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Invitrogen
HSPA4 Polyclonal Antibody
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HSPA4 Antibody (PA5-17053) in WB
Western blot was performed using Anti-HSPA4 Rabbit Polyclonal Antibody (Product # PA5-17053) and a 110kDa band corresponding to HSPA4 was observed across cell lines and tissues tested. Whole cell extracts (30 µg lysate) of Hep G2 (Lane 1), HeLa (Lane 2), HEK-293 (Lane 3), A549 (Lane 4), COS-7 (Lane 5), Mouse Testis (Lane 6) and Rat Ovary (Lane 7) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blot... View More
Product Details
PA5-17053
Product Specifications
Species Reactivity
Human,
Mouse,
Non-human primate,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide corresponding to human HSPA4
Conjugate
Form
Liquid
Purification
Antigen affinity chromatography
Storage buffer
0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_10979480
Product Specific Information
It is not recommended to aliquot this antibody.
Target Information
The HSP 70 family is composed of four highly conserved proteins: HSP 70, HSC 70, GRP 75 and GRP 78. HSP 70 expression is strongly induced in response to heat stress. HSP 70 and HSC 70 play key roles in the cytosolic endoplasmic reticulum and mitochondrial import machinery and are found in both the cytosol and nucleus of mammalian cells. GRP 78 is localized in the endoplasmic reticulum, where it receives imported secretory proteins and is involved in the folding and translocation of nascent peptide chains. GRP 75 expression is restricted to the mitochondrial matrix and aids in the translocation and folding of nascent polypeptide chains of both nuclear and mitochondrial origin. It has been postulated that members of the HSP 70 family act as force-generating motors, relying on the hydrolysis of ATP for their activity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: epididymis secretory sperm binding protein Li 5a; Heat shock 70 kDa protein 4; Heat shock 70-related protein APG-2; heat shock 70kD protein 4; heat shock 70kDa protein 4; Heat shock protein; Heat shock protein family H member 2; heat shock protein, 110 kDa; hsp70 RY; HSP70RY; Ischemia responsive 94 kDa protein
Gene Aliases: 70kDa; AI317151; APG-2; APG2; HEL-S-5a; HS24/P52; Hsp110; Hsp70; Hsp70RY; HSPA4; HSPH2; Irp94; mKIAA4025; RY
UniProt ID: (Human) P34932, (Rat) O88600
Entrez Gene ID: (Human) 3308, (Mouse) 15525, (Rat) 266759
negative regulation of protein phosphorylation
response to heat
positive regulation of gene expression
negative regulation of gene expression
positive regulation of protein binding
negative regulation of apoptotic process
negative regulation of DNA binding
protein import into mitochondrial outer membrane
chaperone-mediated protein complex assembly
negative regulation of cell death
response to unfolded protein
kidney development
positive regulation of angiogenesis
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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