Invitrogen

HSP90 beta Monoclonal Antibody (H9010)

HSP90 beta Antibody in Immunocytochemistry (ICC/IF)

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HSP90 beta Antibody (37-9400) in ICC/IF

Immunofluorescence analysis of Hsp90 beta was done on 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Hsp90 beta (H9010) Mouse Monoclonal Antibody (Product # 37-9400) at 1:250 dilution in 0.1% BSA and incubated for 3 hours... View More

HSP90 beta Antibody in Immunohistochemistry (Paraffin) (IHC (P))

HSP90 beta Antibody in Western Blot (WB)

HSP90 beta Antibody in Immunocytochemistry, Immunoprecipitation (ICC/IF, IP)

HSP90 beta Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)

HSP90 beta Antibody in Western Blot, Immunocytochemistry, Immunoprecipitation (WB, ICC/IF, IP)

HSP90 beta Antibody in Immunohistochemistry (IHC)

HSP90 beta Antibody in Western Blot, Immunohistochemistry, Immunoprecipitation (WB, IHC, IP)

HSP90 beta Antibody in Western Blot, Immunoprecipitation (WB, IP)

HSP90 beta Antibody in ChIP Assay (ChIP)

Product Image Gallery

Product Details

37-9400

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

View 10 publications 10 publications

Immunohistochemistry (IHC)

View 5 publications 5 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20

Immunocytochemistry (ICC/IF)

1:250

View 6 publications 6 publications

ELISA (ELISA)

Assay-dependent

View 1 publication 1 publication

Immunoprecipitation (IP)

Assay-dependent

View 4 publications 4 publications

ChIP assay (ChIP)

View 1 publication 1 publication

Miscellaneous PubMed (MISC)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Chicken, Human, Mouse, Rabbit

Published species

Human, Mouse, Rat, Xenopus

Host/Isotype

Mouse / IgG2a, kappa

Class

Monoclonal

Type

Antibody

Clone

H9010

Immunogen

Recombinant human HSP90-beta

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

tissue culture supernatant

Contains

0.1% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2533349

Target Information

Heat shock proteins (HSP) are expressed in response to various biological stresses, including heat. HSP90 is a 90 kDa protein that is induced under stress conditions, but is also one of the most abundant cellular proteins found under non-stress conditions. HSP90 proteins are highly conserved molecular chaperones that have key roles in signal transduction, protein folding, protein degradation, and morphologic evolution. HSP90 proteins normally associate with other cochaperones and play important roles in folding newly synthesized proteins or stabilizing and refolding denatured proteins after stress. HSP90 has been found to be associated with a number of other intracellular proteins, including steroid receptors, actin, tubulin, aryl hydrocarbon (Ah) receptor, and some kinases.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

HSP90 promotes radioresistance of cervical cancer cells via reducing FBXO6-mediated CD147 polyubiquitination.

Cancer science

Song Q,Wen J,Li W,Xue J,Zhang Y,Liu H,Han J,Ning T,Lu Z

37-9400 was used in Immunohistochemistry to show that HSP90 promotes radioresistance of cervical cancer cells partially via reducing FBXO6 mediated CD147 polyubiquitination.

Fri Apr 01 00:00:00 UTC 2022

Cell-free DNA topology depends on its subcellular and cellular origins in cancer.

JCI insight

Malkin EZ,De Michino S,Lambie M,Gill R,Zhao Z,Rostami A,Arruda A,Minden MD,Bratman SV

37-9400 was used in Western Blotting to demonstrate that nuclear cfDNA is predominantly associated with nucleosomal particles and not EVs, while a substantial subset of mitochondrial cfDNA is membrane-protected and disproportionately derived from non-tumour cells.

Mon Oct 24 00:00:00 UTC 2022

Cell-free DNA topology depends on its subcellular and cellular origins in cancer.

JCI insight

Malkin EZ,De Michino S,Lambie M,Gill R,Zhao Z,Rostami A,Arruda A,Minden MD,Bratman SV

Mon Oct 24 00:00:00 UTC 2022

Heat shock protein HSP90 immunoexpression in equine endometrium during oestrus, dioestrus and anoestrus.

Anatomia, histologia, embryologia

Camacho Benítez A,Vasconcellos R,Lombide P,Viotti H,Pérez W,Cazales N,Cavestany D,Martin GB,Pedrana G

Published figure using HSP90 beta monoclonal antibody (Product # 37-9400) in Immunohistochemistry

Fri Jan 01 00:00:00 UTC 2021

Time-of-day specificity of anticancer drugs may be mediated by circadian regulation of the cell cycle.

Science advances

Lee Y,Fong SY,Shon J,Zhang SL,Brooks R,Lahens NF,Chen D,Dang CV,Field JM,Sehgal A

37-9400 was used in Western Blot to provide a powerful rationale for appropriate daily timing of anticancer drugs and suggest circadian regulation of the cell cycle within the tumor as an underlying mechanism.

Mon Feb 01 00:00:00 UTC 2021

Time-of-day specificity of anticancer drugs may be mediated by circadian regulation of the cell cycle.

Science advances

Lee Y,Fong SY,Shon J,Zhang SL,Brooks R,Lahens NF,Chen D,Dang CV,Field JM,Sehgal A

Mon Feb 01 00:00:00 UTC 2021

Maternal undernutrition during pregnancy and lactation increases transcription factors, ETV5 and GDNF, and alters regulation of apoptosis and heat shock proteins in the testis of adult offspring in the rat.

Reproduction, fertility, and development

Pedrana G,Larrañaga C,Diaz A,Viotti H,Lombide P,Cavestany D,Vickers MH,Martin GB,Sloboda DM

37-9400 was used in ELISA, Immunocytochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot to test whether changes in Sertoli cell transcription factors and germ cell heat shock proteins (HSPs) are linked to the effects of maternal undernutrition on male offspring fertility.

Sat May 01 00:00:00 UTC 2021

MAL2 mediates the formation of stable HER2 signaling complexes within lipid raft-rich membrane protrusions in breast cancer cells.

Cell reports

Jeong J,Shin JH,Li W,Hong JY,Lim J,Hwang JY,Chung JJ,Yan Q,Liu Y,Choi J,Wysolmerski J

37-9400 was used in Immunoprecipitation to show that MAL2-mediated lipid raft formation leads to HER2 plasma membrane retention and enhanced HER2 signaling in breast cancer cells.

Tue Dec 28 00:00:00 UTC 2021

Grp94 Regulates the Recruitment of Aneural AChR Clusters for the Assembly of Postsynaptic Specializations by Modulating ADF/Cofilin Activity and Turnover.

eNeuro

Chan ZC,Deng L,Lee CW

37-9400 was used in Western Blotting to investigatE the involvement of heat shock protein 90 (HSP90) family of stress proteins in neuromuscular junctions (NMJ) development.

Fri Jun 18 00:00:00 UTC 2021

Grp94 Regulates the Recruitment of Aneural AChR Clusters for the Assembly of Postsynaptic Specializations by Modulating ADF/Cofilin Activity and Turnover.

eNeuro

Chan ZC,Deng L,Lee CW

37-9400 was used in Western Blotting to investigatE the involvement of heat shock protein 90 (HSP90) family of stress proteins in neuromuscular junctions (NMJ) development.

Fri Jun 18 00:00:00 UTC 2021

Inhibition of ezrin causes PKCα-mediated internalization of erbb2/HER2 tyrosine kinase in breast cancer cells.

The Journal of biological chemistry

Jeong J,Choi J,Kim W,Dann P,Takyar F,Gefter JV,Friedman PA,Wysolmerski JJ

Published figure using HSP90 beta monoclonal antibody (Product # 37-9400) in Immunofluorescence

Fri Jan 18 00:00:00 UTC 2019

Effects of Pseudomonas aeruginosa on Microglial-Derived Extracellular Vesicle Biogenesis and Composition.

Pathogens (Basel, Switzerland)

Jones LB,Kumar S,Bell CR,Peoples VA,Crenshaw BJ,Coats MT,Scoffield JA,Rowe GC,Sims B,Matthews QL

Published figure using HSP90 beta monoclonal antibody (Product # 37-9400) in ELISA

Sat Dec 14 00:00:00 UTC 2019

Neural Precursor-Derived Pleiotrophin Mediates Subventricular Zone Invasion by Glioma.

Cell

Qin EY,Cooper DD,Abbott KL,Lennon J,Nagaraja S,Mackay A,Jones C,Vogel H,Jackson PK,Monje M

37-9400 was used in Immunohistochemistry to demonstrate a pathogenic role for neural precursor cell:glioma interactions and potential therapeutic targets to limit glioma invasion.

Thu Aug 24 00:00:00 UTC 2017

HER2 signaling regulates HER2 localization and membrane retention.

PloS one

Jeong J,Kim W,Kim LK,VanHouten J,Wysolmerski JJ

379400 was used in immunocytochemistry, immunoprecipitation, and western blot to explore how HER2 signaling contributes to the formation and maintenance of membrane protrusions

Wed Aug 30 00:00:00 UTC 2017

HER2 signaling regulates HER2 localization and membrane retention.

PloS one

Jeong J,Kim W,Kim LK,VanHouten J,Wysolmerski JJ

379400 was used in immunocytochemistry, immunoprecipitation, and western blot to explore how HER2 signaling contributes to the formation and maintenance of membrane protrusions

Wed Aug 30 00:00:00 UTC 2017

PMCA2 regulates HER2 protein kinase localization and signaling and promotes HER2-mediated breast cancer.

Proceedings of the National Academy of Sciences of the United States of America

Jeong J,VanHouten JN,Dann P,Kim W,Sullivan C,Yu H,Liotta L,Espina V,Stern DF,Friedman PA,Wysolmerski JJ

37-9400 was used in immunocytochemistry and western blot to study the interactions between PMCA2 and HER2 in breast cancer cells

Tue Jan 19 00:00:00 UTC 2016

PMCA2 regulates HER2 protein kinase localization and signaling and promotes HER2-mediated breast cancer.

Proceedings of the National Academy of Sciences of the United States of America

Jeong J,VanHouten JN,Dann P,Kim W,Sullivan C,Yu H,Liotta L,Espina V,Stern DF,Friedman PA,Wysolmerski JJ

37-9400 was used in immunocytochemistry and western blot to study the interactions between PMCA2 and HER2 in breast cancer cells

Tue Jan 19 00:00:00 UTC 2016

PMCA2 regulates HER2 protein kinase localization and signaling and promotes HER2-mediated breast cancer.

Proceedings of the National Academy of Sciences of the United States of America

Jeong J,VanHouten JN,Dann P,Kim W,Sullivan C,Yu H,Liotta L,Espina V,Stern DF,Friedman PA,Wysolmerski JJ

37-9400 was used in immunocytochemistry and western blot to study the interactions between PMCA2 and HER2 in breast cancer cells

Tue Jan 19 00:00:00 UTC 2016

A C-terminal HSP90 inhibitor restores glucocorticoid sensitivity and relieves a mouse allograft model of Cushing disease.

Nature medicine

Riebold M,Kozany C,Freiburger L,Sattler M,Buchfelder M,Hausch F,Stalla GK,Paez-Pereda M

37-9400 was used in western blot to investigate how HSP90 affects the glucocorticoid receptor in corticotroph cells.

Sun Mar 01 00:00:00 UTC 2015

The Inhibition of Heat Shock Protein 90 Facilitates the Degradation of Poly-Alanine Expanded Poly (A) Binding Protein Nuclear 1 via the Carboxyl Terminus of Heat Shock Protein 70-Interacting Protein.

PloS one

Shi C,Huang X,Zhang B,Zhu D,Luo H,Lu Q,Xiong WC,Mei L,Luo S

37-9400 was used in immunocytochemistry and western blot to discuss factors that contribute to the onset and progression of oculopharyngeal muscular dystrophy.

Tue Jun 07 00:00:00 UTC 2016

The Inhibition of Heat Shock Protein 90 Facilitates the Degradation of Poly-Alanine Expanded Poly (A) Binding Protein Nuclear 1 via the Carboxyl Terminus of Heat Shock Protein 70-Interacting Protein.

PloS one

Shi C,Huang X,Zhang B,Zhu D,Luo H,Lu Q,Xiong WC,Mei L,Luo S

37-9400 was used in immunocytochemistry and western blot to discuss factors that contribute to the onset and progression of oculopharyngeal muscular dystrophy.

Tue Jun 07 00:00:00 UTC 2016

Histone Deacetylases Positively Regulate Transcription through the Elongation Machinery.

Cell reports

Greer CB,Tanaka Y,Kim YJ,Xie P,Zhang MQ,Park IH,Kim TH

Published figure using HSP90 beta monoclonal antibody (Product # 37-9400) in ChIP assay

Tue Nov 17 00:00:00 UTC 2015

Heat shock protein 90β stabilizes focal adhesion kinase and enhances cell migration and invasion in breast cancer cells.

Experimental cell research

Xiong X,Wang Y,Liu C,Lu Q,Liu T,Chen G,Rao H,Luo S

37-9400 was used in immunocytochemistry and western blot to study the role of heat shock protein 90beta in cell migration and invasion of breast cancer cells

Fri Aug 01 00:00:00 UTC 2014

Heat shock protein 90β stabilizes focal adhesion kinase and enhances cell migration and invasion in breast cancer cells.

Experimental cell research

Xiong X,Wang Y,Liu C,Lu Q,Liu T,Chen G,Rao H,Luo S

37-9400 was used in immunocytochemistry and western blot to study the role of heat shock protein 90beta in cell migration and invasion of breast cancer cells

Fri Aug 01 00:00:00 UTC 2014

Heat shock protein 90β stabilizes focal adhesion kinase and enhances cell migration and invasion in breast cancer cells.

Experimental cell research

Xiong X,Wang Y,Liu C,Lu Q,Liu T,Chen G,Rao H,Luo S

37-9400 was used in immunocytochemistry and western blot to study the role of heat shock protein 90beta in cell migration and invasion of breast cancer cells

Fri Aug 01 00:00:00 UTC 2014

Dissecting cellular responses to irradiation via targeted disruptions of the ATM-CHK1-PP2A circuit.

Cell cycle (Georgetown, Tex.)

Palii SS,Cui Y,Innes CL,Paules RS

37-9400 was used in Western Blotting to indicate that dual targeting of ATM and CHK1 functionalities disrupts the compensatory response to DNA damage and could be exploited for developing efficient anti-neoplastic treatments.

Mon Apr 01 00:00:00 UTC 2013

Proteomic identification of putative biomarkers of radiotherapy resistance: a possible role for the 26S proteasome?

Neoplasia (New York, N.Y.)

Smith L,Qutob O,Watson MB,Beavis AW,Potts D,Welham KJ,Garimella V,Lind MJ,Drew PJ,Cawkwell L

Sun Nov 01 00:00:00 UTC 2009

HSP90 beta regulates rapsyn turnover and subsequent AChR cluster formation and maintenance.

Neuron

Luo S,Zhang B,Dong XP,Tao Y,Ting A,Zhou Z,Meixiong J,Luo J,Chiu FC,Xiong WC,Mei L