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Invitrogen
HSPH1 Monoclonal Antibody (3D10)
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FIGURE: 1 / 8
HSPH1 Antibody (MA5-36231) in ICC/IF
Immunocytochemistry analysis of HSPH1 in MCF7 cells. Antigen retrieval was performed with enzyme antigen retrieval (15 min). Samples were blocked with 10% goat serum and incubated in HSPH1 monoclonal antibody (Product # MA5-36231) at a dilution of 2 µg/mL (overnight, 4°C), followed by DyLight 488 conjugated goat anti-mouse IgG (30 min, 37°C) and DAPI at a dilution of 1:100.
Product Details
MA5-36231
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
3D10
Immunogen
E. coli-derived human Hsp105 recombinant protein (Position: Y653-D858).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2884065
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: human Caco-2 whole cell, human K562 whole cell, human A549 whole cell, human HepG2 whole cell, human PANC-1 whole cell, human SGC-7901 whole cell. IHC: human lung cancer tissue, human mammary cancer tissue, human intestinal cancer tissue. ICC/IF: A431 cell, MCF7 cell. Flow: A431 cell, HepG2 cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Target Information
HSPH1 prevents the aggregation of denatured proteins in cells under severe stress, on which the ATP levels decrease markedly. This protein inhibits HSPA8/HSC70 ATPase and chaperone activities.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Antigen NY-CO-25; heat shock 105kD alpha; heat shock 105kD beta; heat shock 105kDa/110kDa protein 1; Heat shock 110 kDa protein; Heat shock protein; Heat shock protein 105 kDa; Heat shock protein family H member 1; HSP; NY CO 25
Gene Aliases: HSP105; HSP105A; HSP105B; HSP110; HSPH1; KIAA0201; NY-CO-25
UniProt ID: (Human) Q92598
Entrez Gene ID: (Human) 10808
receptor-mediated endocytosis
response to unfolded protein
positive regulation of MHC class I biosynthetic process
positive regulation of transcription from RNA polymerase II promoter
chaperone mediated protein folding requiring cofactor
positive regulation of NK T cell activation
positive regulation of protein tyrosine kinase activity
regulation of cellular response to heat
negative regulation of establishment of protein localization to mitochondrion
negative regulation of intrinsic apoptotic signaling pathway in response to hydrogen peroxide
negative regulation of p38MAPK cascade
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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