Proteintech

HSPA2 Polyclonal Antibody

View all (19) HSPA2 antibodies

Questions & Answers

Questions & Answers

Antibody Testing Data (17)

HSPA2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

HSPA2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 17

HSPA2 Antibody (12797-1-AP) in IHC (P)

Immunohistochemistry of paraffin-embedded human ovary tumor tissue slide using 12797-1-AP (HSPA2 antibody) at dilution of 1:50 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

HSPA2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

HSPA2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

HSPA2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

HSPA2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

HSPA2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

HSPA2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

HSPA2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

HSPA2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

HSPA2 Antibody in Western Blot (WB)

HSPA2 Antibody in Western Blot (WB)

HSPA2 Antibody in Western Blot (WB)

HSPA2 Antibody in Western Blot (WB)

HSPA2 Antibody in Western Blot (WB)

HSPA2 Antibody in Western Blot (WB)

HSPA2 Antibody in Western Blot (WB)

HSPA2 Antibody in Flow Cytometry (Flow)

HSPA2 Antibody in Immunoprecipitation (IP)

Product Image Gallery

HSPA2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human ovary tumor tissue slide using 12797-1-AP (HSPA2 antibody) at dilution of 1:50 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

HSPA2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human ovary tumor tissue slide using 12797-1-AP (HSPA2 antibody) at dilution of 1:50 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

HSPA2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded mouse ovary tissue slide using 12797-1-AP (HSPA2 antibody) at dilution of 1:50 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

HSPA2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded mouse ovary tissue slide using 12797-1-AP (HSPA2 antibody) at dilution of 1:50 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

HSPA2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human prostate hyperplasia using 12797-1-AP (HSPA2 antibody) at dilution of 1:50 (under 40x lens).

HSPA2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human prostate hyperplasia using 12797-1-AP (HSPA2 antibody) at dilution of 1:50 (under 10x lens).

HSPA2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human testis using 12797-1-AP (HSPA2 antibody) at dilution of 1:50 (under 40x lens).

HSPA2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human testis using 12797-1-AP (HSPA2 antibody) at dilution of 1:50 (under 10x lens).

HSPA2 Antibody in Western Blot (WB) — Human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 12797-1-AP (HSPA2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

HSPA2 Antibody in Western Blot (WB) — Human ileum tissue were subjected to SDS PAGE followed by western blot with 12797-1-AP (HSPA2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

HSPA2 Antibody in Western Blot (WB) — Human kidney tissue were subjected to SDS PAGE followed by western blot with 12797-1-AP (HSPA2 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours.

HSPA2 Antibody in Western Blot (WB) — Human brain tissue were subjected to SDS PAGE followed by western blot with 12797-1-AP (HSPA2 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours.

HSPA2 Antibody in Western Blot (WB) — HeLa cells were subjected to SDS PAGE followed by western blot with 12797-1-AP (HSPA2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

HSPA2 Antibody in Western Blot (WB) — A431 cells were subjected to SDS PAGE followed by western blot with 12797-1-AP (HSPA2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

HSPA2 Antibody in Western Blot (WB) — MCF7 cells were subjected to SDS PAGE followed by western blot with 12797-1-AP (HSPA2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

HSPA2 Antibody in Flow Cytometry (Flow) — 1X10^6 HeLa cells were stained with 0.2ug HSPA2 antibody (12797-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L) with dilution 1:1000.

HSPA2 Antibody in Immunoprecipitation (IP) — IP result of anti-HSPA2 (IP:12797-1-AP, 4ug; Detection:12797-1-AP 1:1000) with mouse skeletal muscle tissue lysate 4000ug.

Product Details

12797-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 7 publications 7 publications

Immunohistochemistry (IHC)

-

View 6 publications 6 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

-

Immunocytochemistry (ICC/IF)

-

View 2 publications 2 publications

Flow Cytometry (Flow)

0.2 µg/1x10^6 cells

-

Immunoprecipitation (IP)

0.5-4.0 µg

View 1 publication 1 publication

Miscellaneous PubMed (MISC)

-

View 1 publication 1 publication

Not applicable (N/A)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

HSPA2 Fusion Protein Ag3539 (290-639 aa encoded by BC036107)

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.14 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

This antibody well recognized the endogenous HSPA4 protein in testis and multiple cell lines.

Immunogen sequence: LYEGVDFYT SITRARFEEL NADLFRGTLE PVEKALRDAK LDKGQIQEIV LVGGSTRIPK IQKLLQDFFN GKELNKSINP DEAVAYGAAV QAAILIGDKS ENVQDLLLLD VTPLSLGIET AGGVMTPLIK RNTTIPTKQT QTFTTYSDNQ SSVLVQVYEG ERAMTKDNNL LGKFDLTGIP PAPRGVPQIE VTFDIDANGI LNVTAADKST GKENKITITN DKGRLSKDDI DRMVQEAERY KSEDEANRDR VAAKNALESY TYNIKQTVED EKLRGKISEQ DKNKILDKCQ EVINWLDRNQ MAEKDEYEHK QKELERVCNP IISKLYQGGP GGGSGGGGSG ASGGPTIEEV D (290-639 aa encoded by BC036107)

Target Information

In cooperation with other chaperones, Hsp70s stabilize preexistent proteins against aggregation and mediate the folding of newly translated polypeptides in the cytosol as well as within organelles. These chaperones participate in all these processes through their ability to recognize nonnative conformations of other proteins. They bind extended peptide segments with a net hydrophobic character exposed by polypeptides during translation and membrane translocation, or following stress-induced damage.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Non-invasive activation of intratumoural gene editing for improved adoptive T-cell therapy in solid tumours.

Nature nanotechnology

Chen X,Wang S,Chen Y,Xin H,Zhang S,Wu D,Xue Y,Zha M,Li H,Li K,Gu Z,Wei W,Ping Y

Tue Aug 01 00:00:00 UTC 2023

Sleeve gastrectomy improves lipid dysmetabolism by downregulating the USP20-HSPA2 axis in diet-induced obese mice.

Frontiers in endocrinology

Zhang W,Shi B,Li S,Liu Z,Li S,Dong S,Cheng Y,Zhu J,Zhang G,Zhong M

Tue Jan 17 00:00:00 UTC 2023

Sleeve gastrectomy improves lipid dysmetabolism by downregulating the USP20-HSPA2 axis in diet-induced obese mice.

Frontiers in endocrinology

Zhang W,Shi B,Li S,Liu Z,Li S,Dong S,Cheng Y,Zhu J,Zhang G,Zhong M

Tue Jan 17 00:00:00 UTC 2023

Sleeve gastrectomy improves lipid dysmetabolism by downregulating the USP20-HSPA2 axis in diet-induced obese mice.

Frontiers in endocrinology

Zhang W,Shi B,Li S,Liu Z,Li S,Dong S,Cheng Y,Zhu J,Zhang G,Zhong M

Tue Jan 17 00:00:00 UTC 2023

Sleeve gastrectomy improves lipid dysmetabolism by downregulating the USP20-HSPA2 axis in diet-induced obese mice.

Frontiers in endocrinology

Zhang W,Shi B,Li S,Liu Z,Li S,Dong S,Cheng Y,Zhu J,Zhang G,Zhong M

Tue Jan 17 00:00:00 UTC 2023

Sleeve gastrectomy improves lipid dysmetabolism by downregulating the USP20-HSPA2 axis in diet-induced obese mice.

Frontiers in endocrinology

Zhang W,Shi B,Li S,Liu Z,Li S,Dong S,Cheng Y,Zhu J,Zhang G,Zhong M

Tue Jan 17 00:00:00 UTC 2023

Sleeve gastrectomy improves lipid dysmetabolism by downregulating the USP20-HSPA2 axis in diet-induced obese mice.

Frontiers in endocrinology

Zhang W,Shi B,Li S,Liu Z,Li S,Dong S,Cheng Y,Zhu J,Zhang G,Zhong M

Tue Jan 17 00:00:00 UTC 2023

RNF144A functions as a tumor suppressor in breast cancer through ubiquitin ligase activity-dependent regulation of stability and oncogenic functions of HSPA2.

Cell death and differentiation

Yang YL,Zhang Y,Li DD,Zhang FL,Liu HY,Liao XH,Xie HY,Lu Q,Zhang L,Hong Q,Dong WJ,Li DQ,Shao ZM

Sun Mar 01 00:00:00 UTC 2020

Melatonin protects the mouse testis against heat-induced damage.

Molecular human reproduction

Zhang P,Zheng Y,Lv Y,Li F,Su L,Qin Y,Zeng W

Sat Feb 29 00:00:00 UTC 2020

Melatonin protects the mouse testis against heat-induced damage.

Molecular human reproduction

Zhang P,Zheng Y,Lv Y,Li F,Su L,Qin Y,Zeng W

Sat Feb 29 00:00:00 UTC 2020

Various Anti-HSPA2 Antibodies Yield Different Results in Studies on Cancer-Related Functions of Heat Shock Protein A2.

International journal of molecular sciences

Scieglinska D,Sojka DR,Gogler-Pigłowska A,Chumak V,Krawczyk Z

Tue Jun 16 00:00:00 UTC 2020

Various Anti-HSPA2 Antibodies Yield Different Results in Studies on Cancer-Related Functions of Heat Shock Protein A2.

International journal of molecular sciences

Scieglinska D,Sojka DR,Gogler-Pigłowska A,Chumak V,Krawczyk Z

Tue Jun 16 00:00:00 UTC 2020

Circular RNAs from BOULE play conserved roles in protection against stress-induced fertility decline.

Science advances

Gao L,Chang S,Xia W,Wang X,Zhang C,Cheng L,Liu X,Chen L,Shi Q,Huang J,Xu EY,Shan G

Sun Nov 01 00:00:00 UTC 2020

Nuclear localization of EIF4G3 suggests a role for the XY body in translational regulation during spermatogenesis in mice.

Biology of reproduction

Hu J,Sun F,Handel MA

Mon Jan 01 00:00:00 UTC 2018

Nuclear localization of EIF4G3 suggests a role for the XY body in translational regulation during spermatogenesis in mice.

Biology of reproduction

Hu J,Sun F,Handel MA

Mon Jan 01 00:00:00 UTC 2018

RPL10L Is Required for Male Meiotic Division by Compensating for RPL10 during Meiotic Sex Chromosome Inactivation in Mice.

Current biology : CB

Jiang L,Li T,Zhang X,Zhang B,Yu C,Li Y,Fan S,Jiang X,Khan T,Hao Q,Xu P,Nadano D,Huleihel M,Lunenfeld E,Wang PJ,Zhang Y,Shi Q

Mon May 22 00:00:00 UTC 2017

RPL10L Is Required for Male Meiotic Division by Compensating for RPL10 during Meiotic Sex Chromosome Inactivation in Mice.

Current biology : CB

Jiang L,Li T,Zhang X,Zhang B,Yu C,Li Y,Fan S,Jiang X,Khan T,Hao Q,Xu P,Nadano D,Huleihel M,Lunenfeld E,Wang PJ,Zhang Y,Shi Q

Mon May 22 00:00:00 UTC 2017

Cigarette smoke-induced cell cycle arrest in spermatocytes [GC-2spd(ts)] is mediated through crosstalk between Ahr-Nrf2 pathway and MAPK signaling.

Journal of molecular cell biology

Esakky P,Hansen DA,Drury AM,Moley KH

Sun Feb 01 00:00:00 UTC 2015

Cigarette smoke-induced cell cycle arrest in spermatocytes [GC-2spd(ts)] is mediated through crosstalk between Ahr-Nrf2 pathway and MAPK signaling.

Journal of molecular cell biology

Esakky P,Hansen DA,Drury AM,Moley KH

Sun Feb 01 00:00:00 UTC 2015

Molecular analysis of cell type-specific gene expression profile during mouse spermatogenesis by laser microdissection and qRT-PCR.

Reproductive sciences (Thousand Oaks, Calif.)

Esakky P,Hansen DA,Drury AM,Moley KH

Fri Mar 01 00:00:00 UTC 2013

Molecular analysis of cell type-specific gene expression profile during mouse spermatogenesis by laser microdissection and qRT-PCR.

Reproductive sciences (Thousand Oaks, Calif.)

Esakky P,Hansen DA,Drury AM,Moley KH

Fri Mar 01 00:00:00 UTC 2013

Molecular analysis of cell type-specific gene expression profile during mouse spermatogenesis by laser microdissection and qRT-PCR.

Reproductive sciences (Thousand Oaks, Calif.)

Esakky P,Hansen DA,Drury AM,Moley KH

Fri Mar 01 00:00:00 UTC 2013

Molecular analysis of cell type-specific gene expression profile during mouse spermatogenesis by laser microdissection and qRT-PCR.

Reproductive sciences (Thousand Oaks, Calif.)

Esakky P,Hansen DA,Drury AM,Moley KH

Fri Mar 01 00:00:00 UTC 2013

Mutation of Eif4g3, encoding a eukaryotic translation initiation factor, causes male infertility and meiotic arrest of mouse spermatocytes.

Development (Cambridge, England)

Sun F,Palmer K,Handel MA

Sat May 01 00:00:00 UTC 2010

Mutation of Eif4g3, encoding a eukaryotic translation initiation factor, causes male infertility and meiotic arrest of mouse spermatocytes.

Development (Cambridge, England)

Sun F,Palmer K,Handel MA

Sat May 01 00:00:00 UTC 2010

Mutation of Eif4g3, encoding a eukaryotic translation initiation factor, causes male infertility and meiotic arrest of mouse spermatocytes.

Development (Cambridge, England)

Sun F,Palmer K,Handel MA

Sat May 01 00:00:00 UTC 2010

Mutation of Eif4g3, encoding a eukaryotic translation initiation factor, causes male infertility and meiotic arrest of mouse spermatocytes.

Development (Cambridge, England)

Sun F,Palmer K,Handel MA

Sat May 01 00:00:00 UTC 2010

Bioinformatics

Protein Aliases: Heat shock 70 kDa protein 2; heat shock 70kD protein 2; heat shock 70kDa protein 2; Heat shock protein; Heat shock protein 70.2; heat shock protein alpha 2; Heat shock protein family A member 2; heat shock protein, 70 kDa 2; Heat shock-related 70 kDa protein 2; HSP; HST; Testis-specific heat shock protein-related; testis-specific heat shock protein-related gene hst70

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Gene Aliases: 70kDa; Hcp70.2; HSP70-2; HSP70-3; HSP70.2; HSP70A2; HSPA2; Hspt70; Hst70

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UniProt ID: (Human) P54652, (Rat) P14659, (Mouse) P17156

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Entrez Gene ID: (Human) 3306, (Rat) 60460, (Mouse) 15512

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protein binding ATP binding enzyme binding unfolded protein binding glycolipid binding nucleotide binding

response to unfolded protein male meiosis male meiosis I spermatogenesis spermatid development response to heat response to cold positive regulation of cyclin-dependent protein serine/threonine kinase activity involved in G2/M transition of mitotic cell cycle protein refolding synaptonemal complex disassembly negative regulation of inclusion body assembly positive regulation of calcium-transporting ATPase activity positive regulation of protein phosphorylation multicellular organism development

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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