Proteintech

HSF1 Polyclonal Antibody

HSF1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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HSF1 Antibody (16107-1-AP) in IHC (P)

Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using (Product # 16107-1-AP) (HSF1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Product Details

16107-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 9 publications 9 publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

HSF1 Fusion Protein Ag9023 (184-529 aa encoded by BC014638)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.21 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: KVVNKLIQF LISLVQSNRI LGVKRKIPLM LNDSGSAHSM PKYSRQFSLE HVHGSGPYSA PSPAYSSSSL YAPDAVASSG PIISDITELA PASPMASPGG SIDERPLSSS PLVRVKEEPP SPPQSPRVEE ASPGRPSSVD TLLSPTALID SILRESEPAP ASVTALTDAR GHTDTEGRPP SPPPTSTPEK CLSVACLDKN ELSDHLDAMD SNLDNLQTML SSHGFSVDTS ALLDLFSPSV TVPDMSLPDL DSSLASIQEL LSPQEPPRPP EAENSSPDSG KQLVHYTAQP LFLLDPGSVD TGSNDLPVLF ELGEGSYFSE GDGFAEDPTI SLLTGSEPPK AKDPTVS (184-529 aa encoded by BC014638 )

Target Information

All organisms respond to elevated temperatures and a variety of environmental stresses by rapid synthesis of heat shock RNAs and proteins. The regulation of heat shock gene transcription is mediated by the transcriptional activator, heat shock factor (HSF), which binds to heat shock response elements (HSEs). These HSEs are found as three repeats of a 5-nucleotide {nGAAn} module, arranged in alternating orientation and present upstream of all heat shock genes. The HSEs are highly conserved among species yet HSF purified from yeast, Drosophila and human have different molecular weights and the proteins do not show significant immunological cross reaction. Two HSFs have been identified in human cells, HSF1 and HSF2, which bind to the same HSEs and have 38% sequence identity. These factors are activated by distinct stimuli, HSF1 is responsive to classical stress signals such as heat, heavy metals and oxidative reagents, whereas HSF2 is activated during hemin-mediated differentiation of human erythroleukemia cells. HSF1 exists constitutively in the cytoplasm and the nucleus of unstressed cells as a monomer which lacks DNA binding activity. Through an unknown signal generated during stress, HSF1 becomes activated to a nuclear localized, trimeric state which binds to DNA. The phosphorylation of HSF1 is necessary for maximal transcription of heat shock genes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Cancer-associated fibroblasts suppressed ferroptosis in glioblastoma via upregulating lncRNA DLEU1.

American journal of physiology. Cell physiology

Zhao J,Yang S,Lv C,Liu Y

Mon May 01 00:00:00 UTC 2023

HSF1 Alleviates Brain Injury by Inhibiting NLRP3-Induced Pyroptosis in a Sepsis Model.

Mediators of inflammation

He YF,Hu XM,Khan MA,Yu BY,Sheng YC,Xiao XZ,Wan XX,Tan SP,Xiong K

Thu Feb 09 00:00:00 UTC 2023

Aspirin relieves the calcification of aortic smooth muscle cells by enhancing the heat shock response.

Pharmaceutical biology

Shen Q,Chen Q,Liu Y,Xue X,Shen X,He Q,Wang G,Han F

Thu Dec 01 00:00:00 UTC 2022

Skeletal muscle HSF1 prevents insulin resistance by improving glucose utilization.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Li Y,Lin S,Xu X,Jin W,Su Y,Yuan F,Zhang Y,Li Z,Zhou Y,Zhu L,Zhang L

Thu Dec 01 00:00:00 UTC 2022

Virtual screening based identification of miltefosine and octenidine as inhibitors of heat shock protein 90.

Naunyn-Schmiedeberg's archives of pharmacology

Li L,Yang M,Li C,Liu Y

Mon Nov 01 00:00:00 UTC 2021

RBM45 associates with nuclear stress bodies and forms nuclear inclusions during chronic cellular stress and in neurodegenerative diseases.

Acta neuropathologica communications

Collins M,Li Y,Bowser R

Fri Jun 26 00:00:00 UTC 2020

Duzhong Butiansu Prescription Improves Heat Stress-Induced Spermatogenic Dysfunction by Regulating Sperm Formation and Heat Stress Pathway.

Evidence-based complementary and alternative medicine : eCAM

Hou Y,Yuan P,Fu Y,Zhang Q,Wei Y,Gao L,Liu L,Wang X,Zheng X,Feng W

Wed Apr 13 00:00:00 UTC 2022

The Capsid Protein VP1 of Coxsackievirus B Induces Cell Cycle Arrest by Up-Regulating Heat Shock Protein 70.

Frontiers in microbiology

Wang Y,Zhao S,Chen Y,Wang T,Dong C,Wo X,Zhang J,Dong Y,Xu W,Feng X,Qu C,Wang Y,Zhong Z,Zhao W

Thu Oct 01 00:00:00 UTC 2020

Modification of the hTERT promoter by heat shock elements enhances the efficiency and specificity of cancer targeted gene therapy.

International journal of hyperthermia : the official journal of European Society for Hyperthermic Oncology, North American Hyperthermia Group

Wang X,Zhou P,Sun X,Wei G,Zhang L,Wang H,Yao J,Jia P,Zheng J

Sun May 01 00:00:00 UTC 2016

Modification of the hTERT promoter by heat shock elements enhances the efficiency and specificity of cancer targeted gene therapy.

International journal of hyperthermia : the official journal of European Society for Hyperthermic Oncology, North American Hyperthermia Group

Wang X,Zhou P,Sun X,Wei G,Zhang L,Wang H,Yao J,Jia P,Zheng J

Sun May 01 00:00:00 UTC 2016

Ginsenoside Rg3 induces FUT4-mediated apoptosis in H. pylori CagA-treated gastric cancer cells by regulating SP1 and HSF1 expressions.

Toxicology in vitro : an international journal published in association with BIBRA

Aziz F,Wang X,Liu J,Yan Q

Tue Mar 01 00:00:00 UTC 2016

Ginsenoside Rg3 induces FUT4-mediated apoptosis in H. pylori CagA-treated gastric cancer cells by regulating SP1 and HSF1 expressions.

Toxicology in vitro : an international journal published in association with BIBRA

Aziz F,Wang X,Liu J,Yan Q

Tue Mar 01 00:00:00 UTC 2016

Ginsenoside Rg3 induces FUT4-mediated apoptosis in H. pylori CagA-treated gastric cancer cells by regulating SP1 and HSF1 expressions.

Toxicology in vitro : an international journal published in association with BIBRA

Aziz F,Wang X,Liu J,Yan Q

Tue Mar 01 00:00:00 UTC 2016

Ginsenoside Rg3 induces FUT4-mediated apoptosis in H. pylori CagA-treated gastric cancer cells by regulating SP1 and HSF1 expressions.

Toxicology in vitro : an international journal published in association with BIBRA

Aziz F,Wang X,Liu J,Yan Q

Tue Mar 01 00:00:00 UTC 2016

Upregulation of Nuclear Heat Shock Factor 1 Contributes to Tumor Angiogenesis and Poor Survival in Patients With Non-Small Cell Lung Cancer.

The Annals of thoracic surgery

Cui J,Tian H,Chen G

Sat Aug 01 00:00:00 UTC 2015

Upregulation of Nuclear Heat Shock Factor 1 Contributes to Tumor Angiogenesis and Poor Survival in Patients With Non-Small Cell Lung Cancer.

The Annals of thoracic surgery

Cui J,Tian H,Chen G

Sat Aug 01 00:00:00 UTC 2015

Bioinformatics

Protein Aliases: Heat shock factor protein 1; Heat shock transcription factor 1; heat shock transcription factor 1 alpha isoform; heat shock transcription factor 1 beta isoform; heat shock transcription factor 1 gammaalpha isoform; heat shock transcription factor 1 gammabeta isoform; HSF 1; Hsf1gammaalpha; Hsf1gammabeta; HSTF 1

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Gene Aliases: AA960185; HSF1; Hsf1alpha; Hsf1beta; HSTF1

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UniProt ID: (Human) Q00613, (Mouse) P38532

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Entrez Gene ID: (Human) 3297, (Rat) 79245, (Mouse) 15499

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Function(s)

RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II core promoter sequence-specific DNA binding transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding RNA polymerase II intronic transcription regulatory region sequence-specific DNA binding chromatin binding transcription factor activity, sequence-specific DNA binding protein binding DNA binding sequence-specific DNA binding

Process(es)

negative regulation of transcription from RNA polymerase II promoter embryonic placenta development protein phosphorylation defense response female meiotic division spermatogenesis negative regulation of cell proliferation mRNA transcription response to lipopolysaccharide negative regulation of tumor necrosis factor production cellular response to heat positive regulation of multicellular organism growth positive regulation of transcription from RNA polymerase II promoter embryonic process involved in female pregnancy regulation of cellular response to heat in utero embryonic development transcription, DNA-templated regulation of transcription, DNA-templated response to heat transcription from RNA polymerase II promoter regulation of spindle checkpoint

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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HSF1 Polyclonal Antibody

HSF1 Antibody (16107-1-AP) in IHC (P)

Product Details

16107-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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