Invitrogen

HSP70 Monoclonal Antibody (C92F3A-5), FITC

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Datasheet
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Datasheet
Protocols
Questions & Answers

Product Details

MA5-45091

Applications
Tested Dilution
Publications

Western Blot (WB)

1:1,000
-

Immunohistochemistry (PFA fixed) (IHC (PFA))

1:10,000
-

Immunocytochemistry (ICC/IF)

1:1,000
-

Flow Cytometry (Flow)

1:1,000
-

ELISA (ELISA)

Assay-dependent
-

Functional Assay (Functional)

Assay-dependent
-

Immunomicroscopy (IM)

Assay-dependent
-

Inhibition Assays (IA)

Assay-dependent
-

Antibody Microarray (AM)

Assay-dependent
-
Product Specifications

Species Reactivity

Bovine, Dog, C. elegans, Chicken, Fruit fly, Fish, Guinea pig, Hamster, Human, Mouse, Non-human primate, Sheep, Pig, Rabbit, Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

C92F3A-5

Immunogen

Human HSP70

Conjugate

FITC FITC FITC

Excitation/Emission Max

498/517 nm View spectra spectra

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

9.1mM sodium bicarbonate/PBS, pH 7.4, with 640.91mM DMSO, 136.36mM ethanolamine

Contains

no preservative

Storage conditions

4°C, store in dark

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2931545

Product Specific Information

1 µg/mL of MA5-45091 was sufficient for detection of HSP70 in 20 µg of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.|Detects approximately 70kDa. Does not cross-react with HSC70 (HSP73).

Target Information

HSP70 is a Heat shock protein (HSP) which are expressed in response to various biological stresses, including high temperatures. There are several major families of HSPs that include HSP70, there are HSP90 and HSP100. The HSP70 family is a set of highly conserved proteins that are induced by a variety of biological stresses, including heat stress, in every organism in which the proteins have been examined. The human HSP70 family members include: HSP70, a protein which is strongly inducible in all organisms but which is also constitutively expressed in primate cells; HSP72, a 72 kDa protein that is induced exclusively under stress conditions; HSC70, or cognate protein, is a 72 kDa, constitutively expressed, protein which is involved in the uncoating of clathrin coated vesicles; GRP78, or BiP, is a glucose regulated 78 kDa protein localized in the endoplasmic reticulum; and p75, or HSP75, a 75 kDa protein that is found within the mitochondria. Further, HSP70 is encoded by an intronless gene and, in conjunction with other heat shock proteins, HSP70 stabilizes existing proteins against aggregation and mediates the folding of newly translated proteins in the cytosol and organelles. HSP70 is also involved in the ubiquitin-proteasome pathway through interaction with the AU-rich element RNA-binding protein 1. The HSP70 gene is located in the major histocompatibility complex class III region, in a cluster with two closely related genes which encode similar proteins.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: 68 kDa heat shock protein; 70 kda heat shock protein-2; 70 kilodalton heat shock protein; 87A locus; 87C locus; CG31359-PA; CG31366-PA; chaperone; dnaK-type molecular chaperone HSP70-1; epididymis secretory protein Li 103; epididymis secretory sperm binding protein Li 5a; heat shock 10kDa protein 1-like; heat shock 70; heat shock 70 kD protein 2; heat shock 70 kD protein 3; Heat shock 70 kDa protein 1; Heat shock 70 kDa protein 1-Hom; Heat shock 70 kDa protein 1-like; heat shock 70 kDa protein 1/2; Heat shock 70 kDa protein 1A; Heat shock 70 kDa protein 1A/1B; Heat shock 70 kDa protein 1B; Heat shock 70 kDa protein 1L; Heat shock 70 kDa protein 2; Heat shock 70 kDa protein 3; Heat shock 70 kDa protein 4; Heat shock 70 kDa-like protein 1; Heat shock 70-related protein APG-2; heat shock 70kD protein 1A; heat shock 70kD protein 1B; heat shock 70kD protein 2; heat shock 70kD protein 4; heat shock 70kD protein-like 1; heat shock 70kDa protein 1-like; heat shock 70kDa protein 1A; heat shock 70kDa protein 1B; heat shock 70kDa protein 2; heat shock 70kDa protein 3; heat shock 70kDa protein 4; heat shock 70kDa protein 4-like; Heat shock protein; heat shock protein 70; heat shock protein 70-1; Heat shock protein 70-1l; heat shock protein 70.1; Heat shock protein 70.2; Heat shock protein 70Aa; Heat shock protein 70Ab; Heat shock protein 70Ba; Heat shock protein 70Bb; Heat shock protein 70Bc; heat shock protein alpha 2; heat shock protein Apg-2; heat shock protein cognate 70, testis; Heat shock protein family A member 1A; Heat shock protein family A member 1B; Heat shock protein family A member 1L; Heat shock protein family A member 2; Heat shock protein family H member 2; heat shock protein, 110 kDa; heat shock protein, 70 kDa 1; heat shock protein, 70 kDa 2; heat shock protein, 70 kDa 3; heat shock protein-70; heat shock-induced protein; Heat shock-related 70 kDa protein 2; heat shock-related protein hsc70t; heat-shock 70-kilodalton protein 1B; heat-shock protein 70; Heat-shock-protein-70Aa; Heat-shock-protein-70Bb; HEL S 103; HSP; Hsp68; HSP70 1; HSP70 1B; HSP70 2; hsp70 87A; HSP70 AceyltK77; hsp70 RY; HSP70-1; HSP70-1/HSP70-2; HSP70-2; HSP70-87A7; HSP70-87C1; HSP70-Hom; HSP70.1; HSP70.1/2; HSP70.1/HSP70.2; HSP70.2; HSP70.3; Hsp70Aa-PA; Hsp70Bb-PA; HSP70RY; HSPA2; HSPA4; Hspa70; HST; inducible heat shock protein 70; Ischemia responsive 94 kDa protein; Major heat shock 70 kDa protein Aa; Major heat shock 70 kDa protein Ab; Major heat shock 70 kDa protein Ba; Major heat shock 70 kDa protein Bb; Major heat shock 70 kDa protein Bc; protein 70; Spermatid-specific heat shock protein 70; Testis-specific heat shock protein-related; testis-specific heat shock protein-related gene hst70

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Gene Aliases: 70kDa; 87A; AI317151; APG-2; APG2; Bb; BOS_11010; BOS_21593; BOS_21641; BOS_8056; CG18743; CG31359; CG31366; CG31449; CG6489; dHsp70; dhsp70Aa; Dm-hsp70; Dmel\CG31359; Dmel\CG31366; Dmel_CG31359; Dmel_CG31366; DmHSP70AA; DMHSP7D1; Hcp70.1; Hcp70.2; HEL-S-103; HEL-S-5a; HS24/P52; hs70; Hsc70t; Hsp 70; Hsp-70; Hsp-70Aa; Hsp110; hsp68; HSP70; hsp70 87A; hsp70 87C; hsp70 Aa; hsp70(87A); Hsp70(87C); HSP70-1; HSP70-1A; HSP70-1B; HSP70-1L; HSP70-2; HSP70-3; HSP70-HOM; HSP70.1; HSP70.2; Hsp70.3; Hsp70A; Hsp70A1; HSP70A2; Hsp70A7d; Hsp70Aa; Hsp70Ab; Hsp70B; Hsp70Ba; Hsp70Bb; Hsp70Bbc; Hsp70Bc; HSP70I; Hsp70RY; HSP70T; HSP72; HSPA1; HSPA1A; HSPA1B; HSPA1L; HSPA2; HSPA3; HSPA4; HSPH2; Hspt70; Hst70; HSX70; hum70t; I79_004332; Irp94; mKIAA4025; Msh5; RY; SBAB-707F1.4; SBAB-711D2.2

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UniProt ID: (Dog) Q7YQC6, (Pig) Q6S4N2, (Human) P0DMV8, (Bovine) Q27975, (Human) P34931, (Bovine) P0CB32, (Pig) A5A8V7, (Human) P54652, (Bovine) P34933, (Dog) Q2TFN9, (Human) P34932, (Fruit fly) P02825, (Fruit fly) Q8INI8, (Mouse) Q61696, (Rat) P0DMW0, (Mouse) P17879, (Rat) P55063, (Mouse) P16627, (Rat) P14659, (Mouse) P17156, (Rat) O88600

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Entrez Gene ID: (Dog) 403612, (Pig) 396648, (Sheep) 100913152, (Human) 3303, (Bovine) 282254, (Human) 3304, (Human) 3305, (Bovine) 540190, (Sheep) 101117117, (Pig) 100144518, (Human) 3306, (Dog) 480355, (Bovine) 281827, (Sheep) 101110063, (Rabbit) 100352959, (Dog) 474680, (Sheep) 101122782, (Human) 3308, (Bovine) 536558, (Fruit fly) 48581, (Fruit fly) 48582, (Mouse) 193740, (Chinese hamster) 100760510, (Rat) 24472, (Rat) 294254, (Mouse) 15511, (Rat) 24963, (Mouse) 15482, (Rat) 60460, (Chinese hamster) 100764750, (Mouse) 15512, (Mouse) 15525, (Rat) 266759, (Chinese hamster) 100764628, (Rabbit) 100338049, (C. elegans) 172757

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Function(s)
ATP binding virus receptor activity G-protein coupled receptor binding receptor binding protein binding ATPase activity enzyme binding heat shock protein binding ubiquitin protein ligase binding ATPase activity, coupled histone deacetylase binding protein binding involved in protein folding unfolded protein binding C3HC4-type RING finger domain binding cadherin binding involved in cell-cell adhesion glycolipid binding protease binding double-stranded RNA binding poly(A) RNA binding protein N-terminus binding NF-kappaB binding molecular_function nucleotide binding RNA polymerase II transcription factor binding protein complex binding activating transcription factor binding misfolded protein binding
Process(es)
response to unfolded protein binding of sperm to zona pellucida protein refolding regulation of cellular response to heat positive regulation of protein targeting to mitochondrion biological_process telomere maintenance DNA repair protein folding response to heat positive regulation of gene expression regulation of cell death regulation of protein ubiquitination negative regulation of protein ubiquitination positive regulation of interleukin-8 production cellular response to oxidative stress cellular response to heat regulation of mRNA stability ATP metabolic process viral entry into host cell protein stabilization positive regulation of NF-kappaB transcription factor activity negative regulation of cell death cellular heat acclimation positive regulation of nucleotide-binding oligomerization domain containing 2 signaling pathway negative regulation of inclusion body assembly cell-cell adhesion negative regulation of mitochondrial outer membrane permeabilization involved in apoptotic signaling pathway negative regulation of endoplasmic reticulum stress-induced intrinsic apoptotic signaling pathway positive regulation of endoribonuclease activity positive regulation of tumor necrosis factor-mediated signaling pathway positive regulation of mRNA endonucleolytic cleavage involved in unfolded protein response negative regulation of extrinsic apoptotic signaling pathway in absence of ligand negative regulation of protein phosphorylation negative regulation of gene expression positive regulation of protein binding negative regulation of apoptotic process negative regulation of DNA binding protein import into mitochondrial outer membrane chaperone-mediated protein complex assembly response to hypoxia heat shock-mediated polytene chromosome puffing kidney development positive regulation of angiogenesis negative regulation of cysteine-type endopeptidase activity involved in apoptotic process positive regulation of protein phosphorylation male meiosis male meiosis I multicellular organism development spermatid development positive regulation of cyclin-dependent protein serine/threonine kinase activity involved in G2/M transition of mitotic cell cycle synaptonemal complex disassembly positive regulation of T cell mediated cytotoxicity mRNA catabolic process defense response negative regulation of cell proliferation negative regulation of cell growth negative regulation of myeloid cell apoptotic process positive regulation of erythrocyte differentiation negative regulation of vasoconstriction response to stress spermatogenesis response to cold positive regulation of calcium-transporting ATPase activity determination of adult lifespan vesicle-mediated transport endoplasmic reticulum unfolded protein response cellular response to unfolded protein chaperone mediated protein folding requiring cofactor response to ischemia response to radiation response to mechanical stimulus neuron differentiation response to ethanol positive regulation of neuron differentiation cell differentiation
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