Invitrogen

HSP90 alpha Monoclonal Antibody (3B6)

HSP90 alpha Antibody in Immunocytochemistry (ICC/IF)

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HSP90 alpha Antibody (MA3-010) in ICC/IF

Immunofluorescence analysis of HSP90 alpha was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with HSP90 alpha Monoclonal Antibody(3B6) (Product # MA3-010) at 1:100 dilution in 0.1% BSA and incubated overnight ... View More

HSP90 alpha Antibody in Immunohistochemistry (Paraffin) (IHC (P))

HSP90 alpha Antibody in Western Blot (WB)

HSP90 alpha Antibody in Immunohistochemistry (Frozen) (IHC (F))

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Product Details

MA3-010

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500

View 5 publications 5 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:20

Immunohistochemistry (Frozen) (IHC (F))

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:100

Product Specifications

Species Reactivity

Bovine, Human, Mouse, Rat

Published species

Bovine, Human, Mouse, Non-human primate, Rabbit

Host/Isotype

Mouse / IgG2b

Class

Monoclonal

Type

Antibody

Clone

3B6

Immunogen

Purified mouse HSP90.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

ascites, PBS

Contains

0.05% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_325458

Product Specific Information

MA3-010 detects heat shock protein 90 kDa (HSP90) from human, mouse, rat, and bovine tissues. This antibody does not detect chicken HSP90.

MA3-010 has been successfully used in Western blot, immunofluorescence, and immunohistochemistry (paraffin) procedures. By Western blot, this antibody detects a 90 kDa protein representing HSP90 from MCF7 cell extract. MA3-011 is more sensitive in Western blot procedures and should be used when maximum sensitivity is desired. MA3-010 does not immunoprecipitate HSP90 in any form (free or complexed).

The MA3-010 antigen is purified mouse HSP90.

Target Information

Heat shock proteins (HSP) are proteins that are expressed in response to various biological stress, including heat. HSP90 is a 90 kDa protein that is induced under stress conditions, but is also one of the most abundant cellular proteins found under non-stress conditions. HSP90 has been found to be associated with a number of other intracellular proteins, including steroid receptors, actin, tubulin, and some kinases. It has been suggested that HSP90 is necessary to maintain structural integrity of at least some steroid receptors to allow for ligand binding during receptor activation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Heat Shock Protein 90 Involvement in the Development of Idiopathic Epiretinal Membranes.

Investigative ophthalmology & visual science

Tosi GM,Regoli M,Altera A,Galvagni F,Arcuri C,Bacci T,Elia I,Realini G,Orlandini M,Bertelli E

MA3-010 was used in Immunohistochemistry-immunofluorescence to characterise cells of idiopathic epiretinal membranes (iERMs).

Wed Jul 01 00:00:00 UTC 2020

Heat Shock Protein 90 Involvement in the Development of Idiopathic Epiretinal Membranes.

Investigative ophthalmology & visual science

Tosi GM,Regoli M,Altera A,Galvagni F,Arcuri C,Bacci T,Elia I,Realini G,Orlandini M,Bertelli E

MA3-010 was used in Immunohistochemistry-immunofluorescence to characterise cells of idiopathic epiretinal membranes (iERMs).

Wed Jul 01 00:00:00 UTC 2020

The distinct agonistic properties of the phenylpyrazolosteroid cortivazol reveal interdomain communication within the glucocorticoid receptor.

Molecular endocrinology (Baltimore, Md.)

Yoshikawa N,Yamamoto K,Shimizu N,Yamada S,Morimoto C,Tanaka H

MA3-010 was used in western blot to study the mechanism for differential actions of various synthetic steroids on glucocorticoid receptor.

Sun May 01 00:00:00 UTC 2005

Oolemmal proteomics--identification of highly abundant heat shock proteins and molecular chaperones in the mature mouse egg and their localization on the plasma membrane.

Reproductive biology and endocrinology : RB&E

Calvert ME,Digilio LC,Herr JC,Coonrod SA

MA3-010 was used in immunohistochemistry to identify highly abundant heat shock proteins and chaperone proteins in mature mouse egg proteome.

Fri Feb 14 00:00:00 UTC 2003

Effect of stages of lactation on the concentration of a 90-kilodalton heat shock protein in bovine mammary tissue.

Journal of dairy science

Watanabe A,Miyamoto T,Katoh N,Takahashi Y

MA3-010 was used in western blot to investigate the differences of HSP90 expression in cow mammary tissue at various lactation stages

Wed Oct 01 00:00:00 UTC 1997

Agonist-free transformation of the glucocorticoid receptor in human B-lymphoma cells.

The Journal of steroid biochemistry and molecular biology

van den Berg JD,Smets LA,van Rooij H

MA3-010 was used in western blot to study the role of cell-cell interactions in the ligand-independent nuclear translocation of the glucocorticoid receptor

Thu Feb 01 00:00:00 UTC 1996

High levels of non-activated receptors in glucocorticoid-sensitive S49wt mouse lymphoma cells incubated with dexamethasone.

The Journal of steroid biochemistry and molecular biology

van den Berg JD,Smets LA,Hutchison KA,van Rooij H,van den Elshout MM

MA3-010 was used in western blot to investigate the glucocorticoid receptor status of murine S49 lymphoma cells exposed to dexamethasone

Sat Oct 01 00:00:00 UTC 1994

ATP-dependent protein refolding activity in reticulocyte lysate. Evidence for the participation of different chaperone components.

FEBS letters

Nimmesgern E,Hartl FU

MA3-010 was used in western blot to investigate the role of chaperone components for energy-dependent protein refolding activity

Mon Sep 27 00:00:00 UTC 1993

Bioinformatics

Protein Aliases: epididymis luminal secretory protein 52; epididymis secretory sperm binding protein Li 65p; Heat shock 86 kDa; heat shock 90kD protein 1, alpha; heat shock 90kD protein 1, alpha-like 4; heat shock 90kD protein, alpha-like 4; heat shock 90kDa protein 1, alpha; Heat shock protein; heat shock protein 1, alpha; heat shock protein 86; heat shock protein 90kDa alpha (cytosolic), class A member 1; heat shock protein 90kDa alpha family class A member 1; Heat shock protein family C member 1; Heat shock protein HSP 90-alpha; heat shock protein, 1; heat shock protein, 86 kDa 1; heat shock protein, 89 kDa; Heat shock protein90; HSP; HSP 86; HSP 90 alpha; HSP86; LAP-2; Lipopolysaccharide-associated protein 2; LPS-associated protein 2; Renal carcinoma antigen NY-REN-38; TSTA; Tumor-specific transplantation 86 kDa antigen

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Gene Aliases: 86kDa; 89kDa; AL024080; AL024147; BOS_20461; EL52; HEL-S-65p; hsp4; HSP86; Hsp86-1; Hsp89; HSP89A; Hsp90; HSP90A; HSP90AA1; HSP90N; HSPC1; HSPCA; HSPCAL1; HSPCAL4; HSPN; LAP-2; LAP2

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UniProt ID: (Human) P07900, (Bovine) Q76LV2, (Mouse) P07901, (Rat) P82995

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Entrez Gene ID: (Human) 3320, (Bovine) 281832, (Mouse) 15519, (Rat) 299331

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Function(s)

nucleotide binding UTP binding CTP binding mRNA binding protein tyrosine kinase activity protein binding ATP binding GTP binding ATPase activity sulfonylurea receptor binding protein kinase binding protein phosphatase binding MHC class II protein complex binding nitric-oxide synthase regulator activity TPR domain binding dATP binding identical protein binding protein homodimerization activity histone deacetylase binding ion channel binding poly(A) RNA binding GTPase binding Rho GDP-dissociation inhibitor binding unfolded protein binding

Process(es)

G2/M transition of mitotic cell cycle neuron migration skeletal muscle contraction mitochondrial transport receptor-mediated endocytosis response to unfolded protein signal transduction response to heat response to cold response to salt stress positive regulation of lamellipodium assembly cardiac muscle cell apoptotic process peptidyl-tyrosine phosphorylation regulation of protein ubiquitination positive regulation of protein import into nucleus, translocation Fc-gamma receptor signaling pathway involved in phagocytosis ERBB2 signaling pathway protein refolding regulation of protein complex assembly protein unfolding response to estrogen protein import into mitochondrial outer membrane positive regulation of nitric oxide biosynthetic process positive regulation of cell size response to antibiotic vascular endothelial growth factor receptor signaling pathway protein stabilization regulation of nitric-oxide synthase activity chaperone-mediated protein complex assembly positive regulation of cardiac muscle contraction chaperone-mediated autophagy regulation of cellular response to heat protein folding nitric oxide biosynthetic process response to stress positive regulation of cytotoxic T cell differentiation regulation of catalytic activity

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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HSP90 alpha Monoclonal Antibody (3B6)

HSP90 alpha Antibody (MA3-010) in ICC/IF

Product Details

MA3-010

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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