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Product Details
HYPE-101AP
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide corresponding to amino acids within region 400-450 on mouse Protein adenylyltransferase FICD.
Conjugate
Form
Liquid
Concentration
0.5-1.5 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
proprietary buffer with 30% glycerol
Contains
0.01% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
Target Information
Protein that can both mediate the addition of adenosine 5'-monophosphate (AMP) to specific residues of target proteins (AMPylation), and the removal of the same modification from target proteins (de-AMPylation), depending on the context. The side chain of Glu-231 determines which of the two opposing activities (AMPylase or de-AMPylase) will take place. Acts as a key regulator of the ERN1/IRE1-mediated unfolded protein response (UPR) by mediating AMPylation or de-AMPylation of HSPA5/BiP (PubMed:25601083). In unstressed cells, acts as an adenylyltransferase by mediating AMPylation of HSPA5/BiP at 'Thr-518', thereby inactivating it. In response to endoplasmic reticulum stress, acts as a phosphodiesterase by mediating removal of ATP (de-AMPylation) from HSPA5/BiP at 'Thr-518', leading to restore HSPA5/BiP activity. Although it is able to AMPylate RhoA, Rac and Cdc42 Rho GTPases in vitro, Rho GTPases do not constitute physiological substrates (PubMed:19362538, PubMed:25601083). [UniProt]
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: adenosine monophosphate-protein transferase FICD; AMPylator FICD; De-AMPylase FICD; FIC domain-containing protein; fic S-phase protein cell division homolog; HIP-13; huntingtin interacting protein 13; Huntingtin interacting protein E; huntingtin interactor protein E; Huntingtin yeast partner E; Huntingtin-interacting protein 13; Huntingtin-interacting protein E; MGC5623; Protein adenylyltransferase FICD
Gene Aliases: D5Ertd40e; FICD; HIP13; HYPE; UNQ3041; UNQ3041/PRO9857
UniProt ID: (Human) Q9BVA6, (Mouse) Q8BIX9, (Rat) Q6AY47
Entrez Gene ID: (Human) 11153, (Mouse) 231630, (Rat) 288741
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