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Invitrogen
HSD17B7 Polyclonal Antibody
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FIGURE: 1 / 6
HSD17B7 Antibody (PA5-143945) in ICC/IF
Immunocytochemistry analysis of HSD17B7 in HEPG2 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. Samples were then incubated in HSD17B7 Polyclonal antibody (Product # PA5-143945) using a dilution of 5 μg/mL. Cy3 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for ... View More
Product Details
PA5-143945
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived human HSD17B7 recombinant protein (Position: L16-L336).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_3075159
Product Specific Information
Adding 0.2 mL of distilled water will yield a concentration of 500 µg/mL.
Positive Control - WB: human HepG2 whole cell, human HL-60 whole cell, human 293T whole cell, human Jurkat whole cell, human RT4 whole cell, rat liver tissue, rat lung tissue, rat RH35 whole cell. IHC: human liver cancer tissue, human prostate cancer tissue, human colorectal adenocarcinoma tissue. ICC/IF: HepG2 cell. Flow: JK cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Target Information
Responsible for the reduction of the keto group on the C-3 of sterols.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 17 beta-hydroxysteroid dehydrogenase type VII; 17-beta-HSD 7; 17-beta-hydroxysteroid dehydrogenase 7; 17beta hydroxysteroid dehydrogenase; 3-keto-steroid reductase; 3-keto-steroid reductase/17-beta-hydroxysteroid dehydrogenase 7; Dihydrotestosterone oxidoreductase; Estradiol 17-beta-dehydrogenase 7; hydroxysteroid (17-beta) dehydrogenase 7; hydroxysteroid dehydrogenase 17 beta, type 7; PRAP; PRL receptor-associated protein; Short chain dehydrogenase/reductase family 37C member 1; short chain dehydrogenase/reductase family 37C, member 1
Gene Aliases: 17HSD7; HSD17B7; PRAP; SDR37C1; UNQ2563/PRO6243
UniProt ID: (Human) P56937, (Rat) Q62904
Entrez Gene ID: (Human) 51478, (Rat) 29540
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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