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Invitrogen
HSF1 Monoclonal Antibody (10H4), PerCP
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FIGURE: 1 / 3
HSF1 Antibody (MA5-45711) in ICC/IF
Immunocytochemistry/Immunofluorescence analysis using human heat shocked cervical cancer cells. Fixation involved 2% Formaldehyde for 20 min at RT. Samples were incubated with HSF1 monoclonal antibody (Product # MA5-45711) at 1:100 for 12 hours at 4°C, followed by R-PE Goat Anti-Rat (yellow) at 1:200 for 2 hours at RT. Counterstain used was DAPI (blue) nuclear stain at 1:40,000 for 2 hours at RT. Localization: Cytoplasm. Localizes to the nucleus upon activation. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-HSF1 antibody. (C... View More
Product Details
MA5-45711
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bovine,
Guinea pig,
Hamster,
Human,
Mouse,
Non-human primate,
Rabbit,
Rat
Host/Isotype
Rat
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
10H4
Immunogen
Purified recombinant mouse HSF1 protein
Conjugate
Excitation/Emission Max
482/675 nm
View spectra
Form
Liquid
Concentration
1 mg/mL
Purification
Protein G
Storage buffer
95.64mM phosphate/2.48mM MES, pH 7.4, with 0.5M EDTA
Contains
no preservative
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2932165
Product Specific Information
1 µg/mL of MA5-45711 was sufficient for detection of HSF1 in 20 µg of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Rabbit anti-rat IgG: AP as the secondary antibody.|Detects approximately 85kDa (unstressed cell lysates) and approximately 95kDa (heat shocked cell lysates).
Target Information
All organisms respond to elevated temperatures and a variety of environmental stresses by rapid synthesis of heat shock RNAs and proteins. The regulation of heat shock gene transcription is mediated by the transcriptional activator, heat shock factor (HSF), which binds to heat shock response elements (HSEs). These HSEs are found as three repeats of a 5-nucleotide {nGAAn} module, arranged in alternating orientation and present upstream of all heat shock genes. The HSEs are highly conserved among species yet HSF purified from yeast, Drosophila and human have different molecular weights and the proteins do not show significant immunological cross reaction. Two HSFs have been identified in human cells, HSF1 and HSF2, which bind to the same HSEs and have 38% sequence identity. These factors are activated by distinct stimuli, HSF1 is responsive to classical stress signals such as heat, heavy metals and oxidative reagents, whereas HSF2 is activated during hemin-mediated differentiation of human erythroleukemia cells. HSF1 exists constitutively in the cytoplasm and the nucleus of unstressed cells as a monomer which lacks DNA binding activity. Through an unknown signal generated during stress, HSF1 becomes activated to a nuclear localized, trimeric state which binds to DNA. The phosphorylation of HSF1 is necessary for maximal transcription of heat shock genes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: heat shock factor 1; Heat shock factor protein 1; Heat shock transcription factor 1; heat shock transcription factor 1 alpha isoform; heat shock transcription factor 1 beta isoform; heat shock transcription factor 1 gammaalpha isoform; heat shock transcription factor 1 gammabeta isoform; HSF 1; Hsf1gammaalpha; Hsf1gammabeta; HSTF 1
Gene Aliases: AA960185; BOS_13649; HSF1; Hsf1alpha; Hsf1beta; HSTF1
UniProt ID: (Bovine) Q08DJ8, (Human) Q00613, (Mouse) P38532
Entrez Gene ID: (Bovine) 506235, (Rabbit) 100350887, (Human) 3297, (Chinese hamster) 100762942, (Rat) 79245, (Mouse) 15499
RNA polymerase II intronic transcription regulatory region sequence-specific DNA binding
chromatin binding
transcription factor activity, sequence-specific DNA binding
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II core promoter sequence-specific DNA binding
transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding
protein binding
DNA binding
sequence-specific DNA binding
embryonic placenta development
transcription, DNA-templated
protein phosphorylation
female meiotic division
spermatogenesis
negative regulation of cell proliferation
response to lipopolysaccharide
negative regulation of tumor necrosis factor production
cellular response to heat
positive regulation of multicellular organism growth
positive regulation of transcription from RNA polymerase II promoter
embryonic process involved in female pregnancy
regulation of spindle checkpoint
negative regulation of transcription from RNA polymerase II promoter
defense response
mRNA transcription
regulation of cellular response to heat
in utero embryonic development
regulation of transcription, DNA-templated
response to heat
transcription from RNA polymerase II promoter
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