Invitrogen

HO-1 Monoclonal Antibody (1F12-A6), PerCP

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Datasheet
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Datasheet
Protocols
Questions & Answers

Product Details

MA5-45154

Applications
Tested Dilution
Publications

Western Blot (WB)

1:1,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:100
-

Immunocytochemistry (ICC/IF)

1:100
-

ELISA (ELISA)

Assay-dependent
-

Immunoprecipitation (IP)

Assay-dependent
-
Product Specifications

Species Reactivity

Bovine, Dog, Guinea pig, Hamster, Human, Mouse, Non-human primate, Pig, Rabbit, Rat

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

1F12-A6

Immunogen

Human HO-1 synthetic peptide, amino acids 1-30
View immunogen

Conjugate

PerCP PerCP PerCP

Excitation/Emission Max

482/675 nm View spectra spectra

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

95.64mM phosphate/2.48mM MES, pH 7.2, with 0.5M EDTA

Contains

no preservative

Storage conditions

4°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2931608

Product Specific Information

1 µg/mL was sufficient for detection of HO-1 in 10 µg of mixed human cell line lysate by colorimetric immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary.|Detects 32kDa. Does not cross-react with HO-2.

Target Information

Heme oxygenase (HO) is a microsomal enzyme that catalyzes the oxidation of heme to the antioxidant molecules, biliverdin and carbon monoxide. HO consists of two homologous isozymes, an inducible HO-1 and a constitutively expressed HO-2. HO-1 is induced by a wide variety of stimuli including conditions of oxidative stress, inflammatory agents, transforming growth factor beta and heat shock. The increase in expression of HO-1 is thought to be a cellular defense mechanism against oxidative stress since elevated HO could eventually generate more bilirubin, an anti-oxidant.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: heat shock protein 32; heat shock protein, 32-kD; heme orygenase 1; heme oxygenase (decycling) 1; heme oxygenase (decyclizing) 1; Heme oxygenase 1; hemoxygenase; HO-1; HSP32; P32 protein

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Gene Aliases: bK286B10; BOS_5643; D8Wsu38e; Hemox; Heox; HEOXG; Hmox; HMOX1; HMOX1D; HO; HO-1; HO1; HSP32

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UniProt ID: (Human) P09601, (Pig) P32394, (Bovine) Q5E9F2, (Rat) P06762, (Mouse) P14901

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Entrez Gene ID: (Human) 3162, (Pig) 445512, (Dog) 442987, (Bovine) 513221, (Rabbit) 100008919, (Guinea pig) 100715748, (Rat) 24451, (Chinese hamster) 100756872, (Mouse) 15368

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Function(s)
heme oxygenase (decyclizing) activity phospholipase D activity signal transducer activity protein binding enzyme binding heme binding protein homodimerization activity metal ion binding oxidoreductase activity
Process(es)
angiogenesis endothelial cell proliferation wound healing involved in inflammatory response negative regulation of leukocyte migration heme oxidation cellular iron ion homeostasis response to oxidative stress small GTPase mediated signal transduction excretion regulation of blood pressure cell death intrinsic apoptotic signaling pathway in response to DNA damage negative regulation of muscle cell apoptotic process smooth muscle hyperplasia cellular response to nutrient negative regulation of mast cell cytokine production erythrocyte homeostasis low-density lipoprotein particle clearance regulation of transcription from RNA polymerase II promoter in response to iron cellular response to heat response to nicotine intracellular signal transduction heme catabolic process response to hydrogen peroxide positive regulation of I-kappaB kinase/NF-kappaB signaling negative regulation of mast cell degranulation negative regulation of DNA binding negative regulation of sequence-specific DNA binding transcription factor activity negative regulation of neuron apoptotic process regulation of transcription from RNA polymerase II promoter in response to oxidative stress response to estrogen positive regulation of chemokine biosynthetic process regulation of angiogenesis positive regulation of angiogenesis positive regulation of vasodilation positive regulation of smooth muscle cell proliferation negative regulation of smooth muscle cell proliferation regulation of sequence-specific DNA binding transcription factor activity protein homooligomerization iron ion homeostasis cellular response to arsenic-containing substance cellular response to cadmium ion cellular response to hypoxia negative regulation of extrinsic apoptotic signaling pathway via death domain receptors response to hypoxia phospholipid metabolic process negative regulation of cell proliferation heme metabolic process apoptotic process oxidation-reduction process negative regulation of apoptotic process
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