Invitrogen

HMOX1 Monoclonal Antibody (HO-1-1)

HMOX1 Antibody in Immunocytochemistry (ICC/IF)

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HMOX1 Antibody (MA1-112) in ICC/IF

Immunofluorescent analysis of Heme Oxygenase-1 (HO-1, green) in HeLa cells either left untreated (left panel) or treated with 0.5mM copper sulfate (right panel) for 24 hours. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA (Product # 37525) for 15 minutes at room temperature. Cells were probed with an HO-1 monoclonal antibody (Product # MA1-112) at a dilution of 1:100 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight ... View More

HMOX1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

HMOX1 Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)

HMOX1 Antibody in Immunohistochemistry (IHC)

HMOX1 Antibody in Western Blot, Immunohistochemistry (WB, IHC)

Product Image Gallery

HMOX1 Antibody — Modulation of target protein expression by cell treatment demonstrates antibody specificity. Immunofluorescence analysis of HO-1 monoclonal antibody (Product # MA1-112) shows induced expression of HO-1 in the HeLa cells upon treatment with copper sulphate. {TM}

Product Details

MA1-112

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 17 publications 17 publications

Immunohistochemistry (IHC)

View 5 publications 5 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000

View 1 publication 1 publication

Immunohistochemistry (PFA fixed) (IHC (PFA))

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:50-1:200

View 4 publications 4 publications

Flow Cytometry (Flow)

10 µg/mL

Product Specifications

Species Reactivity

Bovine, Dog, Human, Mouse, Rat

Published species

Guinea pig, Human, Mouse, Rat

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

HO-1-1

Immunogen

Synthetic peptide corresponding to the sequence near the N-terminus (amino acids 1-30) of human Heme Oxygenase 1

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS with 50% glycerol

Contains

0.09% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2536823

Product Specific Information

This antibody detects an ~32 kDa protein corresponding to the apparent molecular mass of HO-1 on SDS-PAGE immunoblots, in samples from human, mouse, rat, bovine and dog origins. This antibody does not cross-react with HO-2.

This antibody was originally validated as part of a Thermo Scientific Cellomics High Content Screening Kit. The antibody sold separately may have slightly different performance and may need to be further optimized for the best results.

Target Information

Heme oxygenase (HO) is a microsomal enzyme that catalyzes the oxidation of heme to the antioxidant molecules, biliverdin and carbon monoxide. HO consists of two homologous isozymes, an inducible HO-1 and a constitutively expressed HO-2. HO-1 is induced by a wide variety of stimuli including conditions of oxidative stress, inflammatory agents, transforming growth factor beta and heat shock. The increase in expression of HO-1 is thought to be a cellular defense mechanism against oxidative stress since elevated HO could eventually generate more bilirubin, an anti-oxidant.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Design and characterization of a heterobifunctional degrader of KEAP1.

Redox biology

Chen H,Nguyen NH,Magtoto CM,Cobbold SA,Bidgood GM,Meza Guzman LG,Richardson LW,Corbin J,Au AE,Lechtenberg BC,Feltham R,Sutherland KD,Grohmann C,Nicholson SE,Sleebs BE

MA1-112 was used in Western Blotting to develop a proteolysis targeting chimera (PROTAC) that depletes KEAP1 from cells through the ubiquitin-proteasome pathway.

Wed Feb 01 00:00:00 UTC 2023

Design and characterization of a heterobifunctional degrader of KEAP1.

Redox biology

Chen H,Nguyen NH,Magtoto CM,Cobbold SA,Bidgood GM,Meza Guzman LG,Richardson LW,Corbin J,Au AE,Lechtenberg BC,Feltham R,Sutherland KD,Grohmann C,Nicholson SE,Sleebs BE

MA1-112 was used in Western Blotting to develop a proteolysis targeting chimera (PROTAC) that depletes KEAP1 from cells through the ubiquitin-proteasome pathway.

Wed Feb 01 00:00:00 UTC 2023

Evaluation of the Involvement of Heme Oxygenase-1 Expression in Discoid Lupus Erythematosus Lesions.

Antioxidants (Basel, Switzerland)

Fagone P,Piombino E,Mangano K,De Pasquale R,Nicoletti F,Caltabiano R

MA1-112 was used in Immunohistochemistry to investigate the potential clinical and therapeutic applications of heme oxygenase-1 (HMOX1) in the context of discoid lupus erythematosus (DLE).

Tue Jun 27 00:00:00 UTC 2023

Evaluation of the Involvement of Heme Oxygenase-1 Expression in Discoid Lupus Erythematosus Lesions.

Antioxidants (Basel, Switzerland)

Fagone P,Piombino E,Mangano K,De Pasquale R,Nicoletti F,Caltabiano R

MA1-112 was used in Immunohistochemistry to investigate the potential clinical and therapeutic applications of heme oxygenase-1 (HMOX1) in the context of discoid lupus erythematosus (DLE).

Tue Jun 27 00:00:00 UTC 2023

Polymerized human cord hemoglobin assisted with ascorbic acid as a red blood cell substitute alleviating oxidative stress for blood transfusion.

Frontiers in bioengineering and biotechnology

Kong W,Zhou W,He Z,Zhang X,Li S,Zhong R,Liu J

MA1-112 was used in Western Blot to demonstrate that kidney tissue damage was effectively alleviated.

Tue Mar 14 00:00:00 UTC 2023

Polymerized human cord hemoglobin assisted with ascorbic acid as a red blood cell substitute alleviating oxidative stress for blood transfusion.

Frontiers in bioengineering and biotechnology

Kong W,Zhou W,He Z,Zhang X,Li S,Zhong R,Liu J

MA1-112 was used in Western Blot to demonstrate that kidney tissue damage was effectively alleviated.

Tue Mar 14 00:00:00 UTC 2023

Kynurenine/Aryl Hydrocarbon Receptor Modulates Mitochondria-Mediated Oxidative Stress and Neuronal Apoptosis in Experimental Intracerebral Hemorrhage.

Antioxidants & redox signaling

Ren R,Fang Y,Sherchan P,Lu Q,Lenahan C,Zhang JH,Zhang J,Tang J

Published figure using HMOX1 monoclonal antibody (Product # MA1-112) in Western Blot

Thu Dec 01 00:00:00 UTC 2022

Human iPSC-derived renal organoids engineered to report oxidative stress can predict drug-induced toxicity.

iScience

Lawrence ML,Elhendawi M,Morlock M,Liu W,Liu S,Palakkan A,Seidl LF,Hohenstein P,Sjögren AK,Davies JA

MA1-112 was used in Immunocytochemistry, Immunohistochemistry (PFA fixed) to highlight the potential usefulness, and some limitations, of HMOX1-reporter renal organoids as screening tools.

Fri Mar 18 00:00:00 UTC 2022

Human iPSC-derived renal organoids engineered to report oxidative stress can predict drug-induced toxicity.

iScience

Lawrence ML,Elhendawi M,Morlock M,Liu W,Liu S,Palakkan A,Seidl LF,Hohenstein P,Sjögren AK,Davies JA

MA1-112 was used in Immunocytochemistry, Immunohistochemistry (PFA fixed) to highlight the potential usefulness, and some limitations, of HMOX1-reporter renal organoids as screening tools.

Fri Mar 18 00:00:00 UTC 2022

Novel Secreted Peptides From Rhizopus arrhizus var. delemar With Immunomodulatory Effects That Enhance Fungal Pathogenesis.

Frontiers in microbiology

Soliman SSM,El-Labbad EM,Abu-Qiyas A,Fayed B,Hamoda AM,Al-Rawi AM,Dakalbab S,El-Shorbagi AA,Hamad M,Ibrahim AS,Mohammad MG

Published figure using HMOX1 monoclonal antibody (Product # MA1-112) in Western Blot

Sat May 13 00:00:00 UTC 2023

Enhanced cytotoxicity of a novel family of ATPase inhibitors in colorectal cancer cells with high NAT2 activity.

Biochemical pharmacology

Zhang X,Akcan E,Correia M,Rameika N,Kundu S,Stoimenov I,Rendo V,Eriksson AU,Haraldsson M,Globisch D,Sjöblom T

MA1-112 was used in Western Blotting to report a novel compound CBK034026C that exhibits specific toxicity towards CRC cells with high NAT2 activity.

Thu Sep 01 00:00:00 UTC 2022

Analysis of immune-related key genes in Alzheimer's disease.

Bioengineered

Wu Y,Liang S,Zhu H,Zhu Y

MA1-112 was used in Western Blotting to reveal that 15 modules were obtained through weighted gene co-expression network analysis, among which the magenta and blue modules were significantly associated with Alzheimer's Disease (AD).

Wed Dec 01 00:00:00 UTC 2021

Esomeprazole attenuates inflammatory and fibrotic response in lung cells through the MAPK/Nrf2/HO1 pathway.

Journal of inflammation (London, England)

Ebrahimpour A,Wang M,Li L,Jegga AG,Bonnen MD,Eissa NT,Raghu G,Jyothula S,Kheradmand F,Hanania NA,Rosas IO,Ghebre YT

MA1-112 was used in Immunocytochemistry to investigate molecular mechanism(s) by which proton pump inhibitors favorably regulate the process of idiopathic pulmonary fibrosis.

Wed May 19 00:00:00 UTC 2021

Heme Oxygenase-1 Contributes to Both the Engulfment and the Anti-Inflammatory Program of Macrophages during Efferocytosis.

Cells

Fige É,Szendrei J,Sós L,Kraszewska I,Potor L,Balla J,Szondy Z

Published figure using HMOX1 monoclonal antibody (Product # MA1-112) in Western Blot

Mon Mar 15 00:00:00 UTC 2021

Glial Nrf2 signaling mediates the neuroprotection exerted by Gastrodia elata Blume in Lrrk2-G2019S Parkinson's disease.

eLife

Lin YE,Lin CH,Ho EP,Ke YC,Petridi S,Elliott CJ,Sheen LY,Chien CT

Published figure using HMOX1 monoclonal antibody (Product # MA1-112) in Western Blot

Mon Nov 15 00:00:00 UTC 2021

HO-1 overexpression alleviates senescence by inducing autophagy via the mitochondrial route in human nucleus pulposus cells.

Journal of cellular physiology

Yi W,Lan H,Wen Y,Wang Y,He D,Bai Z,Zhang Y,Jiang W,Liu B,Shen J,Hu Z

MA1-112 was used in Immunocytochemistry, Immunohistochemistry, and Western Blot to study the role of heme oxygenase-1 (HO-1) in nucleus pulposus (NP) cell senescence and autophagy in intervertebral disc degeneration (IDD).

Sun Nov 01 00:00:00 UTC 2020

HO-1 overexpression alleviates senescence by inducing autophagy via the mitochondrial route in human nucleus pulposus cells.

Journal of cellular physiology

Yi W,Lan H,Wen Y,Wang Y,He D,Bai Z,Zhang Y,Jiang W,Liu B,Shen J,Hu Z

Sun Nov 01 00:00:00 UTC 2020

HO-1 overexpression alleviates senescence by inducing autophagy via the mitochondrial route in human nucleus pulposus cells.

Journal of cellular physiology

Yi W,Lan H,Wen Y,Wang Y,He D,Bai Z,Zhang Y,Jiang W,Liu B,Shen J,Hu Z

Sun Nov 01 00:00:00 UTC 2020

HO-1 overexpression alleviates senescence by inducing autophagy via the mitochondrial route in human nucleus pulposus cells.

Journal of cellular physiology

Yi W,Lan H,Wen Y,Wang Y,He D,Bai Z,Zhang Y,Jiang W,Liu B,Shen J,Hu Z

Sun Nov 01 00:00:00 UTC 2020

HO-1 overexpression alleviates senescence by inducing autophagy via the mitochondrial route in human nucleus pulposus cells.

Journal of cellular physiology

Yi W,Lan H,Wen Y,Wang Y,He D,Bai Z,Zhang Y,Jiang W,Liu B,Shen J,Hu Z

Sun Nov 01 00:00:00 UTC 2020

Biochemical study on the protective effect of curcumin on acetaminophen and gamma-irradiation induced hepatic toxicity in rats.

Environmental toxicology

Eassawy MMT,Salem AA,Ismail AFM

Published figure using HMOX1 monoclonal antibody (Product # MA1-112) in Western Blot

Thu Dec 17 00:00:00 UTC 2020

Discovery of Mitochondrial Transcription Inhibitors Active in Pancreatic Cancer Cells.

ChemMedChem

Chen W,Hu S,Mao S,Xu Y,Guo H,Li H,Paulsen MT,Chen X,Ljungman M,Neamati N

Published figure using HMOX1 monoclonal antibody (Product # MA1-112) in Western Blot

Wed Nov 04 00:00:00 UTC 2020

NRF2 and PPAR-γ Pathways in Oligodendrocyte Progenitors: Focus on ROS Protection, Mitochondrial Biogenesis and Promotion of Cell Differentiation.

International journal of molecular sciences

De Nuccio C,Bernardo A,Troiano C,Brignone MS,Falchi M,Greco A,Rosini M,Basagni F,Lanni C,Serafini MM,Minghetti L,Visentin S

MA1-112 was used in Western Blot, Immunocytochemistry to show NRF2 and PPAR-γ have complementary functions aimed at the preservation of mitochondrial function, the defense against oxidative stress and the promotion of OP differentiation.

Tue Sep 29 00:00:00 UTC 2020

NRF2 and PPAR-γ Pathways in Oligodendrocyte Progenitors: Focus on ROS Protection, Mitochondrial Biogenesis and Promotion of Cell Differentiation.

International journal of molecular sciences

De Nuccio C,Bernardo A,Troiano C,Brignone MS,Falchi M,Greco A,Rosini M,Basagni F,Lanni C,Serafini MM,Minghetti L,Visentin S

Tue Sep 29 00:00:00 UTC 2020

NRF2 and PPAR-γ Pathways in Oligodendrocyte Progenitors: Focus on ROS Protection, Mitochondrial Biogenesis and Promotion of Cell Differentiation.

International journal of molecular sciences

De Nuccio C,Bernardo A,Troiano C,Brignone MS,Falchi M,Greco A,Rosini M,Basagni F,Lanni C,Serafini MM,Minghetti L,Visentin S

Tue Sep 29 00:00:00 UTC 2020

NRF2 and PPAR-γ Pathways in Oligodendrocyte Progenitors: Focus on ROS Protection, Mitochondrial Biogenesis and Promotion of Cell Differentiation.

International journal of molecular sciences

De Nuccio C,Bernardo A,Troiano C,Brignone MS,Falchi M,Greco A,Rosini M,Basagni F,Lanni C,Serafini MM,Minghetti L,Visentin S

Tue Sep 29 00:00:00 UTC 2020

High-throughput small molecule screening reveals Nrf2-dependent and -independent pathways of cellular stress resistance.

Science advances

Lombard DB,Kohler WJ,Guo AH,Gendron C,Han M,Ding W,Lyu Y,Ching TT,Wang FY,Chakraborty TS,Nikolovska-Coleska Z,Duan Y,Girke T,Hsu AL,Pletcher SD,Miller RA

MA1-112 was used in Western Blotting to identify compounds that induced resistance to the superoxide generator paraquat (PQ), the heavy metal cadmium (Cd), and the DNA alkylator methyl methanesulfonate (MMS).

Thu Oct 01 00:00:00 UTC 2020

High-throughput small molecule screening reveals Nrf2-dependent and -independent pathways of cellular stress resistance.

Science advances

Lombard DB,Kohler WJ,Guo AH,Gendron C,Han M,Ding W,Lyu Y,Ching TT,Wang FY,Chakraborty TS,Nikolovska-Coleska Z,Duan Y,Girke T,Hsu AL,Pletcher SD,Miller RA

Thu Oct 01 00:00:00 UTC 2020

BACH1 Stabilization by Antioxidants Stimulates Lung Cancer Metastasis.

Cell

Wiel C,Le Gal K,Ibrahim MX,Jahangir CA,Kashif M,Yao H,Ziegler DV,Xu X,Ghosh T,Mondal T,Kanduri C,Lindahl P,Sayin VI,Bergo MO

MA1-112 was used in Western Blotting to show that long-term supplementation with the antioxidants N-acetylcysteine and vitamin E promotes KRAS-driven lung cancer metastasis.

Thu Jul 11 00:00:00 UTC 2019

The immunophenotype of decidual macrophages in acute atherosis.

American journal of reproductive immunology (New York, N.Y. : 1989)

Gill N,Leng Y,Romero R,Xu Y,Panaitescu B,Miller D,Arif A,Mumuni S,Qureshi F,Hsu CD,Hassan SS,Staff AC,Gomez-Lopez N

MA1-112 was used in Immunohistochemistry to provide a molecular foundation for future mechanistic inquiries about the role of pro-inflammatory macrophages in the pathogenesis of acute atherosis.

Mon Apr 01 00:00:00 UTC 2019

Role of OSGIN1 in mediating smoking-induced autophagy in the human airway epithelium.

Autophagy

Wang G,Zhou H,Strulovici-Barel Y,Al-Hijji M,Ou X,Salit J,Walters MS,Staudt MR,Kaner RJ,Crystal RG

MA1-112 was used in Immunohistochemistry to identify smoking upregulated genes known to respond to oxidative stress and the DNA damage response, via microarray analysis of the airway epithelium.

Mon Jul 03 00:00:00 UTC 2017

Activation of PPARγ by baicalin attenuates pulmonary hypertension in an infant rat model by suppressing HMGB1/RAGE signaling.

FEBS open bio

Chen Z,Wang Q

MA1112 was used in western blot to elucidate the effects of baicalin in an infant rodent model of pulmonary hypertension

Sat Apr 01 00:00:00 UTC 2017

Activation of PPARγ by baicalin attenuates pulmonary hypertension in an infant rat model by suppressing HMGB1/RAGE signaling.

FEBS open bio

Chen Z,Wang Q

MA1112 was used in western blot to elucidate the effects of baicalin in an infant rodent model of pulmonary hypertension

Sat Apr 01 00:00:00 UTC 2017

Real-time in vivo imaging reveals localised Nrf2 stress responses associated with direct and metabolism-dependent drug toxicity.

Scientific reports

Forootan SS,Mutter FE,Kipar A,Iwawaki T,Francis B,Goldring CE,Park BK,Copple IM

Published figure using HMOX1 monoclonal antibody (Product # MA1-112) in Immunohistochemistry

Wed Nov 22 00:00:00 UTC 2017

Anti-fatigue effect of Myelophil in a chronic forced exercise mouse model.

European journal of pharmacology

Lee JS,Kim HG,Han JM,Kim YA,Son CG

MA1-112 was used in western blot to assess the anti-fatigue effects of Myelophil.

Mon Oct 05 00:00:00 UTC 2015

The traditional drug Gongjin-Dan ameliorates chronic fatigue in a forced-stress mouse exercise model.

Journal of ethnopharmacology

Hong SS,Lee JY,Lee JS,Lee HW,Kim HG,Lee SK,Park BK,Son CG

MA1-112 was used in western blot to evaluate the anti-fatigue effects of Gongjin-Dan in a chronic forced exercise mouse model

Sat Jun 20 00:00:00 UTC 2015

Bioinformatics

Protein Aliases: heat shock protein, 32-kD; heme orygenase 1; heme oxygenase (decycling) 1; heme oxygenase (decyclizing) 1; Heme oxygenase 1; hemoxygenase; HO-1; HSP32; P32 protein

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Gene Aliases: bK286B10; BOS_5643; D8Wsu38e; Hemox; Heox; HEOXG; Hmox; HMOX1; HMOX1D; HO; HO-1; HO1; HSP32

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UniProt ID: (Human) P09601, (Bovine) Q5E9F2, (Rat) P06762, (Mouse) P14901

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Entrez Gene ID: (Human) 3162, (Dog) 442987, (Bovine) 513221, (Rat) 24451, (Mouse) 15368

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Function(s)

heme oxygenase (decyclizing) activity phospholipase D activity signal transducer activity protein binding enzyme binding heme binding protein homodimerization activity metal ion binding oxidoreductase activity

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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HMOX1 Monoclonal Antibody (HO-1-1)

HMOX1 Antibody (MA1-112) in ICC/IF

Product Details

MA1-112

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (PFA fixed) (IHC (PFA))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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