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HNF1-alpha Antibody (A304-053A) in IHC (P)
Detection of human HNF1-alpha by immunohistochemistry. Samples: FFPE serial sections of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-HNF1-alpha (Product # A304-051A) (Lot 1), (Product # A304-052A) (Lot 1), (Product # A304-053A) (Lot 1), and (Product # A304-054A) (Lot 1) used at a dilution of 1:5,000 (0.2 µg/mL). Detection: DAB.
Product Details
A304-053A
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Region between residue 550 to 600 of human HNF1 Homeobox A.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
phosphate/tris citrate, pH 7-8
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
The recommended shelf life for this product is 1 year from date of receipt.
Application Note: For IHC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Target Information
This gene is expressed ubiquitously with higher levels in fetal than in adult tissues. It encodes a protein sharing 93% sequence identity with the mouse protein. Wolf-Hirschhorn syndrome (WHS) is a malformation syndrome associated with a hemizygous deletion of the distal short arm of chromosome 4. This gene is mapped to the 165 kb WHS critical region, and may play a role in the phenotype of the WHS or Pitt-Rogers-Danks syndrome. The encoded protein is found to be capable of reacting with HLA-A2-restricted and tumor-specific cytotoxic T lymphocytes, suggesting a target for use in specific immunotherapy for a large number of cancer patients. This protein has also been shown to be a member of the NELF (negative elongation factor) protein complex that participates in the regulation of RNA polymerase II transcription elongation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: albumin proximal factor; hepatic nuclear factor 1; Hepatocyte nuclear factor 1-alpha; hnf-1 alpha; HNF-1-alpha; interferon production regulator factor; LFB1; Liver-specific transcription factor LF-B1; TCF-1; Transcription factor 1; transcription factor 1, hepatic
Gene Aliases: HNF-1A; HNF1; HNF1A; IDDM20; LFB1; MODY3; TCF-1; TCF1
UniProt ID: (Human) P20823
Entrez Gene ID: (Human) 6927
RNA polymerase II regulatory region sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
transcription factor activity, sequence-specific DNA binding
protein binding
transcription regulatory region DNA binding
protein dimerization activity
regulation of transcription from RNA polymerase II promoter
transcription from RNA polymerase II promoter
regulation of pronephros size
renal glucose absorption
glucose homeostasis
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
glucose import
positive regulation of transcription initiation from RNA polymerase II promoter
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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