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HSP20 Antibody (PA5-89649) in WB
Western blot analysis of HSP20 in extracts of various cell lines. Samples were incubated with HSP20 Polyclonal antibody (Product # PA5-89649) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.
Product Details
PA5-89649
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-160 of human HSP20/HSP20/HSPB6 (NP_6532181)
Conjugate
Form
Liquid
Concentration
0.68 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2805709
Product Specific Information
Immunogen sequence: MEIPVPVQPS WLRRASAPLP GLSAPGRLFD QRFGEGLLEA ELAALCPTTL APYYLRAPSV ALPVAQVPTD PGHFSVLLDV KHFSPEEIAV KVVGEHVEVH ARHEERPDEH GFVAREFHRR YRLPPGVDPA AVTSALSPEG VLSIQAAPAS AQAPPPAAAK; Positive Samples: A375, Mouse heart, Rat skeletal muscle; Cellular Location: Cytoplasm, Nucleus
Target Information
Heat Shock Protein 20 (HSP20), encoded by the HSPB6 gene, is a member of the small heat shock protein (sHSP) family, prominently involved in cellular stress response and maintenance of cytoskeletal integrity. HSP20 is ubiquitously expressed across various tissues, including cardiac and smooth muscle, where its roles are vital in modulating stress resilience and muscle function. It exerts protective effects against apoptosis and oxidative stress by interacting with cell signaling pathways that enhance cellular survival and protein stabilization. In cardiac muscle, HSP20 is known for its cardioprotective actions during ischemic injury, helping preserve myocardial function by inhibiting hypertrophic signaling pathways and promoting muscle relaxation. Studies highlight its involvement in vasodilation processes, as HSP20 interacts with myosin to regulate smooth muscle contraction and dilatation. Beyond its role in muscle physiology, HSP20 has been investigated for its potential therapeutic applications in cardiovascular diseases and metabolic disorders, where its expression and function could be pivotal in disease modulation and treatment strategies.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: cardiac heat shock protein p20; cardiac p20; epididymis luminal protein 55; Heat shock 20 kDa-like protein p20; heat shock 20-kDa protein; Heat shock protein; Heat shock protein beta-6; heat shock protein family B (small) member B6; Heat shock protein family B member 6; heat shock protein, alpha-crystallin-related, B6; HSP; Hsp20; HspB6; protein phosphatase 1, regulatory subunit 91
Gene Aliases: AA387366; Gm479; HEL55; Hsp20; HSPB6; p20; PPP1R91
UniProt ID: (Human) O14558, (Rat) P97541, (Mouse) Q5EBG6
Entrez Gene ID: (Human) 126393, (Rat) 192245, (Mouse) 243912
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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