomal binding domain 2; Non-histone chromosomal protein HMG-17; nonhistone chromosomal protein HMG-17
Gene Aliases: HMG17; HMGN2
UniProt ID: (Human) P05204
Entrez Gene ID: (Human) 3151
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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