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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • SERPINH1 Antibodies

          Proteintech

          HSP47 Polyclonal Antibody

          13 References
          View all (28) SERPINH1 antibodies

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          Datasheet
          Protocols
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          Datasheet
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          Cite HSP47 Polyclonal Antibody

          • Antibody Testing Data (9)
          HSP47 Antibody in Immunocytochemistry (ICC/IF)
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          HSP47 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 9

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          HSP47 Antibody (10875-1-AP) in ICC/IF

          Immunofluorescent analysis of HeLa cells using 10875-1-AP (HSP47 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L). {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          HSP47 Antibody in Immunocytochemistry (ICC/IF)
          HSP47 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          HSP47 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          HSP47 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          HSP47 Antibody in Western Blot (WB)
          HSP47 Antibody in Western Blot (WB)
          HSP47 Antibody in Western Blot (WB)
          HSP47 Antibody in Western Blot (WB)
          HSP47 Antibody in Flow Cytometry (Flow)

          Product Details

          10875-1-AP

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:500-1:1,000
          View 6 publications 6 publications

          Immunohistochemistry (IHC)

          -
          View 3 publications 3 publications

          Immunohistochemistry (Paraffin) (IHC (P))

          1:20-1:200
          -

          Immunocytochemistry (ICC/IF)

          1:10-1:100
          View 2 publications 2 publications

          Flow Cytometry (Flow)

          0.2 µg/1x10^6 cells
          -

          Immunoprecipitation (IP)

          -
          View 1 publication 1 publication

          Not applicable (N/A)

          -
          View 1 publication 1 publication
          Product Specifications

          Species Reactivity

          Human, Mouse

          Published species

          Human, Mouse

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          HSP47 Fusion Protein Ag1301 (1-418 aa encoded by BC014623)
          View immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          0.41 mg/mL

          Purification

          Antigen affinity chromatography

          Storage buffer

          PBS, pH 7.3, with 50% glycerol

          Contains

          0.02% sodium azide

          Storage conditions

          -20°C

          Shipping conditions

          Wet ice (domestic); Dry ice (international)

          Product Specific Information

          Immunogen sequence: MRSLLLLSA FCLLEAALAA EVKKPAAAAA PGTAEKLSPK AATLAERSAG LAFSLYQAMA KDQAVENILV SPVVVASSLG LVSLGGKATT ASQAKAVLSA EQLRDEEVHA GLGELLRSLS NSTARNVTWK LGSRLYGPSS VSFADDFVRS SKQHYNCEHS KINFRDKRSA LQSINEWAAQ TTDGKLPEVT KDVERTDGAL LVNAMFFKPH WDEKFHHKMV DNRGFMVTRS YTVGVMMMHR TGLYNYYDDE KEKLQIVEMP LAHKLSSLII LMPHHVEPLE RLEKLLTKEQ LKIWMGKMQK KAVAISLPKG VVEVTHDLQK HLAGLGLTEA IDKNKADLSR MSGKKDLYLA SVFHATAFEL DTDGNPFDQD IYGREELRSP KLFYADHPFI FLVRDTQSGS LLFIGRLVRP KGDKMRDEL (1-418 aa encoded by BC014623 )

          Target Information

          SERPINH1 is a member of the serpin superfamily of serine proteinase inhibitors, and its expression is induced by heat shock. The SERPINH1 protein localizes to the endoplasmic reticulum lumen and binds collagen; thus it is thought to be a molecular chaperone involved in the maturation of collagen molecules. Autoantibodies to SERPINH1 have been found in patients with rheumatoid arthritis.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: 47 kDa heat shock protein; Arsenic-transactivated protein 3; AsTP 3; AsTP3; beta enolase repressor factor 1; CBP 1; CBP 2; Cell proliferation-inducing gene 14 protein; collagen binding protein 1; Collagen-binding protein; Colligen; Colligin; Colligin 1; Colligin 2; colligin-1; colligin-2; HGNC 1547; Hsp 47; PIG 14; RA A47; rheumatoid arthritis antigen A-47; Rheumatoid arthritis-related antigen RA-A47; serine (or cysteine) proteinase inhibitor, clade H (heat shock protein 47), member 1; serine (or cysteine) proteinase inhibitor, clade H (heat shock protein 47), member 1, (collagen binding protein 1); serine (or cysteine) proteinase inhibitor, clade H (heat shock protein 47), member 2; serine (or cysteine) proteinase inhibitor, clade H (heat shock protein 47), member 2, (collagen-binding protein 2); Serine protease inhibitor J6; Serpin H1; serpin peptidase inhibitor, clade H (heat shock protein 47), member 1, (collagen binding protein 1)

          View more View less

          Gene Aliases: AsTP3; BERF-1; CBP1; CBP2; gp46; HSP47; J6; OI10; PIG14; PPROM; RA-A47; SERPINH1; SERPINH2

          View more View less

          UniProt ID: (Human) P50454, (Mouse) P19324

          View more View less

          Entrez Gene ID: (Human) 871, (Mouse) 12406

          View more View less

          Function(s)
          serine-type endopeptidase inhibitor activity collagen binding poly(A) RNA binding unfolded protein binding protein binding
          Process(es)
          chondrocyte development involved in endochondral bone morphogenesis negative regulation of endopeptidase activity collagen fibril organization collagen biosynthetic process protein maturation response to unfolded protein
          It has to be done as per old AB suggested Products section.
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