Invitrogen

HSP60 Monoclonal Antibody (LK2), FITC

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Datasheet
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Datasheet
Protocols
Questions & Answers

Product Details

MA5-45113

Applications
Tested Dilution
Publications

Western Blot (WB)

1:4,000
-

Immunohistochemistry (Paraffin) (IHC (P))

Assay-dependent
-

Immunohistochemistry (PFA fixed) (IHC (PFA))

1:100,000
-

Flow Cytometry (Flow)

Assay-dependent
-
Product Specifications

Species Reactivity

Bacteria, Bovine, Dog, Chicken, Fish, Guinea pig, Hamster, Human, Insect, Mouse, Non-human primate, Nematode, Plant, Pig, Rabbit, Rat, Yeast

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

LK2

Immunogen

Recombinant human HSP60
View immunogen

Conjugate

FITC FITC FITC

Excitation/Emission Max

498/517 nm View spectra spectra

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

9.1mM sodium bicarbonate/PBS with 640.91mM DMSO, 136.36mM ethanolamine

Contains

no preservative

Storage conditions

4°C, store in dark

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2931567

Product Specific Information

0.25 µg/mL of MA5-45113 was sufficient for detection of HSP60 in 10 µg of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using goat anti-mouse IgG as the secondary antibody.|Detects approximately 60kDa.

Target Information

This gene encodes a member of the chaperonin family. The encoded mitochondrial protein may function as a signaling molecule in the innate immune system. This protein is essential for the folding and assembly of newly imported proteins in the mitochondria. This gene is adjacent to a related family member and the region between the 2 genes functions as a bidirectional promoter. Several pseudogenes have been associated with this gene. Two transcript variants encoding the same protein have been identified for this gene. Mutations associated with this gene cause autosomal recessive spastic paraplegia 13.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: 60 kDa chaperonin; 60 kDa heat shock protein; 60 kDa heat shock protein, mitochondrial; chaperone ATPase HSP60; chaperonin; Chaperonin 60; CPN60; heat shock 60 kDa protein 1; heat shock 60kD protein 1 (chaperonin); heat shock 60kDa protein 1; heat shock 60kDa protein 1 (chaperonin); Heat shock protein; heat shock protein 1 (chaperonin); Heat shock protein 60; heat shock protein 60 (liver); Heat shock protein 60, mitochondrial; heat shock protein 65; Heat shock protein family D member 1; heat shock protein, 60 kDa; HSP; HSP-60; HSP-65; hsp60; Hsp60s1; HuCHA60; mitochondrial heat shock 60 kDa protein 1; Mitochondrial matrix protein P1; P1 protein; P60 lymphocyte protein; P66; short heat shock protein 60 Hsp60s1; Stimulator factor I 66 kDa component

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Gene Aliases: 60kDa; BOS_1963; CPN60; GROEL; HLD4; HSP-60; HSP60; HSP65; HSPD1; Hspd1-30p; HuCHA60; I79_011398; L8479.10; MIF4; MNA2; RCJMB04_7g5; SPG13; YLR259C

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UniProt ID: (Baker's yeast) P19882, (Chicken) Q5ZL72, (Human) P10809, (Chinese hamster) P18687, (Mouse) P63038, (Rat) P63039

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Entrez Gene ID: (Baker's yeast) 850963, (Dog) 478854, (Chicken) 424059, (Pig) 492279, (Human) 3329, (Bovine) 511913, (Rabbit) 100349912, (Chinese hamster) 100689473, (Mouse) 15510, (Rat) 63868

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Function(s)
nucleotide binding DNA replication origin binding single-stranded DNA binding ATP binding ATPase activity unfolded protein binding chaperone binding lipopolysaccharide binding p53 binding protein binding double-stranded RNA binding ubiquitin protein ligase binding poly(A) RNA binding protease binding protein complex binding insulin binding protein heterodimerization activity misfolded protein binding
Process(es)
B cell cytokine production MyD88-dependent toll-like receptor signaling pathway positive regulation of T cell mediated immune response to tumor cell activation of cysteine-type endopeptidase activity involved in apoptotic process response to unfolded protein positive regulation of interleukin-10 production positive regulation of interleukin-12 production positive regulation of interleukin-6 production protein refolding B cell proliferation positive regulation of macrophage activation positive regulation of apoptotic process negative regulation of apoptotic process isotype switching to IgG isotypes positive regulation of T cell activation protein folding 'de novo' protein folding response to stress cellular protein metabolic process protein import into mitochondrial intermembrane space protein stabilization chaperone-mediated protein complex assembly protein maturation detection of misfolded protein positive regulation of interferon-alpha production positive regulation of interferon-gamma production T cell activation B cell activation negative regulation of neuron apoptotic process positive regulation of inflammatory response chaperone mediated protein folding requiring cofactor response to cold viral process response to hypoxia response to heat response to organic substance response to organic cyclic compound response to lipopolysaccharide response to ATP response to cocaine response to drug response to hydrogen peroxide response to estrogen
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