Invitrogen

HSP27 Polyclonal Antibody

HSP27 Antibody in Immunocytochemistry (ICC/IF)

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HSP27 Antibody (PA1-017) in ICC/IF

Immunofluorescent analysis of Heat Shock Protein 27 (HSP27) (green) in HeLa cells and negative control NIH3T3 cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA (Product # 37525) for 15 minutes at room temperature. Cells were probed with HSP27 polyclonal antibody (Product # PA1-017), at a dilution of 1:50 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat-anti-rabbit IgG secondary antibody (Product # 35552... View More

HSP27 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

HSP27 Antibody in Western Blot, Immunoprecipitation (WB, IP)

Product Image Gallery

HSP27 Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell lines owing to their inherent genetic constitution. Relative expression of HSP27 was observed in MCF-7, MDA-MB-231, PC-3, DU 145, HeLa and LNCaP in comparison to HT-1080 using Anti-HSP27 Polyclonal Antibody (Product # PA1-017) in Western Blot. {RE}

Product Details

PA1-017

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:2,000

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

4 µg/mL

Immunocytochemistry (ICC/IF)

Assay-dependent

Immunoprecipitation (IP)

View 1 publication 1 publication

Miscellaneous PubMed (MISC)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Non-human primate, Rat

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to residues L(10) L R G P S W D P F R C(21) of human Heat Shock Protein 27.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2120942

Product Specific Information

PA1-017 detects heat shock protein 27(hsp27) from rat, mouse, monkey, and human tissue tissues.

PA1-017 has been successfully used in Western blot, immunofluorescence, immunocytochemistry, and immunohistochemistry procedures. By Western blot, this antibody detects a ~27 kDa protein which corresponds to hsp27 from HeLa cells. Immunocytochemical staining of hsp27 in HeLa cells with this antibody results in diffuse cytoplasmic staining before heat shock and diffuse cytoplasmic along with punctate staining patterns after.

The PA1-017 immunogen is a synthetic peptide corresponding to residues L(10) L R G P S W D P F R C(21) of human Heat Shock Protein 27. This peptide is 81% conserved in mice, rats, and hamsters. The phosphorylated peptide (Cat. # PEP-187) and non-phosphorylated peptide (Cat. # PEP-240) are available for use in neutralization and control experiments.

Target Information

In response to adverse changes in their environment, cells from many organisms increase the expression of a class of proteins referred to as heat shock or stress proteins. HSPB1 (heat shock protein beta-1 or HSP27) is a small heat shock protein which functions as a molecular chaperone that maintains denatured proteins in a folding-competent state. It plays a role in stress resistance and actin organization. Through its molecular chaperone activity, HSP27 regulates numerous biological processes including the phosphorylation and the axonal transport of neurofilament proteins. Mutations in the gene can result in Charcot-Marie-Tooth disease 2F and Neuronopathy distal hereditary motor 2B.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

The Glyoxalase System Is a Novel Cargo of Amniotic Fluid Stem-Cell-Derived Extracellular Vesicles.

Antioxidants (Basel, Switzerland)

Romani R,Talesa VN,Antognelli C

PA1-017 was used in Western Blotting and Immunoprecipitaiton to investigate whether MG-derived MG-H1, as well as glyoxalases, could be novel molecule cargo in extracellular vesicles.

Fri Aug 05 00:00:00 UTC 2022

The Glyoxalase System Is a Novel Cargo of Amniotic Fluid Stem-Cell-Derived Extracellular Vesicles.

Antioxidants (Basel, Switzerland)

Romani R,Talesa VN,Antognelli C

PA1-017 was used in Western Blotting and Immunoprecipitaiton to investigate whether MG-derived MG-H1, as well as glyoxalases, could be novel molecule cargo in extracellular vesicles.

Fri Aug 05 00:00:00 UTC 2022

The Glyoxalase System Is a Novel Cargo of Amniotic Fluid Stem-Cell-Derived Extracellular Vesicles.

Antioxidants (Basel, Switzerland)

Romani R,Talesa VN,Antognelli C

PA1-017 was used in Western Blotting and Immunoprecipitaiton to investigate whether MG-derived MG-H1, as well as glyoxalases, could be novel molecule cargo in extracellular vesicles.

Fri Aug 05 00:00:00 UTC 2022

Heat Shock Protein A6 Is Especially Involved in Enterovirus 71 Infection.

Frontiers in microbiology

Jia J,Liu G,Zhong J,Yan R,Song X,Zheng K,Ren Z,He Z,Zhu Q

Published figure using HSP27 polyclonal antibody (Product # PA1-017) in Western Blot

Tue Mar 22 00:00:00 UTC 2022

Small heat shock protein 27 (Hsp27) expression is highly induced in rat myometrium during late pregnancy and labour.

Reproduction (Cambridge, England)

White BG,Williams SJ,Highmore K,Macphee DJ

PA1-017 was used in immunohistochemistry and western blot to examine the expression of Hsp27 in rat myometrium during late pregnancy and labour

Sat Jan 01 00:00:00 UTC 2005

Bioinformatics

Protein Aliases: 28 kDa heat shock protein; DKFZp586P1322; epididymis secretory protein Li 102; Estrogen-regulated 24 kDa protein; Heat shock 27 kDa protein; heat shock 27kD protein 1; heat shock 27kDa protein 1; Heat shock protein; Heat shock protein beta-1; Heat shock protein family B member 1; HS; HSP; HSP 27; HspB1; Phospho-HSP 27; SRP27; Stress-responsive protein 27

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Gene Aliases: CMT2F; HEL-S-102; HMN2B; HS.76067; Hsp25; HSP27; HSP28; HSPB1; SRP27

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UniProt ID: (Human) P04792

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Entrez Gene ID: (Human) 3315, (Rat) 24471

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Function(s)

protein kinase C binding protein binding protein kinase C inhibitor activity protein kinase binding identical protein binding ubiquitin binding poly(A) RNA binding

Process(es)

retina homeostasis regulation of translational initiation negative regulation of protein kinase activity movement of cell or subcellular component response to unfolded protein response to virus regulation of autophagy positive regulation of interleukin-1 beta production intracellular signal transduction cellular response to vascular endothelial growth factor stimulus positive regulation of endothelial cell chemotaxis by VEGF-activated vascular endothelial growth factor receptor signaling pathway positive regulation of tumor necrosis factor biosynthetic process negative regulation of apoptotic process regulation of I-kappaB kinase/NF-kappaB signaling regulation of mRNA stability positive regulation of blood vessel endothelial cell migration positive regulation of angiogenesis vascular endothelial growth factor receptor signaling pathway platelet aggregation negative regulation of protein serine/threonine kinase activity negative regulation of oxidative stress-induced intrinsic apoptotic signaling pathway positive regulation of endothelial cell chemotaxis

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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HSP27 Polyclonal Antibody

HSP27 Antibody (PA1-017) in ICC/IF

Product Details

PA1-017

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Miscellaneous PubMed (MISC)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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