Proteintech

HRAS Polyclonal Antibody

HRAS Antibody in Immunocytochemistry (ICC/IF)

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HRAS Antibody (18295-1-AP) in ICC/IF

Immunofluorescent analysis of HepG2 cells using 18295-1-AP (HRAS-Specific antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

HRAS Antibody in Immunohistochemistry (Paraffin) (IHC (P))

HRAS Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

18295-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 38 publications 38 publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

1:10-1:100

View 3 publications 3 publications

Flow Cytometry (Flow)

1 µg/1x10^6 cells

Immunoprecipitation (IP)

0.5-4.0 µg

View 1 publication 1 publication

Not applicable (N/A)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Peptide

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.31 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

The antibody is specific to HRAS and does not recognize NRAS and KRAS.

Target Information

Monomeric G protein Ras functions as a molecular switch linking receptor and nonreceptor tyrosine kinase activation to a number of downstream signaling pathways leading to distinct cytoplasmic or nuclear events. Each mammalian cell contains three Ras proto-oncogene coding for closely related Ras proteins: H-, K-, N-Ras. Oncogenic mutations in Ras genes are present in ~30% of all human cancers. Constitutive activation of Ras due to mutations or overexpression stimulates proliferation and inhibition of apoptosis. K-Ras mutations are common in pancreatic, colorectal and nonsmall-cell lung carcinomas; H-Ras mutations are common in bladder, kidney and thyroid carcinomas; N-Ras mutations are found in melanoma, hematological malignancies and hepatocellular carcinaoma.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Pan-KRAS inhibitor disables oncogenic signalling and tumour growth.

Nature

Kim D,Herdeis L,Rudolph D,Zhao Y,Böttcher J,Vides A,Ayala-Santos CI,Pourfarjam Y,Cuevas-Navarro A,Xue JY,Mantoulidis A,Bröker J,Wunberg T,Schaaf O,Popow J,Wolkerstorfer B,Kropatsch KG,Qu R,de Stanchina E,Sang B,Li C,McConnell DB,Kraut N,Lito P

Sat Jul 01 00:00:00 UTC 2023

Genome-wide CRISPR/Cas9 screens reveal shared and cell-specific mechanisms of resistance to SHP2 inhibition.

The Journal of experimental medicine

Wei W,Geer MJ,Guo X,Dolgalev I,Sanjana NE,Neel BG

Mon May 01 00:00:00 UTC 2023

1-Indanone retards cyst development in ADPKD mouse model by stabilizing tubulin and down-regulating anterograde transport of cilia.

Acta pharmacologica Sinica

Li XW,Ran JH,Zhou H,He JZ,Qiu ZW,Wang SY,Wu MN,Zhu S,An YP,Ma A,Li M,Quan YZ,Li NN,Ren CQ,Yang BX

Wed Feb 01 00:00:00 UTC 2023

Deletion of Small GTPase H-Ras Rescues Memory Deficits and Reduces Amyloid Plaque-Associated Dendritic Spine Loss in Transgenic Alzheimer's Mice.

Molecular neurobiology

Qu W,Jeong A,Zhong R,Thieschafer JS,Gram A,Li L

Wed Feb 01 00:00:00 UTC 2023

Cutaneous manifestations in Costello syndrome: HRAS p.Gly12Ser affects RIN1-mediated integrin trafficking in immortalized epidermal keratinocytes.

Human molecular genetics

Nauth T,Bazgir F,Voß H,Brandenstein LI,Mosaddeghzadeh N,Rickassel V,Deden S,Gorzelanny C,Schlüter H,Ahmadian MR,Rosenberger G

Fri Jan 06 00:00:00 UTC 2023

Epitranscriptic regulation of HRAS by N⁶-methyladenosine drives tumor progression.

Proceedings of the National Academy of Sciences of the United States of America

Pan Y,Gu Y,Liu T,Zhang Q,Yang F,Duan L,Cheng S,Zhu X,Xi Y,Chang X,Ye Q,Gao S

Tue Apr 04 00:00:00 UTC 2023

Epitranscriptic regulation of HRAS by N⁶-methyladenosine drives tumor progression.

Proceedings of the National Academy of Sciences of the United States of America

Pan Y,Gu Y,Liu T,Zhang Q,Yang F,Duan L,Cheng S,Zhu X,Xi Y,Chang X,Ye Q,Gao S

Tue Apr 04 00:00:00 UTC 2023

RAB27B controls palmitoylation-dependent NRAS trafficking and signaling in myeloid leukemia.

The Journal of clinical investigation

Ren JG,Xing B,Lv K,O'Keefe RA,Wu M,Wang R,Bauer KM,Ghazaryan A,Burslem GM,Zhang J,O'Connell RM,Pillai V,Hexner EO,Philips MR,Tong W

Thu Jun 15 00:00:00 UTC 2023

Targeting farnesylation as a novel therapeutic approach in HRAS-mutant rhabdomyosarcoma.

Oncogene

Odeniyide P,Yohe ME,Pollard K,Vaseva AV,Calizo A,Zhang L,Rodriguez FJ,Gross JM,Allen AN,Wan X,Somwar R,Schreck KC,Kessler L,Wang J,Pratilas CA

Sun May 01 00:00:00 UTC 2022

Targeting farnesylation as a novel therapeutic approach in HRAS-mutant rhabdomyosarcoma.

Oncogene

Odeniyide P,Yohe ME,Pollard K,Vaseva AV,Calizo A,Zhang L,Rodriguez FJ,Gross JM,Allen AN,Wan X,Somwar R,Schreck KC,Kessler L,Wang J,Pratilas CA

Sun May 01 00:00:00 UTC 2022

Argonaute 2 modulates EGFR-RAS signaling to promote mutant HRAS and NRAS-driven malignancies.

PNAS nexus

Siebenaler RF,Chugh S,Waninger JJ,Dommeti VL,Kenum C,Mody M,Gautam A,Patel N,Chu A,Bawa P,Hon J,Smith RD,Carlson H,Cao X,Tesmer JJG,Shankar S,Chinnaiyan AM

Fri Jul 01 00:00:00 UTC 2022

Validation of a small molecule inhibitor of PDE6D-RAS interaction with favorable anti-leukemic effects.

Blood cancer journal

Canovas Nunes S,De Vita S,Anighoro A,Autelitano F,Beaumont E,Klingbeil P,McGuinness M,Duvert B,Harris C,Yang L,Pokharel SP,Chen CW,Ermann M,Williams DA,Xu H

Thu Apr 14 00:00:00 UTC 2022

Depleting receptor tyrosine kinases EGFR and HER2 overcomes resistance to EGFR inhibitors in colorectal cancer.

Journal of experimental & clinical cancer research : CR

Yang L,Bhattacharya A,Li Y,Sexton S,Ling X,Li F,Zhang Y

Thu Jun 02 00:00:00 UTC 2022

Ionic Liquid-Based Extraction System for In-Depth Analysis of Membrane Protein Complexes.

Analytical chemistry

Chu H,Zhao Q,Liu J,Yang K,Wang Y,Liu J,Zhang K,Zhao B,He H,Zheng Y,Zhong S,Liang Z,Zhang L,Zhang Y

Tue Jan 18 00:00:00 UTC 2022

KRAS^G12C-independent feedback activation of wild-type RAS constrains KRAS^G12C inhibitor efficacy.

Cell reports

Ryan MB,Coker O,Sorokin A,Fella K,Barnes H,Wong E,Kanikarla P,Gao F,Zhang Y,Zhou L,Kopetz S,Corcoran RB

Tue Jun 21 00:00:00 UTC 2022

Xiao-Luo-Wan treats propylthiouracil-induced goiter with hypothyroidism in rats through the PI3K-AKT/RAS pathways based on UPLC/MS and network pharmacology.

Journal of ethnopharmacology

Dong S,Liu Q,Jiang M,Ma Q,Huang Q,Liu T,Li Y,Ni L,Shi Y

Tue May 10 00:00:00 UTC 2022

Inhibition of mu-opioid receptor suppresses proliferation of hepatocellular carcinoma cells via CD147-p53-MAPK cascade signaling pathway.

American journal of translational research

Zhang JJ,Song CG,Dai JM,Zhang XQ,Lin P,Li L,Yang XM,Chen ZN

Tue Jun 22 00:00:00 UTC 2021

Aging-associated lncRNAs are evolutionarily conserved and participate in NFκB signaling.

Nature aging

Cai D,Han JJ

Sat May 01 00:00:00 UTC 2021

Validation of Isoform- and Mutation-Specific RAS Antibodies.

Methods in molecular biology (Clifton, N.J.)

Waters AM,Der CJ

Thu Jun 17 00:00:00 UTC 2021

KARATE: PKA-induced KRAS4B-RHOA-mTORC2 supercomplex phosphorylates AKT in insulin signaling and glucose homeostasis.

Molecular cell

Senoo H,Murata D,Wai M,Arai K,Iwata W,Sesaki H,Iijima M

Thu Nov 18 00:00:00 UTC 2021

Combinations with Allosteric SHP2 Inhibitor TNO155 to Block Receptor Tyrosine Kinase Signaling.

Clinical cancer research : an official journal of the American Association for Cancer Research

Liu C,Lu H,Wang H,Loo A,Zhang X,Yang G,Kowal C,Delach S,Wang Y,Goldoni S,Hastings WD,Wong K,Gao H,Meyer MJ,Moody SE,LaMarche MJ,Engelman JA,Williams JA,Hammerman PS,Abrams TJ,Mohseni M,Caponigro G,Hao HX

Fri Jan 01 00:00:00 UTC 2021

Erianin, the main active ingredient of Dendrobium chrysotoxum Lindl, inhibits precancerous lesions of gastric cancer (PLGC) through suppression of the HRAS-PI3K-AKT signaling pathway as revealed by network pharmacology and in vitro experimental verification.

Journal of ethnopharmacology

Wang Y,Chu F,Lin J,Li Y,Johnson N,Zhang J,Gai C,Su Z,Cheng H,Wang L,Ding X

Thu Oct 28 00:00:00 UTC 2021

Protein farnesylation is upregulated in Alzheimer's human brains and neuron-specific suppression of farnesyltransferase mitigates pathogenic processes in Alzheimer's model mice.

Acta neuropathologica communications

Jeong A,Cheng S,Zhong R,Bennett DA,Bergö MO,Li L

Tue Jul 27 00:00:00 UTC 2021

Targeting Endogenous K-RAS for Degradation through the Affinity-Directed Protein Missile System.

Cell chemical biology

Röth S,Macartney TJ,Konopacka A,Chan KH,Zhou H,Queisser MA,Sapkota GP

Thu Sep 17 00:00:00 UTC 2020

Immunohistochemical assessment of growth factor signaling molecules: MAPK, Akt, and STAT3 pathways in oral epithelial precursor lesions and squamous cell carcinoma.

Odontology

Tashiro K,Oikawa M,Miki Y,Takahashi T,Kumamoto H

Wed Jan 01 00:00:00 UTC 2020

H-Ras activation and fibroblast-induced TGF-β signaling promote laminin-332 accumulation and invasion in cutaneous squamous cell carcinoma.

Matrix biology : journal of the International Society for Matrix Biology

Siljamäki E,Rappu P,Riihilä P,Nissinen L,Kähäri VM,Heino J

Fri May 01 00:00:00 UTC 2020

Analysis of RAS protein interactions in living cells reveals a mechanism for pan-RAS depletion by membrane-targeted RAS binders.

Proceedings of the National Academy of Sciences of the United States of America

Li YC,Lytle NK,Gammon ST,Wang L,Hayes TK,Sutton MN,Bast RC,Der CJ,Piwnica-Worms D,McCormick F,Wahl GM

Tue Jun 02 00:00:00 UTC 2020

Vertical Pathway Inhibition Overcomes Adaptive Feedback Resistance to KRAS^G12C Inhibition.

Clinical cancer research : an official journal of the American Association for Cancer Research

Ryan MB,Fece de la Cruz F,Phat S,Myers DT,Wong E,Shahzade HA,Hong CB,Corcoran RB

Wed Apr 01 00:00:00 UTC 2020

Cell-type specific tumorigenesis with Ras oncogenes in human lung epithelial cells.

Biochemical and biophysical research communications

Kumazaki M,Shimomura I,Kiyono T,Ochiya T,Yamamoto Y

Thu Apr 30 00:00:00 UTC 2020

Effects of mevalonate kinase interference on cell differentiation, apoptosis, prenylation and geranylgeranylation of human keratinocytes are attenuated by farnesyl pyrophosphate or geranylgeranyl pyrophosphate.

Experimental and therapeutic medicine

Li M,Min W,Wang J,Wang L,Li Y,Zhou N,Yang Z,Qian Q

Wed Apr 01 00:00:00 UTC 2020

A potent KRAS macromolecule degrader specifically targeting tumours with mutant KRAS.

Nature communications

Bery N,Miller A,Rabbitts T

Fri Jun 26 00:00:00 UTC 2020

KRAS regulation by small non-coding RNAs and SNARE proteins.

Nature communications

Che Y,Siprashvili Z,Kovalski JR,Jiang T,Wozniak G,Elcavage L,Khavari PA

Mon Nov 11 00:00:00 UTC 2019

A systems mechanism for KRAS mutant allele-specific responses to targeted therapy.

Science signaling

McFall T,Diedrich JK,Mengistu M,Littlechild SL,Paskvan KV,Sisk-Hackworth L,Moresco JJ,Shaw AS,Stites EC

Tue Sep 24 00:00:00 UTC 2019

The ERBB network facilitates KRAS-driven lung tumorigenesis.

Science translational medicine

Kruspig B,Monteverde T,Neidler S,Hock A,Kerr E,Nixon C,Clark W,Hedley A,Laing S,Coffelt SB,Le Quesne J,Dick C,Vousden KH,Martins CP,Murphy DJ

Wed Jun 20 00:00:00 UTC 2018

The ERBB network facilitates KRAS-driven lung tumorigenesis.

Science translational medicine

Kruspig B,Monteverde T,Neidler S,Hock A,Kerr E,Nixon C,Clark W,Hedley A,Laing S,Coffelt SB,Le Quesne J,Dick C,Vousden KH,Martins CP,Murphy DJ

Wed Jun 20 00:00:00 UTC 2018

KRAS Oncoprotein Expression Is Regulated by a Self-Governing eIF5A-PEAK1 Feed-Forward Regulatory Loop.

Cancer research

Fujimura K,Wang H,Watson F,Klemke RL

Thu Mar 15 00:00:00 UTC 2018

Nogo-B receptor promotes epithelial-mesenchymal transition in non-small cell lung cancer cells through the Ras/ERK/Snail1 pathway.

Cancer letters

Wu D,Zhao B,Qi X,Peng F,Fu H,Chi X,Miao QR,Shao S

Sun Apr 01 00:00:00 UTC 2018

Nogo-B receptor promotes epithelial-mesenchymal transition in non-small cell lung cancer cells through the Ras/ERK/Snail1 pathway.

Cancer letters

Wu D,Zhao B,Qi X,Peng F,Fu H,Chi X,Miao QR,Shao S

Sun Apr 01 00:00:00 UTC 2018

Proteomic Analysis of Lysosomal Membrane Proteins in Bovine Mammary Epithelial Cells Illuminates Potential Novel Lysosome Functions in Lactation.

Journal of agricultural and food chemistry

Luo C,Zhao S,Dai W,Zheng N,Wang J

Wed Dec 12 00:00:00 UTC 2018

Targeting KRAS-dependent tumors with AZD4785, a high-affinity therapeutic antisense oligonucleotide inhibitor of KRAS.

Science translational medicine

Ross SJ,Revenko AS,Hanson LL,Ellston R,Staniszewska A,Whalley N,Pandey SK,Revill M,Rooney C,Buckett LK,Klein SK,Hudson K,Monia BP,Zinda M,Blakey DC,Lyne PD,Macleod AR

Wed Jun 14 00:00:00 UTC 2017

Targeting KRAS-dependent tumors with AZD4785, a high-affinity therapeutic antisense oligonucleotide inhibitor of KRAS.

Science translational medicine

Ross SJ,Revenko AS,Hanson LL,Ellston R,Staniszewska A,Whalley N,Pandey SK,Revill M,Rooney C,Buckett LK,Klein SK,Hudson K,Monia BP,Zinda M,Blakey DC,Lyne PD,Macleod AR

Wed Jun 14 00:00:00 UTC 2017

Evaluation of the selectivity and sensitivity of isoform- and mutation-specific RAS antibodies.

Science signaling

Waters AM,Ozkan-Dagliyan I,Vaseva AV,Fer N,Strathern LA,Hobbs GA,Tessier-Cloutier B,Gillette WK,Bagni R,Whiteley GR,Hartley JL,McCormick F,Cox AD,Houghton PJ,Huntsman DG,Philips MR,Der CJ

Tue Sep 26 00:00:00 UTC 2017

Transformation-induced changes in the DNA-nuclear matrix interface, revealed by high-throughput analysis of DNA halos.

Scientific reports

Wilson RHC,Coverley D

Tue Jul 25 00:00:00 UTC 2017

Transformation-induced changes in the DNA-nuclear matrix interface, revealed by high-throughput analysis of DNA halos.

Scientific reports

Wilson RHC,Coverley D

Tue Jul 25 00:00:00 UTC 2017

MacroH2A1 downregulation enhances the stem-like properties of bladder cancer cells by transactivation of Lin28B.

Oncogene

Park SJ,Shim JW,Park HS,Eum DY,Park MT,Mi Yi J,Choi SH,Kim SD,Son TG,Lu W,Kim ND,Yang K,Heo K

Thu Mar 10 00:00:00 UTC 2016

MacroH2A1 downregulation enhances the stem-like properties of bladder cancer cells by transactivation of Lin28B.

Oncogene

Park SJ,Shim JW,Park HS,Eum DY,Park MT,Mi Yi J,Choi SH,Kim SD,Son TG,Lu W,Kim ND,Yang K,Heo K

Thu Mar 10 00:00:00 UTC 2016

Aspafilioside B induces G2/M cell cycle arrest and apoptosis by up-regulating H-Ras and N-Ras via ERK and p38 MAPK signaling pathways in human hepatoma HepG2 cells.

Molecular carcinogenesis

Liu W,Ning R,Chen RN,Huang XF,Dai QS,Hu JH,Wang YW,Wu LL,Xiong J,Hu G,Guo QL,Yang J,Wang H

Sun May 01 00:00:00 UTC 2016

Aspafilioside B induces G2/M cell cycle arrest and apoptosis by up-regulating H-Ras and N-Ras via ERK and p38 MAPK signaling pathways in human hepatoma HepG2 cells.

Molecular carcinogenesis

Liu W,Ning R,Chen RN,Huang XF,Dai QS,Hu JH,Wang YW,Wu LL,Xiong J,Hu G,Guo QL,Yang J,Wang H

Sun May 01 00:00:00 UTC 2016

Paper-based microreactor array for rapid screening of cell signaling cascades.

Lab on a chip

Huang CH,Lei KF,Tsang NM

Sun Aug 07 00:00:00 UTC 2016

Paper-based microreactor array for rapid screening of cell signaling cascades.

Lab on a chip

Huang CH,Lei KF,Tsang NM

Sun Aug 07 00:00:00 UTC 2016

Eicosapentaenoic acid activates RAS/ERK/C/EBPβ pathway through H-Ras intron 1 CpG island demethylation in U937 leukemia cells.

PloS one

Ceccarelli V,Nocentini G,Billi M,Racanicchi S,Riccardi C,Roberti R,Grignani F,Binaglia L,Vecchini A

Thu Sep 18 00:00:00 UTC 2014

Eicosapentaenoic acid activates RAS/ERK/C/EBPβ pathway through H-Ras intron 1 CpG island demethylation in U937 leukemia cells.

PloS one

Ceccarelli V,Nocentini G,Billi M,Racanicchi S,Riccardi C,Roberti R,Grignani F,Binaglia L,Vecchini A

Thu Sep 18 00:00:00 UTC 2014

The Wnt-β-catenin pathway represses let-7 microRNA expression through transactivation of Lin28 to augment breast cancer stem cell expansion.

Journal of cell science

Cai WY,Wei TZ,Luo QC,Wu QW,Liu QF,Yang M,Ye GD,Wu JF,Chen YY,Sun GB,Liu YJ,Zhao WX,Zhang ZM,Li BA

Mon Jul 01 00:00:00 UTC 2013

The Wnt-β-catenin pathway represses let-7 microRNA expression through transactivation of Lin28 to augment breast cancer stem cell expansion.

Journal of cell science

Cai WY,Wei TZ,Luo QC,Wu QW,Liu QF,Yang M,Ye GD,Wu JF,Chen YY,Sun GB,Liu YJ,Zhao WX,Zhang ZM,Li BA

Mon Jul 01 00:00:00 UTC 2013

Bioinformatics

Protein Aliases: c-H-ras; c-Ha-ras p21 protein; c-Ha-ras transgene; c-has/bas p21 protein; c-ras-Ki-2 activated oncogene; GTP- and GDP-binding peptide B; GTPase HRas; GTPase Hras1; H-ras 1 protein; H-Ras-1; Ha-Ras; Ha-Ras1 proto-oncoprotein; Harvey ras1 protein; Harvey rat sarcoma viral (v-Ha-ras) oncogene homolog; Harvey rat sarcoma viral oncogene homolog; Harvey rat sarcoma viral oncoprotein; Harvey rat sarcoma virus oncogene 1; p19 H-RasIDX protein; p21ras; Ras family small GTP binding protein H-Ras; transformation gene: oncogene HAMSV; Transforming protein p21; v-Ha-ras Harvey rat sarcoma viral oncogene homolog

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Gene Aliases: C-BAS/HAS; C-H-RAS; c-Ha-ras; C-HA-RAS1; c-rasHa; CTLO; H-ras; H-RASIDX; Ha-ras; HAMSV; Harvey-ras; HRAS; Hras-1; HRAS1; Kras2; p21ras; ras; RASH1

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UniProt ID: (Human) P01112, (Mouse) Q61411, (Rat) P20171

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Entrez Gene ID: (Human) 3265, (Mouse) 15461, (Rat) 293621

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Function(s)

GTPase activity protein binding GTP binding protein C-terminus binding nucleotide binding

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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HRAS Polyclonal Antibody

HRAS Antibody (18295-1-AP) in ICC/IF

Product Details

18295-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

Not applicable (N/A)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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