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Invitrogen
HYI Polyclonal Antibody
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FIGURE: 1 / 6
HYI Antibody (PA5-144039) in ICC/IF
Immunocytochemistry analysis of HYI in A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. Samples were then incubated in HYI Polyclonal antibody (Product # PA5-144039) using a dilution of 5 μg/mL. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for t... View More
Product Details
PA5-144039
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived human HYI recombinant protein (Position: E46-Q277).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
0.01mg sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_3075253
Product Specific Information
Adding 0.2 mL of distilled water will yield a concentration of 500 µg/mL.
Positive Control - WB: rat liver tissue, rat kidney tissue, mouse liver tissue. IHC: human melanomar tissue, human rectal cancer tissue. ICC/IF: A431 cell. Flow: THP-1 cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Target Information
Catalyzes the reversible isomerization between hydroxypyruvate and 2-hydroxy-3-oxopropanoate (also termed tartronate semialdehyde).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Endothelial cell apoptosis protein E-CE1; hydroxypyruvate isomerase; hydroxypyruvate isomerase homolog; Putative hydroxypyruvate isomerase
Gene Aliases: 2700033B16Rik; 6430559E15Rik; HT036; HYI; RGD1561416; SB156
UniProt ID: (Human) Q5T013, (Mouse) Q8R1F5
Entrez Gene ID: (Human) 81888, (Mouse) 68180, (Rat) 500536
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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