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Invitrogen
HSP40 Polyclonal Antibody
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FIGURE: 1 / 8
HSP40 Antibody (PA5-17382) in ICC/IF
Immunofluorescence analysis of HSP40 was performed using 70% confluent log phase HeLa cells heat shocked at 43°C for 1 hr. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with HSP40 Polyclonal Antibody (Product # PA5-17382) at 1:400 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® ... View More
Product Details
PA5-17382
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Non-human primate,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide corresponding to Glu300 of human HSP40/Hdj1
Conjugate
Form
Liquid
Concentration
35 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_10984351
Product Specific Information
It is not recommended to aliquot this antibody.
This antibody is not cross-reactive with other HSPs.
Target Information
TCF-9 (transcription factor 9), also known as C2orf3 (chromosome 2 open reading frame 3), GCF (GC-rich sequence DNA-binding factor) or DNABF, is a 781 amino acid nuclear protein that belongs to the GCF family. Widely expressed, GCF binds the GC-rich sequences of beta-Actin, EGFR and calcium-dependent protease (CANP) promoters. GCF contains multiple phosphoserine and phosphothreonine residues, and two GCF isoforms are produced due to alternative splicing events. GCF is considered a candidate for susceptibility to dyslexia (DYX3) as both genes reside in close proximity on human chromosome 2. Chromosome 2 is the second largest human chromosome and consists of 237 million bases, encodes over 1,400 genes and makes up approximately 8% of the human genome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: DnaJ (Hsp40) homolog, subfamily B, member 1; DnaJ homolog subfamily B member 1; DnaJ protein homolog 1; hDj-1; Heat shock 40 kDa protein 1; Heat shock protein; HSP; HSP 40; HSP40; Human DnaJ protein 1; NDAJB1; radial spoke 16 homolog B
Gene Aliases: 0610007I11Rik; DjB1; DNAJ1; DNAJB1; HDJ1; Hsp40; HSPF1; RSPH16B; Sis1
UniProt ID: (Human) P25685, (Mouse) Q9QYJ3
Entrez Gene ID: (Human) 3337, (Rat) 361384, (Mouse) 81489
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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