Invitrogen

HSP70 Monoclonal Antibody (3A3)

HSP70 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 31

HSP70 Antibody (MA3-006) in ICC/IF

Immunofluorescent analysis of Heat Shock Protein 70 using Heat Shock Protein 70 Monoclonal antibody (3A3) (Product # MA3-006) shows staining in HeLa cells. Heat Shock Protein 70 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Heat Shock Protein 70 (Product # MA3-006) at a dilution of 1:100-1:200 over night at 4 °C, washed with PBS and incubate... View More

HSP70 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

HSP70 Antibody in Immunoprecipitation (IP)

HSP70 Antibody in Immunohistochemistry (IHC)

Product Image Gallery

HSP70 Antibody — Altered expression of proteins upon cell treatment demonstrates antibody specificity. Western blot using HSP70 Monoclonal Antibody (3A3) (Product # MA3-006), shows increased expression of proteins upon heat shock treatment. {TM}

Product Details

MA3-006

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:5,000

View 68 publications 68 publications

Immunohistochemistry (IHC)

View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:200

Immunocytochemistry (ICC/IF)

1:100

View 5 publications 5 publications

Flow Cytometry (Flow)

View 1 publication 1 publication

ELISA (ELISA)

View 3 publications 3 publications

Immunoprecipitation (IP)

2 µL

View 2 publications 2 publications

Neutralization (Neu)

View 1 publication 1 publication

Gel Shift (GS)

Assay-dependent

Miscellaneous PubMed (MISC)

View 2 publications 2 publications

Product Specifications

Species Reactivity

Amphibian, Arthropod, Avian, Dog, Fruit fly, Fish, Human, Mollusc, Mouse, Non-human primate, Plant, Pig, Rat, Yeast

Published species

Algae, Amphibian, Arthropod, Crab, Echinoderm, Fish, Fruit fly, Fungi, Human, Insect, Mollusc, Mouse, Non-human primate, Pig, Prawn, Rat, Yeast

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

3A3

Immunogen

Human recombinant HSP70 overexpressed in E. coli.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS

Contains

0.05% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_325454

Product Specific Information

MA3-006 detects several members of the heat shock protein 70 kDa (HSP70) gene family including HSP70, HSC70, p75, and following heat shock, HSP72 from yeast, Drosophila, fish, porcine, plant, mouse, avian, arthropod (red claw crayfish & black tiger prawn), amphibian, non-human primate, and human samples.

MA3-006 has been successfully used in Western blot, immunocytochemistry, immunofluorescence, gel shift, and immunoprecipitation procedures. By Western blot, this antibody detects proteins from ~70 kDa to ~78 kDa representing different members of the HSP70 family. 2-dimensional gel electrophoresis is required to resolve the heat induced form of these proteins from their constitutively expressed counterparts. Immunocytochemical staining of HSP70 in heat shocked HeLa cells with MA3-006 results in cytoplasmic staining.

The MA3-006 antigen is recombinant human HSP70 over-expressed in E. coli. Epitope mapping with a panel of HSP70 deletion mutants suggests that the epitope recognized is located between amino acids 504-617 of human HSP70, a region that has been shown to be involved in stress-induced nucleolar localization.

Target Information

HSP70 is a Heat shock protein (HSP) which are expressed in response to various biological stresses, including high temperatures. There are several major families of HSPs that include HSP70, there are HSP90 and HSP100. The HSP70 family is a set of highly conserved proteins that are induced by a variety of biological stresses, including heat stress, in every organism in which the proteins have been examined. The human HSP70 family members include: HSP70, a protein which is strongly inducible in all organisms but which is also constitutively expressed in primate cells; HSP72, a 72 kDa protein that is induced exclusively under stress conditions; HSC70, or cognate protein, is a 72 kDa, constitutively expressed, protein which is involved in the uncoating of clathrin coated vesicles; GRP78, or BiP, is a glucose regulated 78 kDa protein localized in the endoplasmic reticulum; and p75, or HSP75, a 75 kDa protein that is found within the mitochondria. Further, HSP70 is encoded by an intronless gene and, in conjunction with other heat shock proteins, HSP70 stabilizes existing proteins against aggregation and mediates the folding of newly translated proteins in the cytosol and organelles. HSP70 is also involved in the ubiquitin-proteasome pathway through interaction with the AU-rich element RNA-binding protein 1. The HSP70 gene is located in the major histocompatibility complex class III region, in a cluster with two closely related genes which encode similar proteins.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

gp130 Activates Mitochondrial Dynamics for Hepatocyte Survival in a Model of Steatohepatitis.

Biomedicines

Shunkina D,Dakhnevich A,Shunkin E,Khaziakhmatova O,Shupletsova V,Vulf M,Komar A,Kirienkova E,Litvinova L

MA3-006 was used in Gel Shift, Immunocytochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot to investigate the effect of gp130 on the components of mitochondrial dynamics in a cellular model of steatohepatitis.

Sun Jan 29 00:00:00 UTC 2023

Aspergillus fumigatus hijacks human p11 to redirect fungal-containing phagosomes to non-degradative pathway.

Cell host & microbe

Jia LJ,Rafiq M,Radosa L,Hortschansky P,Cunha C,Cseresnyés Z,Krüger T,Schmidt F,Heinekamp T,Straßburger M,Löffler B,Doenst T,Lacerda JF,Campos A,Figge MT,Carvalho A,Kniemeyer O,Brakhage AA

MA3-006 was used in Western Blot to reveal the role of p11 in mediating fungal phagosome evasion.

Wed Mar 08 00:00:00 UTC 2023

Local synthesis of the phosphatidylinositol-3,4-bisphosphate lipid drives focal adhesion turnover.

Developmental cell

Posor Y,Kampyli C,Bilanges B,Ganguli S,Koch PA,Wallroth A,Morelli D,Jenkins M,Alliouachene S,Deltcheva E,Baum B,Haucke V,Vanhaesebroeck B

MA3-006 was used in Gel Shift, Immunocytochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot to show that reduced growth factor signaling sensed by protein kinase N, an mTORC2 target and effector of RhoA, synergizes with the adhesion disassembly factor DEPDC1B to induce local synthesis of PtdIns(3,4)P2 by PI3K-C2β.

Mon Jul 25 00:00:00 UTC 2022

Terminal Uridylyltransferases TUT4/7 Regulate microRNA and mRNA Homeostasis.

Cells

Zhang P,Frederick MI,Heinemann IU

MA3-006 was used in Western Blotting to investigate the role of uridylation-dependent decay in maintaining the transcriptome by transcriptionally profiling TUT4/7 deleted cells.

Wed Nov 23 00:00:00 UTC 2022

Nanoscale segregation of channel and barrier claudins enables paracellular ion flux.

Nature communications

Gonschior H,Schmied C,Van der Veen RE,Eichhorst J,Himmerkus N,Piontek J,Günzel D,Bleich M,Furuse M,Haucke V,Lehmann M

MA3-006 was used in Sample Preparation to suggest that nanoscale segregation of distinct channel-forming claudins enables barrier function combined with specific paracellular ion flux across tight junctions.

Thu Aug 25 00:00:00 UTC 2022

Neuroprotective Effects of Asparagus officinalis Stem Extract in Transgenic Mice Overexpressing Amyloid Precursor Protein.

Journal of immunology research

Peng Z,Bedi S,Mann V,Sundaresan A,Homma K,Gaskey G,Kowada M,Umar S,Kulkarni AD,Eltzschig HK,Doursout MF

MA3-006 was used in Immunohistochemistry to study the neuroprotective effects of Asparagus officinalis stem extract in transgenic mice overexpressing amyloid precursor protein.

Mon Dec 06 00:00:00 UTC 2021

Novel Small Molecule Hsp90/Cdc37 Interface Inhibitors Indirectly Target K-Ras-Signaling.

Cancers

Siddiqui FA,Parkkola H,Vukic V,Oetken-Lindholm C,Jaiswal A,Kiriazis A,Pavic K,Aittokallio T,Salminen TA,Abankwa D

Published figure using HSP70 monoclonal antibody (Product # MA3-006) in Western Blot

Tue Feb 23 00:00:00 UTC 2021

Basic Limonoid modulates Chaperone-mediated Proteostasis and dissolve Tau fibrils.

Scientific reports

Gorantla NV,Das R,Chidambaram H,Dubey T,Mulani FA,Thulasiram HV,Chinnathambi S

MA3-006 was used in Western Blot to show that HSF1 in downstream increased the transcription of Hsp70 thus, aggravating cytosolic Hsp70 levels that can channel clearance of aberrant Tau.

Wed Mar 04 00:00:00 UTC 2020

Intersectin-Mediated Clearance of SNARE Complexes Is Required for Fast Neurotransmission.

Cell reports

Jäpel M,Gerth F,Sakaba T,Bacetic J,Yao L,Koo SJ,Maritzen T,Freund C,Haucke V

MA3-006 was used in Western Blotting to identify a key molecular mechanism that underlies exo-endocytic coupling during fast neurotransmitter release at central synapses.

Tue Jan 14 00:00:00 UTC 2020

Biomarker-based assessment of the muscle maintenance and energy status of anurans from an extremely seasonal semi-arid environment, the Brazilian Caatinga.

Comparative biochemistry and physiology. Part A, Molecular & integrative physiology

Madelaire CB,Gomes FR,Sokolova I

MA3-006 was used in Western Blot to investigate seasonal variation of metabolic biomarkers using muscle tissues from three species of anurans.

Sat Feb 01 00:00:00 UTC 2020

Visualizing tRNA-dependent mistranslation in human cells.

RNA biology

Lant JT,Berg MD,Sze DHW,Hoffman KS,Akinpelu IC,Turk MA,Heinemann IU,Duennwald ML,Brandl CJ,O'Donoghue P

MA3-006 was used in Western Blotting to study tRNA-dependent mistranslation in human cells.

Fri Nov 02 00:00:00 UTC 2018

Inhibition of heat shock protein (Hsp) 90 potentiates the antiproliferative and pro-apoptotic effects of 2-(4'fluoro-phenylamino)-4H-1,3-thiazine[6,5-b]indole in A2780cis cells.

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Solárová Z,Kello M,Varinská L,Budovská M,Solár P

MA3006 was used in western blot to elucidate the antiproliferative and pro-apoptotic effects of thiazine[6,5-b]indole

Sun Jan 01 00:00:00 UTC 2017

Short-term molecular and physiological responses to heat stress in neritic copepods Acartia tonsa and Eurytemora affinis.

Comparative biochemistry and physiology. Part A, Molecular & integrative physiology

Rahlff J,Peters J,Moyano M,Pless O,Claussen C,Peck MA

MA3006 was used in western blot to measure biochemical and organismal responses to acute heat stress on two neritic copepods

Sun Jan 01 00:00:00 UTC 2017

Loss of thermal refugia near equatorial range limits.

Global change biology

Lima FP,Gomes F,Seabra R,Wethey DS,Seabra MI,Cruz T,Santos AM,Hilbish TJ

MA3-006 was used in western blot to examine the effect of sun exposure on of thermal refugia

Fri Jan 01 00:00:00 UTC 2016

Non-lethal heat shock increases tolerance to metal exposure in brine shrimp.

Environmental research

Pestana JLT,Novais SC,Norouzitallab P,Vandegehuchte MB,Bossier P,De Schamphelaere KAC

MA3006 was used in western blot to test if exposure to non-lethal heat shock increases the tolerance of Artemia franciscana to subsequent metal exposure

Tue Nov 01 00:00:00 UTC 2016

Chronic hyperosmotic stress interferes with immune homeostasis in striped catfish (Pangasianodon hypophthalmus, S.) and leads to excessive inflammatory response during bacterial infection.

Fish & shellfish immunology

Schmitz M,Douxfils J,Mandiki SN,Morana C,Baekelandt S,Kestemont P

MA3006 was used in western blot to assess the effect of salinity on catfish's susceptibility to bacterial infection

Mon Aug 01 00:00:00 UTC 2016

Responses to thermal and salinity stress in wild and farmed Pacific oysters Crassostrea gigas.

Comparative biochemistry and physiology. Part A, Molecular & integrative physiology

Yang CY,Sierp MT,Abbott CA,Li Y,Qin JG

MA3-006 was used in western blot to study salinity and thermal stress in wild and farmed Pacific oysters Crassostrea gigas

Tue Nov 01 00:00:00 UTC 2016

Extended Synaptotagmin (ESyt) Triple Knock-Out Mice Are Viable and Fertile without Obvious Endoplasmic Reticulum Dysfunction.

PloS one

Sclip A,Bacaj T,Giam LR,Südhof TC

MA3006 was used in western blot to investigate the function of extended synaptotagmins in knock out mice

Mon Jul 24 00:00:00 UTC 2017

Histological, ultrastructural and heat shock protein 70 (HSP70) responses to heat stress in the sea cucumber Apostichopus japonicus.

Fish & shellfish immunology

Xu D,Sun L,Liu S,Zhang L,Yang H

MA3-006 was used in immunohistochemistry and western blot to study HSP70 in Apostichopus japonicus

Sat Aug 01 00:00:00 UTC 2015

Contrasting roles for MyoD in organizing myogenic promoter structures during embryonic skeletal muscle development.

Developmental dynamics : an official publication of the American Association of Anatomists

Cho OH,Mallappa C,Hernández-Hernández JM,Rivera-Pérez JA,Imbalzano AN

MA3-006 was used in western blot to study the temporal regulation of MyoD in skeletal muscle differentiation

Thu Jan 01 00:00:00 UTC 2015

Loss of Gadkin Affects Dendritic Cell Migration In Vitro.

PloS one

Schachtner H,Weimershaus M,Stache V,Plewa N,Legler DF,Höpken UE,Maritzen T

MA3-006 was used in western blot to determine the affects of dendritic cell migration in vitro by loss of gadkin

Mon Jun 20 00:00:00 UTC 2016

Non-Lethal Heat Shock of the Asian Green Mussel, Perna viridis, Promotes Hsp70 Synthesis, Induces Thermotolerance and Protects Against Vibrio Infection.

PloS one

Aleng NA,Sung YY,MacRae TH,Abd Wahid ME

MA3-006 was used in western blot to test if non-lethal heat shock of Perna viridis promotes the production of Hsp70 and enhances its resistance to stresses

Wed May 18 00:00:00 UTC 2016

Non-Lethal Heat Shock of the Asian Green Mussel, Perna viridis, Promotes Hsp70 Synthesis, Induces Thermotolerance and Protects Against Vibrio Infection.

PloS one

Aleng NA,Sung YY,MacRae TH,Abd Wahid ME

MA3-006 was used in western blot to test if non-lethal heat shock of Perna viridis promotes the production of Hsp70 and enhances its resistance to stresses

Wed May 18 00:00:00 UTC 2016

Environmental heat stress induces epigenetic transgenerational inheritance of robustness in parthenogenetic Artemia model.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Norouzitallab P,Baruah K,Vandegehuchte M,Van Stappen G,Catania F,Vanden Bussche J,Vanhaecke L,Sorgeloos P,Bossier P

MA3-006 was used in western blot to study epigenetic transgenerational inheritance of heat stress tolerance in parthenogenetic Artemia

Fri Aug 01 00:00:00 UTC 2014

Thermal tolerance in juvenile King George whiting (Sillaginodes punctata) reduces as fish age and this reduction coincides with migration to deeper colder water.

Comparative biochemistry and physiology. Part A, Molecular & integrative physiology

Meakin CA,Qin JG,Pogson LD,Abbott CA

MA3-006 was used in immunocytochemistry and western blot to study heat schock protein expression and thermal tolerance in juvenile and adult King George whiting

Sun Jun 01 00:00:00 UTC 2014

Non-lethal heat shock increased Hsp70 and immune protein transcripts but not Vibrio tolerance in the white-leg shrimp.

PloS one

Loc NH,Macrae TH,Musa N,Bin Abdullah MD,Abdul Wahid ME,Sung YY

MA3-006 was used in western blot to study the lack of effect of abrupt heat shock on the susceptibility of white-leg shrimp to Vibrio infection despite increased expression of Hsp70 and immune-related proteins

Sun Jun 15 00:00:00 UTC 2014

Non-lethal heat shock increased Hsp70 and immune protein transcripts but not Vibrio tolerance in the white-leg shrimp.

PloS one

Loc NH,Macrae TH,Musa N,Bin Abdullah MD,Abdul Wahid ME,Sung YY

Sun Jun 15 00:00:00 UTC 2014

UV-B exposure causes DNA damage and changes in protein expression in northern pike (Esox lucius) posthatched embryos.

Photochemistry and photobiology

Vehniäinen ER,Vähäkangas K,Oikari A

Published figure using HSP70 monoclonal antibody (Product # MA3-006) in Immunohistochemistry

Mon Jun 18 00:00:00 UTC 2012

The oncogenic TBC domain protein USP6/TRE17 regulates cell migration and cytokinesis.

Biology of the cell

Rueckert C,Haucke V

MA3-006 was used in western blot to investigate the important role of USP6 in regulation of cell migration and division

Sun Jan 01 00:00:00 UTC 2012

Enhancement of Hsp70 synthesis protects common carp, Cyprinus carpio L., against lethal ammonia toxicity.

Journal of fish diseases

Sung YY,Roberts RJ,Bossier P

MA3-006 was used in western blot to study the ability of a prickly pear cactus extract to increase Hsp70 levels and protect common carp against ammonia toxicity

Wed Aug 01 00:00:00 UTC 2012

Priming the prophenoloxidase system of Artemia franciscana by heat shock proteins protects against Vibrio campbellii challenge.

Fish & shellfish immunology

Baruah K,Ranjan J,Sorgeloos P,Macrae TH,Bossier P

MA3-006 was used in western blot to detect immune system priming by heat shock protein 70

Fri Jul 01 00:00:00 UTC 2011

Does domestication process affect stress response in juvenile Eurasian perch Perca fluviatilis?

Comparative biochemistry and physiology. Part A, Molecular & integrative physiology

Douxfils J,Mandiki SN,Marotte G,Wang N,Silvestre F,Milla S,Henrotte E,Vandecan M,Rougeot C,Mélard C,Kestemont P

MA3-006 was used in western blot to study the effect of domestication on the stress response in juvenile Eurasian perch Perca fluviatilis

Sun May 01 00:00:00 UTC 2011

Level of heat shock proteins decreases in individuals carrying B-chromosomes in the grasshopper Eyprepocnemis plorans.

Cytogenetic and genome research

Teruel M,Sørensen JG,Loeschcke V,Cabrero J,Perfectti F,Camacho JP

MA3-006 was used in western blot to investigate the effect of B-chromosome on heat shock protein expression in Eyprepocnemis plorans

Mon Nov 22 00:00:00 UTC 2010

Different rotavirus strains enter MA104 cells through different endocytic pathways: the role of clathrin-mediated endocytosis.

Journal of virology

Gutiérrez M,Isa P,Sánchez-San Martin C,Pérez-Vargas J,Espinosa R,Arias CF,López S

MA3-006 was used in blocking/activating experiment to investigate the mechanism for the rotavirus entry into host cells

Wed Sep 01 00:00:00 UTC 2010

Efficacy of heterologous and homologous heat shock protein 70s as protective agents to Artemia franciscana challenged with Vibrio campbellii.

Fish & shellfish immunology

Baruah K,Ranjan J,Sorgeloos P,Bossier P

MA3-006 was used in western blot to investigate the effect of different Hsp70 on protection against Vibrio campbellii

Mon Nov 01 00:00:00 UTC 2010

NF-κB is not directly responsible for photoresistance induced by fractionated light delivery in HT-29 colon adenocarcinoma cells.

Photochemistry and photobiology

Kuliková L,Mikeš J,Hýžďalová M,Palumbo G,Fedoročko P

MA3-006 was used in western blot to investigate molecular mechanisms of the photoresistance on HT-29 cells

Mon Mar 21 00:00:00 UTC 2011

Do historical sediments of pulp and paper industry contribute to the exposure of fish caged in receiving waters?

Journal of environmental monitoring : JEM

Oikari A,Lahti M,Meriläinen P,Afanasyev S,Krasnov A

MA3-006 was used in western blot to investigate the environmental influence of paper mills for fish health

Sat May 01 00:00:00 UTC 2010

Oxidative stress, protein carbonylation and heat shock proteins in the black tiger shrimp, Penaeus monodon, following exposure to endosulfan and deltamethrin.

Environmental toxicology and pharmacology

Dorts J,Silvestre F,Tu HT,Tyberghein AE,Phuong NT,Kestemont P

MA3-006 was used in western blot to study the cellular changes in the black tiger shrimp Penaeus monodon in response to

Tue Sep 01 00:00:00 UTC 2009

The role of p53 in the efficiency of photodynamic therapy with hypericin and subsequent long-term survival of colon cancer cells.

Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology

Mikes J,Koval' J,Jendzelovský R,Sacková V,Uhrinová I,Kello M,Kuliková L,Fedorocko P

MA3-006 was used in western blot to study the effect of p53 expression on the efficacy of photodynamic therapy and colon cancer recurrence

Sun Nov 01 00:00:00 UTC 2009

Surface expression of a C-terminal alpha-helix region in heat shock protein 72 on murine LL/2 lung carcinoma can be recognized by innate immune sentinels.

Molecular immunology

Tani F,Ohno M,Furukawa Y,Sakamoto M,Masuda S,Kitabatake N

MA3-006 was used in western blot to study the induction of heat shock protein 72 expression in murine LL/2 lung carcinoma

Wed Apr 01 00:00:00 UTC 2009

Sex-, gametogenesis, and tidal height-related differences in levels of HSP70 and metallothioneins in the Pacific oyster Crassostrea gigas.

Comparative biochemistry and physiology. Part A, Molecular & integrative physiology

Meistertzheim AL,Lejart M,Le Goïc N,Thébault MT

MA3-006 was used in western blot to study the effect of gene, gametogenesis, and tidal height on heat shock protein 70 and metallothionein expression in the Pacific oyster Crassostrea gigas

Sun Feb 01 00:00:00 UTC 2009

Bioinformatics and protein expression analyses implicate LEA proteins in the drought response of Collembola.

Journal of insect physiology

Bahrndorff S,Tunnacliffe A,Wise MJ,McGee B,Holmstrup M,Loeschcke V

MA3-006 was used in western blot to investigate the role of late embryogenesis abundant proteins in the drought response of Collembola

Sun Mar 01 00:00:00 UTC 2009

Dimeric heat shock protein 40 binds radial spokes for generating coupled power strokes and recovery strokes of 9 + 2 flagella.

The Journal of cell biology

Yang C,Owen HA,Yang P

MA3-006 was used in western blot to study the role of dimeric heat shock protein 40 in flagella function.

Mon Jan 28 00:00:00 UTC 2008

The effects of treated effluents on the intensity of papillomatosis and HSP70 expression in roach.

Ecotoxicology and environmental safety

Korkea-aho TL,Vehniäinen ER,Kukkonen JV,Taskinen J

MA3-006 was used in western blot to investigate the influence of treated pulp mill and municipal effluents on papillomatosis and heat shock protein 70 expression in Rutilus rutilus

Tue Jul 01 00:00:00 UTC 2008

Saccharomyces cerevisiae strains from traditional fermentations of Brazilian cachaça: trehalose metabolism, heat and ethanol resistance.

Antonie van Leeuwenhoek

Vianna CR,Silva CL,Neves MJ,Rosa CA

MA3-006 was used in western blot to investigate the trehalose metabolism of cachaca S. cerevisiae strains under stress conditions

Wed Jul 02 00:00:00 UTC 2008

Non-lethal heat shock protects gnotobiotic Artemia franciscana larvae against virulent Vibrios.

Fish & shellfish immunology

Yik Sung Y,Van Damme EJ,Sorgeloos P,Bossier P

MA3-006 was used in western blot to study the role of non-lethal heat shock in protecting gnotobiotic Artemia franciscana larvae against virulent Vibrios

Sun Apr 01 00:00:00 UTC 2007

Synergistic impacts of heat shock and spawning on the physiology and immune health of Crassostrea gigas: an explanation for summer mortality in Pacific oysters.

American journal of physiology. Regulatory, integrative and comparative physiology

Li Y,Qin JG,Abbott CA,Li X,Benkendorff K

MA3-006 was used in western blot to study how spawning affects thermotolerance in the Pacific oyster in summer with extremely high temperature

Sat Dec 01 00:00:00 UTC 2007

Is HSP70 involved in acclimation to cadmium in the Chinese crab, Eriocheir sinensis ?

Bulletin of environmental contamination and toxicology

Silvestre F,Trausch G,Devos P

MA3-006 was used in western blot to investigate the influence of heat shock protein 70 on Chinese crab acclimation to cadmium

Fri Jun 01 00:00:00 UTC 2007

Heat shock protein (hsp70) expression and thermal tolerance in sublethally heat-shocked eastern oysters Crassostrea virginica infected with the parasite Perkinsus marinus.

Diseases of aquatic organisms

Encomio VG,Chu FL

MA3-006 was used in western blot to investigate the protective effect of sublethal heat shock on Crassostrea virginica with Perkinsus marinus infection against lethal heat stress

Mon Jul 16 00:00:00 UTC 2007

Multiscale approach of fish responses to different types of environmental contaminations: a case study.

The Science of the total environment

Mayon N,Bertrand A,Leroy D,Malbrouck C,Mandiki SN,Silvestre F,Goffart A,Thomé JP,Kestemont P

MA3-006 was used in western blot to investigate the fish responses to environmental contaminations

Thu Aug 31 00:00:00 UTC 2006

Thermoprotection of synaptic transmission in a Drosophila heat shock factor mutant is accompanied by increased expression of Hsp83 and DnaJ-1.

Physiological genomics

Neal SJ,Karunanithi S,Best A,So AK,Tanguay RM,Atwood HL,Westwood JT

MA3-006 was used in western blot to study the thermoprotective mechanism for a Drosophila heat shock factor mutant

Tue May 16 00:00:00 UTC 2006

Temperature regulates hypoxia-inducible factor-1 (HIF-1) in a poikilothermic vertebrate, crucian carp (Carassius carassius).

The Journal of experimental biology

Rissanen E,Tranberg HK,Sollid J,Nilsson GE,Nikinmaa M

MA3-006 was used in western blot to study the role of temperature in regulating hypoxia-inducible factor-1 expression in a poikilothermic fish

Wed Mar 01 00:00:00 UTC 2006

Stress protein response in two sibling species of Marenzelleria (Polychaeta: Spionidae): is there an influence of acclimation salinity?

Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology

Blank M,Bastrop R,Jürss K

MA3-006 was used in western blot to study the effect of acclimation salinity on the stress protein response in two sibling species of Marenzelleria

Tue Aug 01 00:00:00 UTC 2006

Dimeric novel HSP40 is incorporated into the radial spoke complex during the assembly process in flagella.

Molecular biology of the cell

Yang C,Compton MM,Yang P

MA3-006 was used in western blot to study the incorporation of a novel HSP40 into the radial spoke assembly during flagella assembly

Tue Feb 01 00:00:00 UTC 2005

ATP production in Chlamydomonas reinhardtii flagella by glycolytic enzymes.

Molecular biology of the cell

Mitchell BF,Pedersen LB,Feely M,Rosenbaum JL,Mitchell DR

MA3-006 was used in western blot to study the role of in situ ATP synthesis in normal flagellar motility.

Sat Oct 01 00:00:00 UTC 2005

Characterization of a molecular chaperone present in the eukaryotic flagellum.

Eukaryotic cell

Shapiro J,Ingram J,Johnson KA

MA3-006 was used in western blot to study the role of the Hsp70 in the assembly and maintenance of the flagellum.

Thu Sep 01 00:00:00 UTC 2005

Aggresome formation.

Methods in molecular biology (Clifton, N.J.)

Corboy MJ,Thomas PJ,Wigley WC

MA3-006 was used in Gel Shift, Immunocytochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot to conclude that the centrosome may play a central role in protein quality control, sitting at the crossroads of protein folding, degradation, and aggregation.

Thu Jun 16 00:00:00 UTC 2005

A field methodology to study effects of UV radiation on fish larvae.

Water research

Häkkinen J,Oikari A

MA3-006 was used in western blot to investigate the effects of UV radiation on fish larvae

Thu Jul 01 00:00:00 UTC 2004

High sensitivity of northern pike larvae to UV-B but no UV-photoinduced toxicity of retene.

Aquatic toxicology (Amsterdam, Netherlands)

Häkkinen J,Vehniäinen E,Oikari A

MA3-006 was used in western blot to investigate the influence of UV-B on northern pike larvae

Wed Mar 10 00:00:00 UTC 2004

Rapid glucocorticoid receptor exchange at a promoter is coupled to transcription and regulated by chaperones and proteasomes.

Molecular and cellular biology

Stavreva DA,Müller WG,Hager GL,Smith CL,McNally JG

MA3-006 was used in immunocytochemistry to investigate the role of chaperones and proteasomes during glucocorticoid receptor exchange.

Thu Apr 01 00:00:00 UTC 2004

Peptidoglycan recognition protein tag7 forms a cytotoxic complex with heat shock protein 70 in solution and in lymphocytes.

The Journal of biological chemistry

Sashchenko LP,Dukhanina EA,Yashin DV,Shatalov YV,Romanova EA,Korobko EV,Demin AV,Lukyanova TI,Kabanova OD,Khaidukov SV,Kiselev SL,Gabibov AG,Gnuchev NV,Georgiev GP

MA3-006 was used in western blot to study the interaction between the peptidoglycan recognition protein tag7 and Hsp70 in solution and in lymphocytes

Fri Jan 16 00:00:00 UTC 2004

Targeted disruption of the heat shock transcription factor (hsf)-2 gene results in increased embryonic lethality, neuronal defects, and reduced spermatogenesis.

Genesis (New York, N.Y. : 2000)

Wang G,Zhang J,Moskophidis D,Mivechi NF

MA3-006 was used in western blot to study the effects of heat shock transcription factor-2 knockout

Thu May 01 00:00:00 UTC 2003

Photoinduced lethal and sublethal toxicity of retene, a polycyclic aromatic hydrocarbon derived from resin acid, to coregonid larvae.

Environmental toxicology and chemistry

Vehniäinen ER,Häkkinen J,Oikari A

MA3-006 was used in Western Blot to investigate retene toxicity in pelagial larvae.

Mon Dec 01 00:00:00 UTC 2003

Heat-shock protein (HSP70) response in the eastern oyster, Crassostrea virginica, exposed to PAHs sorbed to suspended artificial clay particles and to suspended field contaminated sediments.

Aquatic toxicology (Amsterdam, Netherlands)

Cruz-Rodríguez LA,Chu FL

MA3-006 was used in western blot to investigate the heat shock responses in the eastern oyster Crassostrea virginica

Wed Oct 30 00:00:00 UTC 2002

CFTR degradation and aggregation.

Methods in molecular medicine

Corboy MJ,Thomas PJ,Wigley WC

MA3-006 was used in Gel Shift, Immunocytochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot to discuss CFTR degradation and aggregation.

Thu Sep 05 00:00:00 UTC 2002

Reconstitution of a functional core polycomb repressive complex.

Molecular cell

Francis NJ,Saurin AJ,Shao Z,Kingston RE

MA3-006 was used in Western Blotting to define a functional core of PRC1 by reconstituting a stable complex using four recombinant PcG proteins.

Sat Sep 01 00:00:00 UTC 2001

Co-chaperones Bag-1, Hop and Hsp40 regulate Hsc70 and Hsp90 interactions with wild-type or mutant p53.

The EMBO journal

King FW,Wawrzynow A,Höhfeld J,Zylicz M

MA3-006 was used in immunoprecipitation to investigate the role of co-chaperones Bag-1, Hop and heat Shock Protein 40 in Hsc70 and heat Shock Protein 90 interactions with p53

Thu Nov 15 00:00:00 UTC 2001

Contribution of heat shock proteins to cell protection from complement-mediated lysis.

International immunology

Fishelson Z,Hochman I,Greene LE,Eisenberg E

MA3-006 was used in flow cytometry to investigate the protective roles of heat shock proteins during complement-mediated lysis

Wed Aug 01 00:00:00 UTC 2001

Insights into regulation and function of the major stress-induced hsp70 molecular chaperone in vivo: analysis of mice with targeted gene disruption of the hsp70.1 or hsp70.3 gene.

Molecular and cellular biology

Huang L,Mivechi NF,Moskophidis D

MA3-006 was used in Western Blotting to study the physiological role of the hsp70.1 and hsp70.3 proteins in vivo.

Sat Dec 01 00:00:00 UTC 2001

Individual subunits of the eukaryotic cytosolic chaperonin mediate interactions with binding sites located on subdomains of beta-actin.

The Journal of biological chemistry

Hynes GM,Willison KR

MA3-006 was used in ELISA to determine the interaction of individual subunits of CCT with newly synthesized b-actin.

Fri Jun 23 00:00:00 UTC 2000

Multiple components of the HSP90 chaperone complex function in regulation of heat shock factor 1 In vivo.

Molecular and cellular biology

Bharadwaj S,Ali A,Ovsenek N

MA3-006 was used in western blot to study the complexing process of heat shock factor 1 with the HSP90 chaperone machinery.

Wed Dec 01 00:00:00 UTC 1999

Heat shock response and protein degradation: regulation of HSF2 by the ubiquitin-proteasome pathway.

Molecular and cellular biology

Mathew A,Mathur SK,Morimoto RI

MA3-006 was used in western blot to investigate the regulation of heat shock transcription factor 2 by ubiquitin-proteasome

Tue Sep 01 00:00:00 UTC 1998

Effect of different environmental variables on the synthesis of Hsp70 in Raphidocelis subcapitata.

Comparative biochemistry and physiology. Part A, Molecular & integrative physiology

Bierkens J,Van de Perre WV,Maes J

MA3-006 was used in ELISA to evaluate heat Shock Protein 70 in Raphidocelis subcapitata as an environmental monitor

Fri May 01 00:00:00 UTC 1998

Transport of a novel complex in the cytoplasmic matrix of Chlamydomonas flagella.

Proceedings of the National Academy of Sciences of the United States of America

Piperno G,Mead K

MA3-006 was used in western blot to study the mechanisms for the protein transport important for maintaining Chlamydomonas flagella

Tue Apr 29 00:00:00 UTC 1997

Synthesis and turnover of embryonic sea urchin ciliary proteins during selective inhibition of tubulin synthesis and assembly.

Molecular biology of the cell

Stephens RE

MA3-006 was used in western blot to study the role of tubulin synthesis and assembly in sea urchin ciliogenesis.

Sat Nov 01 00:00:00 UTC 1997

HSP70 binding sites in the tumor suppressor protein p53.

The Journal of biological chemistry

Fourie AM,Hupp TR,Lane DP,Sang BC,Barbosa MS,Sambrook JF,Gething MJ

MA3-006 was used in western blot to identify the binding sites of HSP70 in p53

Fri Aug 01 00:00:00 UTC 1997

Identification of a molecular chaperone in the eukaryotic flagellum and its localization to the site of microtubule assembly.

Journal of cell science

Bloch MA,Johnson KA

MA3-006 was used in western blot to identify a molecular chaperone in the eukaryotic flagellum and investigate its localization

Wed Nov 01 00:00:00 UTC 1995

Cloning and subcellular localization of human mitochondrial hsp70.

The Journal of biological chemistry

Bhattacharyya T,Karnezis AN,Murphy SP,Hoang T,Freeman BC,Phillips B,Morimoto RI

MA3-006 was used in western blot to characterize human mitochondrial hsp70 and investigate its cellular distribution

Fri Jan 27 00:00:00 UTC 1995

The RCC1 protein interacts with Ran, RanBP1, hsc70, and a 340-kDa protein in Xenopus extracts.

The Journal of biological chemistry

Saitoh H,Dasso M

MA3-006 was used in western blot to investigate the interactions of the RCC1 protein with Ran, RanBP1, hsc70, and a 340-kDa protein in Xenopus extracts

Fri May 05 00:00:00 UTC 1995

Interaction between heat shock factor and hsp70 is insufficient to suppress induction of DNA-binding activity in vivo.

Molecular and cellular biology

Rabindran SK,Wisniewski J,Li L,Li GC,Wu C

MA3-006 was used in western blot to investigate the association of heat shock factor with heat shock proteins and its effect on DNA-binding activity.

Sat Oct 01 00:00:00 UTC 1994

The stress response to loss of signal recognition particle function in Saccharomyces cerevisiae.

The Journal of biological chemistry

Arnold CE,Wittrup KD

MA3-006 was used in western blot to investigate the consequences of depletion of SRP54p in budding yeast

Fri Dec 02 00:00:00 UTC 1994

Regulation of the human hsp70 promoter by p53.

Science (New York, N.Y.)

Agoff SN,Hou J,Linzer DI,Wu B

MA3-006 was used in immunoprecipitation to investigate the effect of p53 on HSP70 transcription

Fri Jan 01 00:00:00 UTC 1993

A constitutive form of heat-shock protein 70 is located in the outer membranes of mitochondria from rat liver.

FEBS letters

Lithgow T,Ryan M,Anderson RL,Høj PB,Hoogenraad NJ

MA3-006 was used in western blot to study the role of heat-shock protein 70 in the outer membranes of mitochondria from rat liver

Mon Oct 18 00:00:00 UTC 1993

Pork quality and the expression of stress protein Hsp 70 in swine.

Journal of animal science

van Laack RL,Faustman C,Sebranek JG

MA3-006 was used in western blot to study the expression of Hsp 70 in porcine tissue and to correlate its expression with porcine stress susceptibility

Mon Nov 01 00:00:00 UTC 1993

T-complex polypeptide-1 is a subunit of a heteromeric particle in the eukaryotic cytosol.

Nature

Lewis VA,Hynes GM,Zheng D,Saibil H,Willison K

MA3-006 was used in western blot to characterize the cytosolic T-complex polypeptide 1

Thu Jul 16 00:00:00 UTC 1992

Bioinformatics

Protein Aliases: 68 kDa heat shock protein; 70 kilodalton heat shock protein; 87A locus; 87C locus; CG31359-PA; CG31366-PA; dnaK-type molecular chaperone HSP70-1; epididymis secretory protein Li 103; epididymis secretory sperm binding protein Li 5a; heat shock 10kDa protein 1-like; heat shock 70; Heat shock 70 kDa protein 1; Heat shock 70 kDa protein 1-Hom; Heat shock 70 kDa protein 1-like; heat shock 70 kDa protein 1/2; Heat shock 70 kDa protein 1A; heat shock 70 kDa protein 1A/1B; Heat shock 70 kDa protein 1B; Heat shock 70 kDa protein 1L; Heat shock 70 kDa protein 2; Heat shock 70 kDa protein 3; Heat shock 70 kDa protein 4; Heat shock 70 kDa-like protein 1; Heat shock 70-related protein APG-2; heat shock 70kD protein 1A; heat shock 70kD protein 1B; heat shock 70kD protein 2; heat shock 70kD protein 4; heat shock 70kD protein-like 1; heat shock 70kDa protein 1-like; heat shock 70kDa protein 1A; heat shock 70kDa protein 1B; heat shock 70kDa protein 2; heat shock 70kDa protein 4; Heat shock protein; heat shock protein 70; heat shock protein 70-1; Heat shock protein 70-1l; heat shock protein 70.1; Heat shock protein 70.2; Heat shock protein 70Aa; Heat shock protein 70Ab; Heat shock protein 70Ba; Heat shock protein 70Bb; Heat shock protein 70Bc; heat shock protein alpha 2; heat shock protein Apg-2; heat shock protein cognate 70, testis; Heat shock protein family A member 1A; Heat shock protein family A member 1B; Heat shock protein family A member 1L; Heat shock protein family A member 2; Heat shock protein family H member 2; heat shock protein, 110 kDa; heat shock protein, 70 kDa 1; heat shock protein, 70 kDa 2; heat shock protein, 70 kDa 3; heat shock protein-70; heat shock-induced protein; Heat shock-related 70 kDa protein 2; heat shock-related protein hsc70t; heat-shock protein 70; Heat-shock-protein-70Aa; Heat-shock-protein-70Bb; HEL S 103; HSP; Hsp68; HSP70 1; HSP70 1B; HSP70 2; hsp70 87A; HSP70 AceyltK77; hsp70 RY; HSP70-1; HSP70-1/HSP70-2; HSP70-2; HSP70-87A7; HSP70-87C1; HSP70-Hom; HSP70.1; HSP70.1/2; HSP70.1/HSP70.2; HSP70.2; HSP70.3; Hsp70Aa-PA; Hsp70Bb-PA; HSP70RY; HSPA2; HSPA4; Hspa70; HST; inducible heat shock protein 70; Ischemia responsive 94 kDa protein; Major heat shock 70 kDa protein Aa; Major heat shock 70 kDa protein Ab; Major heat shock 70 kDa protein Ba; Major heat shock 70 kDa protein Bb; Major heat shock 70 kDa protein Bc; protein 70; Spermatid-specific heat shock protein 70; Testis-specific heat shock protein-related; testis-specific heat shock protein-related gene hst70

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Gene Aliases: 70kDa; 87A; AI317151; APG-2; APG2; Bb; CG18743; CG31359; CG31366; CG31449; CG6489; dHsp70; dhsp70Aa; Dm-hsp70; Dmel\CG31359; Dmel\CG31366; Dmel_CG31359; Dmel_CG31366; DmHSP70AA; DMHSP7D1; Hcp70.1; Hcp70.2; HEL-S-103; HEL-S-5a; HS24/P52; hs70; Hsc70t; Hsp 70; Hsp-70; Hsp-70Aa; Hsp110; hsp68; HSP70; hsp70 87A; hsp70 87C; hsp70 Aa; hsp70(87A); Hsp70(87C); HSP70-1; HSP70-1A; HSP70-1B; HSP70-1L; HSP70-2; HSP70-3; HSP70-HOM; HSP70.1; HSP70.2; Hsp70.3; Hsp70A; Hsp70A1; HSP70A2; Hsp70A7d; Hsp70Aa; Hsp70Ab; Hsp70B; Hsp70Ba; Hsp70Bb; Hsp70Bbc; Hsp70Bc; HSP70I; Hsp70RY; HSP70T; HSP72; HSPA1; HSPA1A; HSPA1B; HSPA1L; HSPA2; HSPA4; HSPH2; Hspt70; Hst70; HSX70; hum70t; Irp94; mKIAA4025; Msh5; RY; SBAB-707F1.4; SBAB-711D2.2

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Function(s)

ATP binding virus receptor activity G-protein coupled receptor binding receptor binding protein binding ATPase activity enzyme binding heat shock protein binding ubiquitin protein ligase binding ATPase activity, coupled histone deacetylase binding protein binding involved in protein folding unfolded protein binding C3HC4-type RING finger domain binding cadherin binding involved in cell-cell adhesion glycolipid binding protease binding double-stranded RNA binding poly(A) RNA binding protein N-terminus binding NF-kappaB binding molecular_function nucleotide binding RNA polymerase II transcription factor binding protein complex binding activating transcription factor binding

Process(es)

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HSP70 Monoclonal Antibody (3A3)

HSP70 Antibody (MA3-006) in ICC/IF

Product Details

MA3-006

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

ELISA (ELISA)

Immunoprecipitation (IP)

Neutralization (Neu)

Gel Shift (GS)

Miscellaneous PubMed (MISC)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

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Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

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