Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
FIGURE: 1 / 1
HR23A Antibody (600-101-291) in WB
Western blot showing Rocklands Affinity Purified anti-HR23A antibody detects endogenous human HR23A. Reactivity is shown against HeLa nuclear extract (lane 1) and HeLa (lane 2), A431 (lane 3), Jurkat (lane 4) and 293 whole cell lysates (lane 5). Comparison to a molecular weight marker (at left) indicates a band of ~60 kDa corresponding to HR23A. The blot was incubated with a 1:500 dilution of the antibody at room temperature followed by detection using HRP conjugated Rb-a-Goat IgG and chemiluminescence reagent with a 30-min exposure time. ... View More
Product Details
600-101-291
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Goat
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
This affinity purified antibody was prepared from whole goat serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near aa 115-140 of human HR23A protein.
Conjugate
Form
Liquid
Concentration
1.2 mg/mL
Purification
Affinity chromatography
Storage buffer
0.02M potassium phosphate, pH 7.2, with 0.15M NaCl
Contains
0.01% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Reactivity occurs against human HR23A protein. Sequence homology as assessed by BLAST indicated 100% homology for this protein from human and rabbit. Cross reactivity with HR23A protein from dog, mouse and rat may also occur as sequence homology varies by one or two amino acid residues in this sequence by BLAST analysis. Reactivity with HR23A protein from other sources is not known.
Target Information
The product of this gene is a small iron-sulfur protein that transfers electrons from NADPH through ferredoxin reductase to a terminal cytochrome P450. This particular oxidation/reduction system is found in steroidogenic tissues, and is involved with the synthesis of bile acid and vitamin D. In addition to the expressed gene at this chromosomal locus (11q22), there are pseudogenes located on chromosomes 20 and 21. This gene product has been identified in a number of different tissues but all forms have been shown to be identical and are not tissue specific.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: HR23A; MGC111083; rad23; RAD23, yeast homolog, A; UV excision repair protein RAD23 homolog A
Gene Aliases: HHR23A; HR23A; RAD23A
UniProt ID: (Human) P54725
Entrez Gene ID: (Human) 5886
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support