Invitrogen

HSP27 Monoclonal Antibody (STRSN), eBioscience™

HSP27 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 10

HSP27 Antibody (14-9112-80) in ICC/IF

Immunofluorescence analysis of HSP27 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with HSP27 Monoclonal Antibody (STRSN), eBioscience™ (Product # 14-9112-80) at 2.5 µg/mL in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 ... View More

HSP27 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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HSP27 Antibody — Antibody specificity was demonstrated by CRISPR-Cas9 mediated knockout of target protein. A reduced signal was observed for target protein in HeLa Cas9 cell line transduced with HSP27 Lentiviral sgRNA compared to control cell line using Anti-HSP27 Monoclonal Antibody (STRSN), eBioscience™ (Product # 14-9112-80). {KO}

Product Details

14-9112-80

Applications

Tested Dilution

Publications

Western Blot (WB)

1 µg/mL

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1 µg/mL

Immunocytochemistry (ICC/IF)

1-2.5 µg/mL

ELISA (ELISA)

Assay-Dependent

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

STRSN

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_11219272

Product Specific Information

Description: The monoclonal antibody STRSN recognizes human heat shock protein 27 (HSP27). The heat shock proteins are a large family of proteins that are induced in response to stressors such as changing temperatures, cytokine levels, and presence of various chemicals. Heat shock proteins serve as molecular chaperones, binding to other proteins to maintain proper protein structure or by folding denatured proteins and preventing activation of caspases that could lead to apoptosis. Unlike the larger HSP proteins, HSP27 belongs to a unique subgroup that is involved in apoptosis regulation. Expression of HSP27 is constitutive, but upon stress HSP27 is upregulated and can relocate from the cytoplasm to the nucleus. Increased HSP27 expression with certain carcinomas has been observed. In breast cancer, HSP27 expression has been shown to be responsive to estrogen levels. In both breast cancer and melanoma, high levels of HSP27 correlate with more aggressive tumors and decreased survival. Increased levels of HSP27 in the serum of patients with Chronic Obstructive Pulmonary Disease (COPD), breast cancer and melanoma may serve as a potential diagnostic marker. No crossreactivity is observed with HSP70, 90, 104 or 110.

Applications Reported: This STRSN antibody has been reported for use in western blotting, immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, ELISA, and immunocytochemistry.

Applications Tested: This STRSN antibody has been tested by western blot of reduced HeLa cell lysate, immunocytochemistry on fixed and permeabilized A549 cells, and by immunohistochemistry on formalin-fixed paraffin embedded (FFPE) human tissue with either low or high pH antigen retreival at less than or equal to 1 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

In response to adverse changes in their environment, cells from many organisms increase the expression of a class of proteins referred to as heat shock or stress proteins. HSPB1 (heat shock protein beta-1 or HSP27) is a small heat shock protein which functions as a molecular chaperone that maintains denatured proteins in a folding-competent state. It plays a role in stress resistance and actin organization. Through its molecular chaperone activity, HSP27 regulates numerous biological processes including the phosphorylation and the axonal transport of neurofilament proteins. Mutations in the gene can result in Charcot-Marie-Tooth disease 2F and Neuronopathy distal hereditary motor 2B.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Suppression of heat shock protein 27 induces long-term dormancy in human breast cancer.

Proceedings of the National Academy of Sciences of the United States of America

Straume O,Shimamura T,Lampa MJ,Carretero J,Øyan AM,Jia D,Borgman CL,Soucheray M,Downing SR,Short SM,Kang SY,Wang S,Chen L,Collett K,Bachmann I,Wong KK,Shapiro GI,Kalland KH,Folkman J,Watnick RS,Akslen LA,Naumov GN

Published figure using HSP27 monoclonal antibody (Product # 14-9112-82) in Western Blot

Tue May 29 00:00:00 UTC 2012

Bioinformatics

Protein Aliases: 28 kDa heat shock protein; DKFZp586P1322; epididymis secretory protein Li 102; Estrogen-regulated 24 kDa protein; Heat shock 27 kDa protein; heat shock 27kD protein 1; heat shock 27kDa protein 1; Heat shock protein; Heat shock protein beta-1; Heat shock protein family B member 1; HS; HSP; HSP 27; HspB1; Phospho-HSP 27; SRP27; Stress-responsive protein 27

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Gene Aliases: CMT2F; HEL-S-102; HMN2B; HS.76067; Hsp25; HSP27; HSP28; HSPB1; SRP27

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UniProt ID: (Human) P04792

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Entrez Gene ID: (Human) 3315

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Function(s)

protein kinase C binding protein binding protein kinase C inhibitor activity protein kinase binding identical protein binding ubiquitin binding poly(A) RNA binding

Process(es)

retina homeostasis regulation of translational initiation negative regulation of protein kinase activity movement of cell or subcellular component response to unfolded protein response to virus regulation of autophagy positive regulation of interleukin-1 beta production intracellular signal transduction cellular response to vascular endothelial growth factor stimulus positive regulation of endothelial cell chemotaxis by VEGF-activated vascular endothelial growth factor receptor signaling pathway positive regulation of tumor necrosis factor biosynthetic process negative regulation of apoptotic process regulation of I-kappaB kinase/NF-kappaB signaling regulation of mRNA stability positive regulation of blood vessel endothelial cell migration positive regulation of angiogenesis vascular endothelial growth factor receptor signaling pathway platelet aggregation negative regulation of protein serine/threonine kinase activity negative regulation of oxidative stress-induced intrinsic apoptotic signaling pathway positive regulation of endothelial cell chemotaxis

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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HSP27 Monoclonal Antibody (STRSN), eBioscience™

HSP27 Antibody (14-9112-80) in ICC/IF

Product Details

14-9112-80

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

ELISA (ELISA)

Species Reactivity

Host/Isotype

Class

Type

Clone

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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