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HNF6 Antibody (PA5-88749) in ICC/IF
Immunofluorescence analysis of ONECUT1 was performed using 70% confluent log phase Hep G2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with HNF6 Polyclonal Antibody (Product # PA5-88749) at 1:100 in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32790), at 1:... View More
Product Details
PA5-88749
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 167-292 of human ONECUT1 (NP_0044891)
Conjugate
Form
Liquid
Concentration
0.20 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.05% ProClin 300
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2805102
Product Specific Information
Immunogen sequence: PYHKDVAGMG QSLSPLSSSG LGSIHNSQQG LPHYAHPGAA MPTDKMLTPN GFEAHHPAML GRHGEQHLTP TSAGMVPING LPPHHPHAHL NAQGHGQLLG TAREPNPSVT GAQVSNGSNS GQMEEI; Positive Samples: Jurkat, Mouse brain, Mouse spleen, Mouse testis, Rat brain; Cellular Location: Nucleus
Target Information
Hepatocyte nuclear factor 6 is a CUT homeobox family protein which is a transcription factor and contains a CUT DNA-binding domain and a homeobox DNA-binding domain. It is an upstream regulator of several genes involved in the pathogenesis of maturity-onset diabetes of the young. HNF6 is a ligand of the FOXM1 transcription factor network. HNF6, along with HNF4, controls gene expression in the liver. The expression levels of HNF6 and HNF4 rise steadily during development and that up- or down regulation of HNF6 and HNF4 expression generate premature or delayed expression of the g6pc gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: hepatic nuclear factor 6; Hepatocyte nuclear factor 6; hepatocyte nuclear factor 6, alpha; HNF-3/forkhead homolog 12; HNF-6; One cut domain family member 1; one cut domain, family member 1; One cut homeobox 1
Gene Aliases: D9Ertd423e; Hfh12; HNF-6; HNF6; HNF6A; Hnf6b; OC-1; Oc1; ONECUT1
UniProt ID: (Human) Q9UBC0, (Mouse) O08755, (Rat) P70512
Entrez Gene ID: (Human) 3175, (Mouse) 15379, (Rat) 25231
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
DNA binding
transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
transcription factor activity, sequence-specific DNA binding
transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding
liver development
regulation of cell-matrix adhesion
epithelial cell development
glucose metabolic process
regulation of transcription, DNA-templated
transcription from RNA polymerase II promoter
Notch signaling pathway
endoderm development
cell differentiation
B cell differentiation
positive regulation of cell migration
negative regulation of transforming growth factor beta receptor signaling pathway
endocrine pancreas development
cilium assembly
cell fate commitment
positive regulation of transcription from RNA polymerase II promoter
spleen development
system development
transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
anatomical structure morphogenesis
pancreas development
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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