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Invitrogen
HSD11B2 Polyclonal Antibody
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FIGURE: 1 / 2
HSD11B2 Antibody (PA5-96923) in WB
Western blot was performed using Anti-HSD11B2 Polyclonal Antibody (Product # PA5-96923), and 40 kDa band corresponding to HSD11B2 was observed in BeWo cell line on Forskolin treatment and in LNCaP cell line. Additional uncharacterized bands (*) were observed across the cell lines. Whole cell extracts (30 µg lysate) of BeWo (Lane 1), BeWo treated with Forskolin (100 µM for 24hr) (Lane 2), and LNCaP (Lane 3) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a... View More
Product Details
PA5-96923
Product Specifications
Species Reactivity
Human,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 266-405 of human HSD11B2 (NP_0001873)
Conjugate
Form
Liquid
Concentration
0.48 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2808725
Product Specific Information
Immunogen sequence: KTESVRNVGQ WEKRKQLLLA NLPQELLQAY GKDYIEHLHG QFLHSLRLAM SDLTPVVDAI TDALLAARPR RRYYPGQGLG LMYFIHYYLP EGLRRRFLQA FFISHCLPRA LQPGQPGTTP PQDAAQDPNL SPGPSPAVAR; Positive Samples: HepG2; Cellular Location: Endoplasmic reticulum, Microsome
Target Information
There are at least two isozymes of the corticosteroid 11-beta-dehydrogenase, a microsomal enzyme complex responsible for the interconversion of cortisol and cortisone. The type I isozyme has both 11-beta-dehydrogenase (cortisol to cortisone) and 11-oxoreductase (cortisone to cortisol) activities. The type II isozyme, encoded by this gene, has only 11-beta-dehydrogenase activity. In aldosterone-selective epithelial tissues such as the kidney, the type II isozyme catalyzes the glucocorticoid cortisol to the inactive metabolite cortisone, thus preventing illicit activation of the mineralocorticoid receptor. In tissues that do not express the mineralocorticoid receptor, such as the placenta and testis, it protects cells from the growth-inhibiting and/or pro-apoptotic effects of cortisol, particularly during embryonic development. Mutations in this gene cause the syndrome of apparent mineralocorticoid excess and hypertension.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: -HSD11 type II; 11-beta-HSD type II; 11-beta-HSD2; 11-beta-hydroxysteroid dehydrogenase type 2; 11-beta-hydroxysteroid dehydrogenase type II; 11-DH2; 11-HSD type II; Corticosteroid 11-beta-dehydrogenase isozyme 2; hydroxysteroid (11-beta) dehydrogenase 2; Hydroxysteroid dehydrogenase, 11 beta type 2; NAD-dependent 11-beta-hydroxysteroid dehydrogenase; Short chain dehydrogenase/reductase family 9C member 3
Gene Aliases: AME; AME1; HSD11B2; HSD11K; HSD2; SDR9C3
UniProt ID: (Human) P80365, (Rat) P50233
Entrez Gene ID: (Human) 3291, (Rat) 25117
response to hypoxia
regulation of blood volume by renal aldosterone
glucocorticoid biosynthetic process
female pregnancy
response to food
response to insulin
response to drug
response to glucocorticoid
oxidation-reduction process
metabolic process
glucocorticoid metabolic process
response to steroid hormone
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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