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Invitrogen
HSPE1 Recombinant Rabbit Monoclonal Antibody (0H0X6)
FIGURE: 1 / 10
HSPE1 Antibody (MA5-35686) in ICC/IF
Immunofluorescence analysis of HSPE1 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with HSPE1 Recombinant Rabbit Monoclonal Antibody (ARC1411) (Product # MA5-35686) at 1:100 in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor™ Plu... View More
Product Details
MA5-35686
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
0H0X6
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 50-150 of human HSPE1/HSP10/CPN10 (P61604)
Conjugate
Form
Liquid
Concentration
0.43 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol, 0.05% BSA
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2849586
Product Specific Information
Immunogen sequence: GSGSKGKGGE IQPVSVKVGD KVLLPEYGGT KVVLDDKDYF LFRDGDILGK YVD
Target Information
The heat shock proteins (HSPs) comprise a group of highly conserved, abundantly expressed proteins with diverse functions, including the assembly and sequestering of multiprotein complexes, transportation of nascent polypeptide chains across cellular membranes and regulation of protein folding. Heat shock proteins (also known as molecular chaperones) fall into six general families: HSP 90, HSP 70, HSP 60, the low molecular weight HSPs, the immunophilins and the HSP 110 family. The low molecular weight family includes HSP 10, HSP 20, HSP 27 (Heme Oxygenase 1), HSP 32 and HSP 40. Chaperonins are ubiquitous, indispensable proteins that facilitate protein folding in an ATP-dependent manner, enhancing the yield of properly folded substrate protein under conditions where spontaneous folding does not occur. Chaperonins are typified by the E. coli heat-shock proteins GroEL and GroES (HSP 10). HSP 10 is a heptameric ring of identical 10 kDa subunits that binds to each end of GroEL to form a symmetric, functional heterodimer.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 10 kDa chaperonin; 10 kDa heat shock protein, mitochondrial; 10kDa Chaperonin; CH10; Chaperonin 10; Cpn 10; CPN10; early pregnancy factor; Early-pregnancy factor; EPF; Heat shock 10 kD protein 1 (chaperonin 10); heat shock 10 kDa protein 1 (chaperonin 10); heat shock 10kD protein 1 (chaperonin 10); heat shock 10kDa protein 1; Heat shock protein family E member 1; HSP; Hsp10; mitochondrial chaperonin 10
Gene Aliases: 10kDa; CPN10; EPF; GROES; HSP10; HSPE1; mt-cpn10
UniProt ID: (Human) Q53X54, (Mouse) Q64433
Entrez Gene ID: (Human) 3336, (Rat) 25462, (Mouse) 15528
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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