Invitrogen

HPV Type 16 E7 Monoclonal Antibody (8C9)

HPV Type 16 E7 Antibody in Western Blot (WB)

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HPV Type 16 E7 Antibody (28-0006) in WB

Journal of virology 2017 - FIG 6 Confirmation of HPV16 E6, E7, and L2 protein expression by the hCRT-HPV16E6E7L2 vaccine. 293TT cells were transiently transfected by using the clinical-grade DNA vaccine hCRT-HPV16E6E7L2, harvested, and probed with monoclonal antibodies specific for HPV16 E6, E7, or L2 by Western blot analysis. Transfected cells (+), but not control cells (-), were reactive to antibodies against all three HPV16 anti... View More

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Product Details

28-0006

Applications

Tested Dilution

Publications

Western Blot (WB)

1:100

View 19 publications 19 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:2

Immunocytochemistry (ICC/IF)

View 6 publications 6 publications

Immunoprecipitation (IP)

1:10

View 2 publications 2 publications

Miscellaneous PubMed (MISC)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Virus

Published species

Virus

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

8C9

Immunogen

Bacterially derived fusion protein containing the human papillomavirus tp 16 early protein E7 open reading frame.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

50 µg/mL

Purification

HPLC

Storage buffer

PBS with 1% BSA

Contains

0.1% sodium azide

Storage conditions

4°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2533057

Product Specific Information

In Western blot against the HPV16-E7 bacterially derived fusion protein, the antibody detects a 52 kD band which is composed of the HPV16-E7 protein (15 kDa) and the trpE protein (37 kDa). Positive reactivity for the 15 kDa HPV16-E7 expressed protein was shown with CaSki (HPV16 DNA-containing cell line) cells by Western blot. The antibody has also been shown to immunoprecipitate and immunocytochemically stain the HPV16-E7 expressed protein from the CaSki cell line. Negative reactivity by Western blot was shown with a series of other HPV fusion proteins including: HPV16-E2, HPV16-E4, HPV16-E6, HPV18-E7, HPV6-E7, HPV6-E4, and HPV6-L1. The antibody failed to react with HeLa (HPV18 DNA-containing cell line) cells and other HPV DNA negative cervical carcinoma cell lines, HT-3 and C-33A, by Western blot, by immunoprecipitation or by immunocytochemical staining analyses.

Reconstitute the lyophilized material with 1 mL of deionized or distilled water for a final concentration of 50 µg/mL.

Target Information

The HPV E7 proteins are small (HPV16 E7 comprising 98 amino acids, 15kDa), zinc binding phosphoproteins which are localised in the nucleus. They are structurally and functionally similar to the E1A protein of subgenus C adenoviruses. The first 16 amino-terminal amino acids of HPV16 E7 contain a region homologous to a segment of the conserved region 1 (CR1) of the E1A protein of subgenus C adenoviruses. The next domain, up to amino acid 37, is homologous to the entire region 2 (CR2) of E1A. Genetic studies have established that these domains are required for cell transformation in vitro, suggesting similarities in the mechanism of transformation by these viruses. The CR2 homology region contains the LXCXE motif (residues 22-26) involved in binding to the tumour suppressor protein pRb.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

HPV16 E6/E7 stabilize PGK1 protein by reducing its poly-ubiquitination in cervical cancer.

Cell biology international

Liu S,Song L,Yao H,Zhang L

28-0006 was used in Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot to show that PGK1 expression might serve as a prognostic biomarker in cervical cancer.

Tue Mar 01 00:00:00 UTC 2022

Preclinical Development of Oncolytic Immunovirotherapy for Treatment of HPV^POS Cancers.

Molecular therapy oncolytics

Suksanpaisan L,Xu R,Tesfay MZ,Bomidi C,Hamm S,Vandergaast R,Jenks N,Steele MB,Ota-Setlik A,Akhtar H,Luckay A,Nowak R,Peng KW,Eldridge JH,Clarke DK,Russell SJ,Diaz RM

28-0006 was used in Western Blotting to study the use of vesicular stomatitis virus as a platform for the oncolytic immunovirotherapy of tumours expressing HPV target antigens.

Fri Sep 28 00:00:00 UTC 2018

GRWD1 negatively regulates p53 via the RPL11-MDM2 pathway and promotes tumorigenesis.

EMBO reports

Kayama K,Watanabe S,Takafuji T,Tsuji T,Hironaka K,Matsumoto M,Nakayama KI,Enari M,Kohno T,Shiraishi K,Kiyono T,Yoshida K,Sugimoto N,Fujita M

28-0006 was used in Western Blotting to show that glutamate-rich WD40 repeat containing 1 physically and functionally interacts with ribosomal protein L11.

Sun Jan 01 00:00:00 UTC 2017

Development of a replication-deficient adenoviral vector-based vaccine candidate for the interception of HPV16- and HPV18-induced infections and disease.

International journal of cancer

Khan S,Oosterhuis K,Wunderlich K,Bunnik EM,Bhaggoe M,Boedhoe S,Karia S,Steenbergen RDM,Bosch L,Serroyen J,Janssen S,Schuitemaker H,Vellinga J,Scheper G,Zahn R,Custers J

28-0006 was used in Western Blot to study the development and pre-clinical testing of a vaccine candidate consisting of replication-deficient adenovirus type 26 and 35 based vectors for the interception of HPV16- and HPV18-related disease.

Sat Jul 15 00:00:00 UTC 2017

Development of a replication-deficient adenoviral vector-based vaccine candidate for the interception of HPV16- and HPV18-induced infections and disease.

International journal of cancer

Khan S,Oosterhuis K,Wunderlich K,Bunnik EM,Bhaggoe M,Boedhoe S,Karia S,Steenbergen RDM,Bosch L,Serroyen J,Janssen S,Schuitemaker H,Vellinga J,Scheper G,Zahn R,Custers J

Sat Jul 15 00:00:00 UTC 2017

Spontaneous and Vaccine-Induced Clearance of Mus Musculus Papillomavirus 1 Infection.

Journal of virology

Jiang RT,Wang JW,Peng S,Huang TC,Wang C,Cannella F,Chang YN,Viscidi RP,Best SRA,Hung CF,Roden RBS

Published figure using HPV Type 16 E7 monoclonal antibody (Product # 28-0006) in Western Blot

Tue Aug 01 00:00:00 UTC 2017

Loss of Keratin 17 induces tissue-specific cytokine polarization and cellular differentiation in HPV16-driven cervical tumorigenesis in vivo.

Oncogene

Hobbs RP,Batazzi AS,Han MC,Coulombe PA

28-0006 was used in western blot to analyze the induction of tissue-specific cytokine polarization and cellular differentiation in HPV16-driven cervical tumorigenesis in vivo due to loss of keratin 17

Thu Oct 27 00:00:00 UTC 2016

The Human Papillomavirus 16 E7 Oncoprotein Attenuates AKT Signaling To Promote Internal Ribosome Entry Site-Dependent Translation and Expression of c-MYC.

Journal of virology

Strickland SW,Vande Pol S

28-0006 was used in Western Blotting to study how human papillomavirus oncoproteins E6 and E7 manipulate cellular signalling cascades to promote the viral life cycle and cancer development.

Wed Jun 15 00:00:00 UTC 2016

Keratin-dependent regulation of Aire and gene expression in skin tumor keratinocytes.

Nature genetics

Hobbs RP,DePianto DJ,Jacob JT,Han MC,Chung BM,Batazzi AS,Poll BG,Guo Y,Han J,Ong S,Zheng W,Taube JM,Čiháková D,Wan F,Coulombe PA

28-0006 was used in immunoprecipitation and western blot to report that autoimmune regulator is induced in human and mouse tumor keratinocytes in a K17-dependent manner and results in Gli2-induced skin tumorigenesis in mice

Sat Aug 01 00:00:00 UTC 2015

A functional interaction of E7 with B-Myb-MuvB complex promotes acute cooperative transcriptional activation of both S- and M-phase genes. (129 c).

Oncogene

Pang CL,Toh SY,He P,Teissier S,Ben Khalifa Y,Xue Y,Thierry F

28-0006 was used in western blot to study the interaction of human papillomaviruse E7 protein with B-Myb-MuvB complex and its effect on gene expression

Thu Jul 31 00:00:00 UTC 2014

Human papillomavirus 16 oncoprotein E7 stimulates UBF1-mediated rDNA gene transcription, inhibiting a p53-independent activity of p14ARF.

PloS one

Dichamp I,Séité P,Agius G,Barbarin A,Beby-Defaux A

28-0006 was used in immunoprecipitation to study the interaction between p14ARF and the human papillomavirus oncoprotein E7

Fri Jun 12 00:00:00 UTC 2015

Successful therapeutic vaccination with integrase defective lentiviral vector expressing nononcogenic human papillomavirus E7 protein.

International journal of cancer

Grasso F,Negri DR,Mochi S,Rossi A,Cesolini A,Giovannelli A,Chiantore MV,Leone P,Giorgi C,Cara A

Tue Jan 15 00:00:00 UTC 2013

Successful therapeutic vaccination with integrase defective lentiviral vector expressing nononcogenic human papillomavirus E7 protein.

International journal of cancer

Grasso F,Negri DR,Mochi S,Rossi A,Cesolini A,Giovannelli A,Chiantore MV,Leone P,Giorgi C,Cara A

Tue Jan 15 00:00:00 UTC 2013

Human papillomavirus E7 induces rereplication in response to DNA damage.

Journal of virology

Fan X,Liu Y,Heilman SA,Chen JJ

Published figure using HPV Type 16 E7 monoclonal antibody (Product # 28-0006) in Western Blot

Tue Jan 01 00:00:00 UTC 2013

Reconstruction of human papillomavirus type 16-mediated early-stage neoplasia implicates E6/E7 deregulation and the loss of contact inhibition in neoplastic progression.

Journal of virology

Isaacson Wechsler E,Wang Q,Roberts I,Pagliarulo E,Jackson D,Untersperger C,Coleman N,Griffin H,Doorbar J

Fri Jun 01 00:00:00 UTC 2012

Reconstruction of human papillomavirus type 16-mediated early-stage neoplasia implicates E6/E7 deregulation and the loss of contact inhibition in neoplastic progression.

Journal of virology

Isaacson Wechsler E,Wang Q,Roberts I,Pagliarulo E,Jackson D,Untersperger C,Coleman N,Griffin H,Doorbar J

Fri Jun 01 00:00:00 UTC 2012

The E7 open reading frame acts in cis and in trans to mediate differentiation-dependent activities in the human papillomavirus type 16 life cycle.

Journal of virology

Bodily JM,Mehta KP,Cruz L,Meyers C,Laimins LA

Published figure using HPV Type 16 E7 monoclonal antibody (Product # 28-0006) in Western Blot

Thu Sep 01 00:00:00 UTC 2011

Localisation of human papillomavirus 16 E7 oncoprotein changes with cell confluence.

PloS one

Laurson J,Raj K

28-0006 was used in immunocytochemistry and western blot to investigate the localization of E7 from human papillomavirus type 16

Thu Dec 01 00:00:00 UTC 2011

An in vitro multistep carcinogenesis model for both HPV-positive and -negative human oral squamous cell carcinomas.

American journal of cancer research

Zushi Y,Narisawa-Saito M,Noguchi K,Yoshimatsu Y,Yugawa T,Egawa N,Fujita M,Urade M,Kiyono T

28-0006 was used in Western Blot to indicate that either HRAS mutation or activation of EGFR in cooperation with MYC overexpression play critical roles in transformation of HTKs on a background of inactivation of the pRB and p53 pathways and telomerase activation.

Thu Nov 10 00:00:00 UTC 2011

Differential sensitivity of the inner ear sensory cell populations to forced cell cycle re-entry and p53 induction.

Journal of neurochemistry

Sulg M,Kirjavainen A,Pajusola K,Bueler H,Ylikoski J,Laiho M,Pirvola U

28-0006 was used in immunocytochemistry to study the response of auditory and balance hair cells to forced cell cycle reactivation and p53 up-regulation.

Mon Mar 01 00:00:00 UTC 2010

E6AP-dependent degradation of DLG4/PSD95 by high-risk human papillomavirus type 18 E6 protein.

Journal of virology

Handa K,Yugawa T,Narisawa-Saito M,Ohno S,Fujita M,Kiyono T

28-0006 was used in Western Blotting to suggest that DLG4 may function as a tumor suppressor in the development of HPV-associated cancers.

Thu Feb 01 00:00:00 UTC 2007

Cervical keratinocytes containing stably replicating extrachromosomal HPV-16 are refractory to transformation by oncogenic H-Ras.

Virology

Berger KL,Barriga F,Lace MJ,Turek LP,Zamba GJ,Domann FE,Lee JH,Klingelhutz AJ

Published figure using HPV Type 16 E7 monoclonal antibody (Product # 28-0006) in Western Blot

Fri Jan 12 00:00:00 UTC 2007

Two distinct activities contribute to human papillomavirus 16 E6's oncogenic potential.

Cancer research

Simonson SJ,Difilippantonio MJ,Lambert PF

Thu Sep 15 00:00:00 UTC 2005

Cyclin G1 has growth inhibitory activity linked to the ARF-Mdm2-p53 and pRb tumor suppressor pathways.

Molecular cancer research : MCR

Zhao L,Samuels T,Winckler S,Korgaonkar C,Tompkins V,Horne MC,Quelle DE

Wed Jan 01 00:00:00 UTC 2003

The role of apoptosis in creating and maintaining luminal space within normal and oncogene-expressing mammary acini.

Cell

Debnath J,Mills KR,Collins NL,Reginato MJ,Muthuswamy SK,Brugge JS

Fri Oct 04 00:00:00 UTC 2002

The HPV E7 oncoprotein inhibits tumor necrosis factor alpha-mediated apoptosis in normal human fibroblasts.

Oncogene

Thompson DA,Zacny V,Belinsky GS,Classon M,Jones DL,Schlegel R,Münger K

Thu Jun 21 00:00:00 UTC 2001

The HPV E7 oncoprotein inhibits tumor necrosis factor alpha-mediated apoptosis in normal human fibroblasts.

Oncogene

Thompson DA,Zacny V,Belinsky GS,Classon M,Jones DL,Schlegel R,Münger K

Thu Jun 21 00:00:00 UTC 2001

Degradation of the retinoblastoma tumor suppressor by the human papillomavirus type 16 E7 oncoprotein is important for functional inactivation and is separable from proteasomal degradation of E7.

Journal of virology

Gonzalez SL,Stremlau M,He X,Basile JR,Münger K

28-0006 was used in Western Blot to demonstrate that pRB degradation is a direct activity of E7 and does not reflect a property of cell lines acquired during the selection process for E7 expression.

Wed Aug 01 00:00:00 UTC 2001

Preservation of the myofibroblastic phenotype of human papilloma virus 16 E6/E7 immortalized human bone marrow cells using the lineage limited alpha-smooth muscle actin promoter.

Cell growth & differentiation : the molecular biology journal of the American Association for Cancer Research

Loeuillet C,Douay L,Hervé P,Chalmers DE

Tue May 01 00:00:00 UTC 2001

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HPV Type 16 E7 Monoclonal Antibody (8C9)

HPV Type 16 E7 Antibody (28-0006) in WB

Product Details

28-0006

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Miscellaneous PubMed (MISC)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Performance Guarantee

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