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Invitrogen
HSC70 Monoclonal Antibody (1B5)
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HSC70 Antibody (MA1-26078) in ICC/IF
Immunofluorescence analysis of HSC70 was performed using 70% confluent log phase PC-3 cells treated with Heat Shock (43 degree celsius for 1 hr). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with HSC70 Monoclonal Antibody (1B5) (Product # MA1-26078) at 1 µg/mL concentration in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Rat IgG (H+L) Cross-Adsorbed Second... View More
Product Details
MA1-26078
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bovine,
Dog,
Chicken,
Guinea pig,
Hamster,
Human,
Mouse,
Non-human primate,
Sheep,
Pig,
Rabbit,
Rat
Published species
Mouse
Host/Isotype
Rat
/ IgG2a
Class
Monoclonal
Type
Antibody
Clone
1B5
Immunogen
Full length native Hsc70 protein (Hamster) purified from sodium arsenite treated heat-resistant variants of Chinese hamster cells.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein G
Storage buffer
PBS, pH 7.2, with 0.1mM PMSF, 50% glycerol
Contains
no preservative
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_794523
Product Specific Information
Recommended positive controls: Bovine Hsc70 Protein and HeLa Heat Shocked Cell Lysate.
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
The HSP70 family is a set of highly conserved proteins that are induced by a variety of biological stresses, including heat stress, in every organism in which the proteins have been examined. The human HSP70 family members include: HSP70, a protein which is strongly inducible in all organisms but which is also constitutively expressed in primate cells; HSP72, a 72 kDa protein that is induced exclusively under stress conditions; HSC70, or cognate protein, is a 72 kDa, constitutively expressed, protein which is involved in the uncoating of clathrin coated vesicles; GRP78, or BiP, is a glucose regulated 78 kDa protein localized in the endoplasmic reticulum; and p75, or HSP75, a 75 kDa protein that is found within the mitochondria. HSC70 (also known as HSC71, HSC73, HSP73, p72, prp73) is expressed constitutively and is slightly heat-inducible. HSC70 binds to the exposed loop of clathrin light chains to promote uncoating and can also bind the cytoskeleton which may facilitate cytoskeletal rearrangements. HSC70 has been shown to stimulate lysosomal degradation of intracellular proteins and to retard both aggregation and folding of mitochondrial precursor proteins in vitro.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: AAG2; constitutive heat shock protein 70; epididymis luminal protein 33; epididymis secretory sperm binding protein Li 72p; FAM10A1; Heat shock 70 kDa protein 8; heat shock 70kd protein 10; heat shock 70kd protein 10 (HSC71); heat shock 70kD protein 8; heat shock 70kDa protein 8; heat shock 70kDa protein 8-like; heat shock cognate 70; heat shock cognate 71 kD protein; Heat shock cognate 71 kDa protein; heat shock cognate hsc73; heat shock cognate protein 54; Heat shock cognate protein 70; heat shock protein 8; heat shock protein A8; heat shock protein cognate 70; Heat shock protein family A member 8; HIP; Hsc70 ATPase; LAP; LAP-1; Lipopolysaccharide-associated protein 1; LPS-associated protein 1; MGC131511; MGC29929; N-myristoyltransferase inhibitor protein 71; Protein FAM10A1; SNC6
Gene Aliases: 2410008N15Rik; BOS_14437; HEL-33; HEL-S-72p; HSC54; HSC70; HSC71; Hsc73; HSP70; HSP71; HSP73; HSPA10; HSPA8; I79_022974; LAP-1; LAP1; NIP71
ATP binding
ATPase activity, coupled
unfolded protein binding
G-protein coupled receptor binding
phosphatidylserine binding
protein binding
ATPase activity
enzyme binding
MHC class II protein complex binding
heat shock protein binding
ubiquitin protein ligase binding
poly(A) RNA binding
C3HC4-type RING finger domain binding
cadherin binding involved in cell-cell adhesion
receptor binding
ADP binding
nucleotide binding
transcription, DNA-templated
mRNA processing
protein folding
RNA splicing
protein refolding
negative regulation of transcription, DNA-templated
positive regulation of mRNA splicing, via spliceosome
chaperone mediated protein folding requiring cofactor
regulation of cell cycle
chaperone-mediated protein folding
clathrin coat disassembly
negative regulation of fibril organization
response to stress
mRNA splicing, via spliceosome
protein targeting to lysosome
response to unfolded protein
neurotransmitter secretion
cellular response to starvation
viral process
regulation of protein stability
regulation of protein complex assembly
regulation of mRNA stability
ATP metabolic process
regulation of protein complex stability
chaperone-mediated autophagy
late endosomal microautophagy
protein targeting to lysosome involved in chaperone-mediated autophagy
chaperone-mediated protein transport involved in chaperone-mediated autophagy
cell-cell adhesion
regulation of cellular response to heat
regulation of protein import
chaperone-mediated autophagy translocation complex disassembly
regulation of transcription, DNA-templated
post-Golgi vesicle-mediated transport
membrane organization
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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