Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Product Details
H00003172-M04J
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG2a, kappa
Class
Monoclonal
Type
Antibody
Clone
400
Immunogen
HNF4A (NP_000448.3, 324 a.a. approximately 423 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Conjugate
Form
Liquid
Concentration
0.38 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.4
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
Product Specific Information
Product may be used with Western Blot (Cell lysate).
Immunogen sequence: NDRQYDSRGR FGELLLLLPT LQSITWQMIE QIQFIKLFGM AKIDNLLQEM LLGGSPSDAP HAHHPLHPHL MQEHMGTNVI VANTMPTHLS NGQMCEWPRP
Target Information
Hepatocyte nuclear factor 4 alpha (HNF4 alpha) is an orphan nuclear receptor. HNF4a is expressed in the liver, kidney, intestine, and pancreas. Mutation of HNF4a in humans has been associated with maturity-onset diabetes of the young type 1 (MODY1). HNF4 binds to DNA as an exclusive homodimer. The HNF4a gene is alternatively spliced and may generate up to nine different isoforms, HNF4a1 through HNF4a9.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: FLJ39654; hepatic nuclear factor 4 alpha; hepatocyte nuclear factor 4, alpha; Hepatocyte nuclear factor 4-alpha; HNF-4-alpha; HNF4-alpha; HNF4alpha10/11/12; Nuclear receptor subfamily 2 group A member 1; TCF-14; Transcription factor 14; Transcription factor HNF-4
Gene Aliases: FRTS4; HNF4; HNF4A; HNF4a7; HNF4a8; HNF4a9; HNF4alpha; MODY; MODY1; NR2A1; NR2A21; TCF; TCF14
UniProt ID: (Human) P41235
Entrez Gene ID: (Human) 3172
fatty-acyl-CoA binding
RNA polymerase II core promoter sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
RNA polymerase II activating transcription factor binding
DNA binding
transcription factor activity, sequence-specific DNA binding
transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding
steroid hormone receptor activity
RNA polymerase II transcription factor activity, ligand-activated sequence-specific DNA binding
receptor binding
fatty acid binding
protein binding
zinc ion binding
palmitoyl-CoA hydrolase activity
protein homodimerization activity
transcription regulatory region DNA binding
regulation of transcription from RNA polymerase II promoter
transcription initiation from RNA polymerase II promoter
ornithine metabolic process
lipid metabolic process
acyl-CoA metabolic process
xenobiotic metabolic process
establishment of tissue polarity
sex differentiation
blood coagulation
negative regulation of cell proliferation
response to glucose
regulation of gastrulation
regulation of lipid metabolic process
signal transduction involved in regulation of gene expression
cell differentiation
negative regulation of cell growth
intracellular receptor signaling pathway
regulation of microvillus assembly
negative regulation of protein import into nucleus, translocation
response to drug
negative regulation of tyrosine phosphorylation of Stat5 protein
glucose homeostasis
steroid hormone mediated signaling pathway
negative regulation of sequence-specific DNA binding transcription factor activity
cell-cell junction organization
positive regulation of gluconeogenesis
positive regulation of fatty acid biosynthetic process
positive regulation of transcription, DNA-templated
negative regulation of mitotic cell cycle
positive regulation of transcription from RNA polymerase II promoter
regulation of insulin secretion
response to cAMP
lipid homeostasis
phospholipid homeostasis
SMAD protein signal transduction
triglyceride homeostasis
negative regulation of activation of JAK2 kinase activity
positive regulation of cholesterol homeostasis
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support