Invitrogen

HSF1 Polyclonal Antibody

3 Published Figures

21 References

View all (45) HSF1 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (4)
Published Figures (3)

HSF1 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 7

HSF1 Antibody (PA3-017) in ICC/IF

Immunofluorescent analysis of Heat Shock Factor 1 (HSF1) (green) in HeLa and NIH3T3 cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA (Product # 37525) for 15 minutes at room temperature. Cells were probed with a HSF1 polyclonal antibody (Product # PA3-017), at a dilution of 1:50 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat-anti rabbit IgG secondary antibody (Product # 35552) at a dilution of 1:400 ... View More

HSF1 Antibody in Immunoprecipitation (IP)

HSF1 Antibody in Western Blot, Immunocytochemistry, Flow Cytometry (WB, ICC/IF, Flow)

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Product Details

PA3-017

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:10,000

View 13 publications 13 publications

Immunocytochemistry (ICC/IF)

1:50

View 2 publications 2 publications

Flow Cytometry (Flow)

View 1 publication 1 publication

ELISA (ELISA)

Assay-dependent

Immunoprecipitation (IP)

2 µL

Neutralization (Neu)

View 1 publication 1 publication

Gel Shift (GS)

Assay-dependent

View 4 publications 4 publications

Product Specifications

Species Reactivity

C. elegans, Human, Mouse

Published species

Human, Mollusc, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant human HSF1 expressed in E. coli.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

PBS, whole serum

Contains

0.05% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_325968

Product Specific Information

PA3-017 detects heat shock factor 1 (HSF-1) from human, mouse and rat tissues and cells.

PA3-017 has been successfully used in Western blot, immunoprecipitation, immunofluorescence, ELISA, and gel super shift protocols. By Western blot, this antibody detects an ~83 kDa protein representing HSF-1 from NIH-3T3 cell lysate.

The PA3-017 immunogen is recombinant human HSF-1 protein expressed in E. coli.

Target Information

All organisms respond to elevated temperatures and a variety of environmental stresses by rapid synthesis of heat shock RNAs and proteins. The regulation of heat shock gene transcription is mediated by the transcriptional activator, heat shock factor (HSF), which binds to heat shock response elements (HSEs). These HSEs are found as three repeats of a 5-nucleotide {nGAAn} module, arranged in alternating orientation and present upstream of all heat shock genes. The HSEs are highly conserved among species yet HSF purified from yeast, Drosophila and human have different molecular weights and the proteins do not show significant immunological cross reaction. Two HSFs have been identified in human cells, HSF1 and HSF2, which bind to the same HSEs and have 38% sequence identity. These factors are activated by distinct stimuli, HSF1 is responsive to classical stress signals such as heat, heavy metals and oxidative reagents, whereas HSF2 is activated during hemin-mediated differentiation of human erythroleukemia cells. HSF1 exists constitutively in the cytoplasm and the nucleus of unstressed cells as a monomer which lacks DNA binding activity. Through an unknown signal generated during stress, HSF1 becomes activated to a nuclear localized, trimeric state which binds to DNA. The phosphorylation of HSF1 is necessary for maximal transcription of heat shock genes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Basic Limonoid modulates Chaperone-mediated Proteostasis and dissolve Tau fibrils.

Scientific reports

Gorantla NV,Das R,Chidambaram H,Dubey T,Mulani FA,Thulasiram HV,Chinnathambi S

PA3-017 was used in Western Blot to show that HSF1 in downstream increased the transcription of Hsp70 thus, aggravating cytosolic Hsp70 levels that can channel clearance of aberrant Tau.

Wed Mar 04 00:00:00 UTC 2020

Targeted production of reactive oxygen species in mitochondria to overcome cancer drug resistance.

Nature communications

Wang H,Gao Z,Liu X,Agarwal P,Zhao S,Conroy DW,Ji G,Yu J,Jaroniec CP,Liu Z,Lu X,Li X,He X

PA3-017 was used in Flow cytometry/Cell sorting to show that drug-laden LSC nanoparticles in combination with NIR laser treatment can effectively inhibit the growth of multidrug-resistant tumors in vivo.

Thu Feb 08 00:00:00 UTC 2018

Targeted production of reactive oxygen species in mitochondria to overcome cancer drug resistance.

Nature communications

Wang H,Gao Z,Liu X,Agarwal P,Zhao S,Conroy DW,Ji G,Yu J,Jaroniec CP,Liu Z,Lu X,Li X,He X

Thu Feb 08 00:00:00 UTC 2018

Targeted production of reactive oxygen species in mitochondria to overcome cancer drug resistance.

Nature communications

Wang H,Gao Z,Liu X,Agarwal P,Zhao S,Conroy DW,Ji G,Yu J,Jaroniec CP,Liu Z,Lu X,Li X,He X

Thu Feb 08 00:00:00 UTC 2018

Targeted production of reactive oxygen species in mitochondria to overcome cancer drug resistance.

Nature communications

Wang H,Gao Z,Liu X,Agarwal P,Zhao S,Conroy DW,Ji G,Yu J,Jaroniec CP,Liu Z,Lu X,Li X,He X

Thu Feb 08 00:00:00 UTC 2018

Response of small heat shock proteins in diabetic rat retina.

Investigative ophthalmology & visual science

Reddy VS,Raghu G,Reddy SS,Pasupulati AK,Suryanarayana P,Reddy GB

PA3-017 was used in western blot to study the expression of small heat shock proteins in the retina of diabetic rats

Tue Nov 19 00:00:00 UTC 2013

Heat shock protein accumulation and heat shock transcription factor activation in rat skeletal muscle during compensatory hypertrophy.

Acta physiologica (Oxford, England)

Locke M

PA3-017 was used in western blot to investigate the role of heat shock protein and heat shock transcription factor in compensatory hypertrophy of skeletal muscle

Sat Mar 01 00:00:00 UTC 2008

Cadmium-responsive element of the human heme oxygenase-1 gene mediates heat shock factor 1-dependent transcriptional activation.

The Journal of biological chemistry

Koizumi S,Gong P,Suzuki K,Murata M

PA3-017 was used in EMSA assay to investigate the role of CdRE in HSF-1 dependent transcriptional activation of ho-1 and hsp70

Fri Mar 23 00:00:00 UTC 2007

Castration inhibits exercise-induced accumulation of Hsp70 in male rodent hearts.

American journal of physiology. Heart and circulatory physiology

Milne KJ,Thorp DB,Melling CW,Noble EG

PA3-017 was used in western blot to investigate the role of castration during exercise-induced accumulation of Hsp70 in male rodent hearts

Sat Apr 01 00:00:00 UTC 2006

Phase I trial of 17-allylamino-17-demethoxygeldanamycin in patients with advanced cancer.

Journal of clinical oncology : official journal of the American Society of Clinical Oncology

Goetz MP,Toft D,Reid J,Ames M,Stensgard B,Safgren S,Adjei AA,Sloan J,Atherton P,Vasile V,Salazaar S,Adjei A,Croghan G,Erlichman C

PA3-017 was used in Western Blotting to characterise the maximum-tolerated dose, dose-limiting toxicities, efficacy, and pharmacokinetics of 17-allylamino-17-demethoxygeldanamycin in the treatment of advanced cancer.

Sun Feb 20 00:00:00 UTC 2005

Estrogen, heat shock proteins, and NFkappaB in human vascular endothelium.

Arteriosclerosis, thrombosis, and vascular biology

Hamilton KL,Mbai FN,Gupta S,Knowlton AA

PA3-017 was used in EMSA assay to study the relationship among estrogen treatment, NF kappa B activation and HSF-1 activation in human coronary artery endothelial cells

Wed Sep 01 00:00:00 UTC 2004

Exercise improves postischemic cardiac function in males but not females: consequences of a novel sex-specific heat shock protein 70 response.

Circulation research

Paroo Z,Haist JV,Karmazyn M,Noble EG

PA3-017 was used in western blot to investigate the relationship between exercise-mediated induction of Hsp70 and gender

Fri May 03 00:00:00 UTC 2002

Estrogen attenuates postexercise HSP70 expression in skeletal muscle.

American journal of physiology. Cell physiology

Paroo Z,Dipchand ES,Noble EG

PA3-017 was used in western blot to study the effect of estrogen on postexercise skeletal muscle HSP70 expression

Fri Feb 01 00:00:00 UTC 2002

Interspecific- and acclimation-induced variation in levels of heat-shock proteins 70 (hsp70) and 90 (hsp90) and heat-shock transcription factor-1 (HSF1) in congeneric marine snails (genus Tegula): implications for regulation of hsp gene expression.

The Journal of experimental biology

Tomanek L,Somero GN

PA3-017 was used in western blot to investigate heat-shock proteins in marine snails

Fri Mar 01 00:00:00 UTC 2002

Transcriptional activation domains of human heat shock factor 1 recruit human SWI/SNF.

Molecular and cellular biology

Sullivan EK,Weirich CS,Guyon JR,Sif S,Kingston RE

PA3-017 was used in western blot to investigate the interaction between heat shock transcription factor 1 and BRG1

Sat Sep 01 00:00:00 UTC 2001

Activation of HSF and selective increase in heat-shock proteins by acute dexamethasone treatment.

American journal of physiology. Heart and circulatory physiology

Sun L,Chang J,Kirchhoff SR,Knowlton AA

PA3-017 was used in EMSA assay to study the effects of the acute dexamethasone treatment on HSF and heat-shock proteins

Sat Apr 01 00:00:00 UTC 2000

The myocardial heat shock response following sodium salicylate treatment.

Cell stress & chaperones

Locke M,Atance J

PA3-017 was used in EMSA to investigate the effect of salicylate treatment on myocardial heat shock response

Sun Oct 01 00:00:00 UTC 2000

Higher induction of heat shock protein 72 by heat stress in cisplatin-resistant than in cisplatin-sensitive cancer cells.

Biochimica et biophysica acta

Abe T,Gotoh S,Higashi K

PA3-017 was used in immunocytochemistry to investigate the differential induction of heat shock protein 72 in cisplatin-resistant and cisplatin-sensitive cancer cells

Wed Apr 14 00:00:00 UTC 1999

Heat shock factor 1 mediates hemin-induced hsp70 gene transcription in K562 erythroleukemia cells.

The Journal of biological chemistry

Yoshima T,Yura T,Yanagi H

PA3-017 was used in western blot to study the role of the heat shock factor 1 during the hemin-induced hsp70 gene transcription

Fri Sep 25 00:00:00 UTC 1998

Heat shock factor 1 mediates hemin-induced hsp70 gene transcription in K562 erythroleukemia cells.

The Journal of biological chemistry

Yoshima T,Yura T,Yanagi H

PA3-017 was used in western blot to study the role of the heat shock factor 1 during the hemin-induced hsp70 gene transcription

Fri Sep 25 00:00:00 UTC 1998

Heat shock transcription factor 1 binds selectively in vitro to Ku protein and the catalytic subunit of the DNA-dependent protein kinase.

The Journal of biological chemistry

Huang J,Nueda A,Yoo S,Dynan WS

PA3-017 was used in blocking/activating experiment to study the interaction of heat shock transcription factor 1 with Ku protein and DNA-dependent protein kinase

Fri Oct 10 00:00:00 UTC 1997

Diminished heat shock response in the aged myocardium.

Cell stress & chaperones

Locke M,Tanguay RM

PA3-017 was used in western blot to examine the heat shock response in the myocardium from aged rats

Sun Dec 01 00:00:00 UTC 1996

Human heat shock factors 1 and 2 are differentially activated and can synergistically induce hsp70 gene transcription.

Molecular and cellular biology

Sistonen L,Sarge KD,Morimoto RI

PA3-017 was used in western blot to study the effect of heat shock factors 1 and 2 on hsp70 gene transcription

Tue Mar 01 00:00:00 UTC 1994

Bioinformatics

Protein Aliases: Heat shock factor protein 1; Heat shock transcription factor 1; heat shock transcription factor 1 alpha isoform; heat shock transcription factor 1 beta isoform; heat shock transcription factor 1 gammaalpha isoform; heat shock transcription factor 1 gammabeta isoform; HSF 1; Hsf1gammaalpha; Hsf1gammabeta; HSTF 1

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Gene Aliases: AA960185; HSF1; Hsf1alpha; Hsf1beta; HSTF1

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UniProt ID: (Human) Q00613, (Mouse) P38532

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Entrez Gene ID: (Human) 3297, (Mouse) 15499

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Function(s)

RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II core promoter sequence-specific DNA binding transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding RNA polymerase II intronic transcription regulatory region sequence-specific DNA binding chromatin binding transcription factor activity, sequence-specific DNA binding protein binding DNA binding sequence-specific DNA binding

Process(es)

negative regulation of transcription from RNA polymerase II promoter embryonic placenta development protein phosphorylation defense response female meiotic division spermatogenesis negative regulation of cell proliferation mRNA transcription response to lipopolysaccharide negative regulation of tumor necrosis factor production cellular response to heat positive regulation of multicellular organism growth positive regulation of transcription from RNA polymerase II promoter embryonic process involved in female pregnancy regulation of cellular response to heat in utero embryonic development transcription, DNA-templated regulation of transcription, DNA-templated transcription from RNA polymerase II promoter response to heat regulation of spindle checkpoint

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HSF1 Polyclonal Antibody

HSF1 Antibody (PA3-017) in ICC/IF

Product Details

PA3-017

Western Blot (WB)

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

ELISA (ELISA)

Immunoprecipitation (IP)

Neutralization (Neu)

Gel Shift (GS)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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