Invitrogen

HSP70/HSC70 Monoclonal Antibody (N27F3-4), APC

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Questions & Answers

Product Details

MA5-45093

Applications
Tested Dilution
Publications

Western Blot (WB)

1:1,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:100
-

Immunocytochemistry (ICC/IF)

1:50
-

Flow Cytometry (Flow)

Assay-dependent
-

Immunoprecipitation (IP)

Assay-dependent
-

Immunomicroscopy (IM)

Assay-dependent
-
Product Specifications

Species Reactivity

Bovine, Dog, C. elegans, Chicken, Fruit fly, Fish, Guinea pig, Hamster, Human, Mammal, Mouse, Non-human primate, Sheep, Plant, Pig, Rabbit, Rat, Xenopus

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

N27F3-4

Immunogen

Recombinant HSP70/HSC70

Conjugate

APC APC APC

Excitation/Emission Max

651/660 nm View spectra spectra

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

95.64mM phosphate/2.48mM MES, pH 7.2, with 0.5M EDTA

Contains

no preservative

Storage conditions

4°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2931547

Product Specific Information

1 µg/mL of MA5-45093 was sufficient for detection of HSP70/HSC70 in 20 µg of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.|Detects approximately 72 (HSP) and approximately 73kDa (HSC).

Target Information

HSPA4 (Heat Shock Protein Family A (Hsp70) Member 4) is a Protein Coding gene. Diseases associated with HSPA4 include Chagas Disease and Babesiosis. Among its related pathways are Regulation of activated PAK-2p34 by proteasome mediated degradation and Cellular responses to stimuli. An important paralog of this gene is HSPH1. HSPA8 (Heat Shock Protein Family A (Hsp70) Member 8) is a Protein Coding gene. Diseases associated with HSPA8 include Auditory System Disease and Borna Disease. Among its related pathways are Processing of Capped Intron-Containing Pre-mRNA and Selective autophagy. Gene Ontology (GO) annotations related to this gene include RNA binding and ubiquitin protein ligase binding. An important paralog of this gene is HSPA2.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: constitutive heat shock protein 70; epididymis luminal protein 33; epididymis secretory sperm binding protein Li 5a; epididymis secretory sperm binding protein Li 72p; Heat shock 70 kDa protein 1; Heat shock 70 kDa protein 4; Heat shock 70 kDa protein 8; Heat shock 70-related protein APG-2; heat shock 70kd protein 10; heat shock 70kd protein 10 (HSC71); heat shock 70kD protein 4; heat shock 70kD protein 8; heat shock 70kDa protein 1-like; heat shock 70kDa protein 4; heat shock 70kDa protein 4-like; heat shock 70kDa protein 8; heat shock 70kDa protein 8-like; heat shock cognate 70; heat shock cognate 71 kD protein; Heat shock cognate 71 kDa protein; heat shock cognate hsc73; heat shock cognate protein 54; Heat shock cognate protein 70; heat shock protein 8; heat shock protein A8; heat shock protein Apg-2; heat shock protein cognate 70; Heat shock protein family A member 8; Heat shock protein family H member 2; heat shock protein, 110 kDa; Hsc70 ATPase; hsp70 RY; HSP70RY; HSPA4/HSPA8; Ischemia responsive 94 kDa protein; LAP-1; Lipopolysaccharide-associated protein 1; LPS-associated protein 1; N-myristoyltransferase inhibitor protein 71

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Gene Aliases: 2410008N15Rik; 70kDa; AI317151; APG-2; APG2; BOS_14437; BOS_8056; HEL-33; HEL-S-5a; HEL-S-72p; HS24/P52; HSC54; HSC70; hsc70.I; HSC71; Hsc73; Hsp110; HSP70; Hsp70RY; HSP71; HSP73; HSPA1; HSPA10; hspa1l; HSPA4; HSPA8; HSPH2; I79_004332; I79_022974; Irp94; LAP-1; LAP1; mKIAA4025; NIP71; RY; XELAEV_18037286mg

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UniProt ID: (Dog) Q7YQC6, (Dog) Q2TFN9, (Human) P34932, (Bovine) P19120, (Human) P11142, (Chicken) O73885, (Rat) O88600, (Chinese hamster) P19378, (Rat) P63018, (Mouse) P63017

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Entrez Gene ID: (Dog) 403612, (Bovine) 536558, (Dog) 474680, (Sheep) 101122782, (Human) 3308, (Bovine) 281831, (Dog) 479406, (Sheep) 101112844, (Human) 3312, (Chicken) 395853, (Pig) 100511890, (Chinese hamster) 100764628, (Mouse) 15525, (Rat) 266759, (Chinese hamster) 100689472, (Rat) 24468, (Mouse) 15481, (Rabbit) 100338049, (Rabbit) 100348873, (Xenopus) 379760

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Function(s)
ATP binding protein binding ATPase activity, coupled unfolded protein binding G-protein coupled receptor binding phosphatidylserine binding ATPase activity enzyme binding MHC class II protein complex binding heat shock protein binding ubiquitin protein ligase binding poly(A) RNA binding C3HC4-type RING finger domain binding cadherin binding involved in cell-cell adhesion nucleotide binding RNA polymerase II transcription factor binding protein complex binding activating transcription factor binding receptor binding ADP binding
Process(es)
transcription, DNA-templated mRNA processing response to stress RNA splicing negative regulation of transcription, DNA-templated chaperone mediated protein folding requiring cofactor regulation of cell cycle negative regulation of protein phosphorylation response to heat positive regulation of gene expression negative regulation of gene expression positive regulation of protein binding negative regulation of apoptotic process negative regulation of DNA binding protein import into mitochondrial outer membrane chaperone-mediated protein complex assembly negative regulation of cell death kidney development positive regulation of angiogenesis protein folding protein refolding positive regulation of mRNA splicing, via spliceosome chaperone-mediated protein folding clathrin coat disassembly negative regulation of fibril organization mRNA splicing, via spliceosome protein targeting to lysosome response to unfolded protein neurotransmitter secretion cellular response to starvation viral process regulation of protein stability regulation of protein complex assembly regulation of mRNA stability ATP metabolic process regulation of protein complex stability chaperone-mediated autophagy late endosomal microautophagy protein targeting to lysosome involved in chaperone-mediated autophagy chaperone-mediated protein transport involved in chaperone-mediated autophagy cell-cell adhesion regulation of cellular response to heat regulation of protein import chaperone-mediated autophagy translocation complex disassembly regulation of transcription, DNA-templated post-Golgi vesicle-mediated transport membrane organization
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