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Invitrogen
HRH1 Polyclonal Antibody
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FIGURE: 1 / 2
HRH1 Antibody (PA5-120691) in WB
Western blot analysis of HRH1 in extracts of various cell lines. Samples were incubated with HRH1 Polyclonal antibody (Product # PA5-120691) using a dilution of 1:3,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.
Product Details
PA5-120691
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 250-340 of human HRH1 (NP_000852.1).
Conjugate
Form
Liquid
Concentration
0.73 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2914263
Product Specific Information
Positive test controls include: U-251MG, Mouse liver, Rat liver.
Immunogen sequence: KPGKESPWEV LKRKPKDAGG GSVLKSPSQT PKEMKSPVVF SQEDDREVDK LYCFPLDIVH MQAAAEGSSR DYVAVNRSHG QLKTDEQGLN T
Target Information
Histamine is a ubiquitous messenger molecule released from mast cells, enterochromaffin-like cells, and neurons. Its various actions are mediated by histamine receptors H1, H2, H3 and H4. This gene was thought to be intronless until recently. The protein encoded by this gene is an integral membrane protein and belongs to the G protein-coupled receptor superfamily. It mediates the contraction of smooth muscles, the increase in capillary permeability due to contraction of terminal venules, the release of catecholamine from adrenal medulla, and neurotransmission in the central nervous system. Multiple alternatively spliced variants, encoding the same protein, have been identified.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Bordetella pertussis induced histamine sensitization; H1 receptor; H1-R; H1R; HH1R; Histamine H1 receptor; Histamine H1-R; histamine receptor; histamine receptor, subclass H1
Gene Aliases: Bphs; H1-R; H1R; HH1R; Hir; hisH1; Hisr; HRH1
UniProt ID: (Human) P35367, (Mouse) P70174, (Rat) P31390
Entrez Gene ID: (Human) 3269, (Mouse) 15465, (Rat) 24448
inflammatory response
G-protein coupled receptor signaling pathway
phospholipase C-activating G-protein coupled receptor signaling pathway
memory
visual learning
regulation of vasoconstriction
positive regulation of inositol trisphosphate biosynthetic process
regulation of vascular permeability
positive regulation of vasoconstriction
inositol phosphate-mediated signaling
regulation of synaptic plasticity
eosinophil chemotaxis
modulation of synaptic transmission
cellular response to histamine
signal transduction
chemical synaptic transmission
sensory perception of chemical stimulus
negative regulation of steroid biosynthetic process
positive regulation of nitric oxide biosynthetic process
rhythmic process
calcium ion import
calcium ion transmembrane transport
manganese ion transmembrane transport
G-protein coupled receptor signaling pathway coupled to cGMP nucleotide second messenger
response to gravity
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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