Proteintech

HUWE1 Polyclonal Antibody

HUWE1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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HUWE1 Antibody (19430-1-AP) in IHC (P)

Immunohistochemistry of paraffin-embedded human colon cancer tissue slide using 19430-1-AP (HUWE1 antibody) at dilution of 1:1000 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

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Product Details

19430-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 7 publications 7 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:500-1:2,000

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Immunoprecipitation (IP)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

HUWE1 Fusion Protein Ag13763 (3983-4374 aa encoded by BC002602)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.13 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: AVLVDYIRV LDFDVKRKYF RQELERLDEG LRKEDMAVHV LRDHVFEDSY RELHRKSPEE MKNRLYIVFE GEEGQDAGGL LREWYMIISR EMFNPMYALF RTSPGDRVTY TINPSSHCNP NHLSYFKFVG RIVAKAVYDN RLLECYFTRS FYKHILGKSV RYTDMESEDY HFYQGLVYLL ENDVSTLGYD LTFSTEVQEF GVCEVRDLKP NGANILVTEE NKKEYVHLVC QMRMTGAIRK QLAAFLEGFY EIIPKRLISI FTEQELELLI SGLPTIDIDD LKSNTEYHKY QSNSIQIQWF WRALRSFDQA DRAKFLQFVT GTSKVPLQGF AALEGMNGIQ KFQIHRDDRS TDRLPSAHTC FNQLDLPAYE SFEKLRHMLL LAIQECSEGF GLA (3983-4374 aa encoded by BC002602)

Target Information

SUFU encodes a component of the Sonic hedgehog (SHH; MIM 600725)/Patched (PTCH; MIM 601309) signaling pathway. Mutations in genes encoding components of this pathway are deleterious for normal development and are associated with cancer-predisposing syndromes (e.g., HPE3, MIM 142945; BCNS, MIM 109400; and GCPS, MIM 175700).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

The role of USP7 in the Shoc2-ERK1/2 signaling axis and Noonan-like syndrome with loose anagen hair.

Journal of cell science

Wilson P,Abdelmoti L,Norcross R,Jang ER,Palayam M,Galperin E

Mon Nov 01 00:00:00 UTC 2021

The role of USP7 in the Shoc2-ERK1/2 signaling axis and Noonan-like syndrome with loose anagen hair.

Journal of cell science

Wilson P,Abdelmoti L,Norcross R,Jang ER,Palayam M,Galperin E

Mon Nov 01 00:00:00 UTC 2021

WNK1 is an assembly factor for the human ER membrane protein complex.

Molecular cell

Pleiner T,Hazu M,Tomaleri GP,Januszyk K,Oania RS,Sweredoski MJ,Moradian A,Guna A,Voorhees RM

Thu Jul 01 00:00:00 UTC 2021

Genomic gain of RRS1 promotes hepatocellular carcinoma through reducing the RPL11-MDM2-p53 signaling.

Science advances

Cao P,Yang A,Li P,Xia X,Han Y,Zhou G,Wang R,Yang F,Li Y,Zhang Y,Cui Y,Ji H,Lu L,He F,Zhou G

Sun Aug 01 00:00:00 UTC 2021

HUWE1 promotes EGFR ubiquitination and degradation to protect against renal tubulointerstitial fibrosis.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Zhu Q,Dong H,Bukhari AA,Zhao A,Li M,Sun Y,Zhang X,Cao C,Su D,Liang X

Sun Mar 01 00:00:00 UTC 2020

NEDD4L-mediated LTF protein degradation limits ferroptosis.

Biochemical and biophysical research communications

Wang Y,Liu Y,Liu J,Kang R,Tang D

Thu Oct 22 00:00:00 UTC 2020

Ubiquitination and inhibition of glycine receptor by HUWE1 in spinal cord dorsal horn.

Neuropharmacology

Zhang ZY,Guo Z,Li HL,He YT,Duan XL,Suo ZW,Yang X,Hu XD

Mon Apr 01 00:00:00 UTC 2019

Ubiquitination and inhibition of glycine receptor by HUWE1 in spinal cord dorsal horn.

Neuropharmacology

Zhang ZY,Guo Z,Li HL,He YT,Duan XL,Suo ZW,Yang X,Hu XD

Mon Apr 01 00:00:00 UTC 2019

Derlin-1 promotes ubiquitylation and degradation of the epithelial Na⁺ channel, ENaC.

Journal of cell science

You H,Ge Y,Zhang J,Cao Y,Xing J,Su D,Huang Y,Li M,Qu S,Sun F,Liang X

Wed Mar 15 00:00:00 UTC 2017

Derlin-1 promotes ubiquitylation and degradation of the epithelial Na⁺ channel, ENaC.

Journal of cell science

You H,Ge Y,Zhang J,Cao Y,Xing J,Su D,Huang Y,Li M,Qu S,Sun F,Liang X

Wed Mar 15 00:00:00 UTC 2017

Bioinformatics

Protein Aliases: ARF-binding protein 1; ARF-BP1; BJ-HCC-24 tumor antigen; E3 ubiquitin-protein ligase HUWE1; E3Histone; HECT domain protein LASU1; HECT, UBA and WWE domain-containing protein 1; HECT-type E3 ubiquitin transferase HUWE1; HectH9; Homologous to E6AP carboxyl terminus homologous protein 9; Large structure of UREB1; LASU1; Mcl-1 ubiquitin ligase; Mcl-1 ubiquitin ligase E3; Mule; RP3-339A18.4; upstream regulatory element binding protein 1; Upstream regulatory element-binding protein 1; URE-B1; URE-binding protein 1

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Gene Aliases: 5430439H10Rik; ARF-BP1; AU041296; C430014N20Rik; C80292; Gm1718; HECTH9; HSPC272; HUWE1; Ib772; KIAA0312; KIAA1578; LASU1; MULE; URE-B1; UREB1

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UniProt ID: (Human) Q7Z6Z7, (Mouse) Q7TMY8

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Entrez Gene ID: (Human) 10075, (Mouse) 59026

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Function(s)

DNA binding ubiquitin-protein transferase activity protein binding ligase activity poly(A) RNA binding UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity ribosomal S6-glutamic acid ligase activity coenzyme F420-0 gamma-glutamyl ligase activity coenzyme F420-2 alpha-glutamyl ligase activity protein-glycine ligase activity protein-glycine ligase activity, initiating protein-glycine ligase activity, elongating tubulin-glycine ligase activity protein-glutamic acid ligase activity tubulin-glutamic acid ligase activity

Process(es)

protein polyubiquitination base-excision repair protein monoubiquitination histone ubiquitination cell differentiation protein ubiquitination involved in ubiquitin-dependent protein catabolic process positive regulation of protein targeting to mitochondrion DNA repair cellular response to DNA damage stimulus

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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HUWE1 Polyclonal Antibody

HUWE1 Antibody (19430-1-AP) in IHC (P)

Product Details

19430-1-AP

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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