Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Invitrogen
Hemoglobin Chimeric Recombinant Rabbit Monoclonal Antibody (2A1)
FIGURE: 1 / 1
Hemoglobin Chimeric Antibody (MA5-48118) in ICC/IF
Immunocytochemistry/Immunofluorescence analysis of Hemoglobin in mouse splenocytes using Hemoglobin Chimeric Monoclonal Antibody (Product # MA5-48118). Paraformaldehyde-fixed cells were immobilized on cover slips and stained with Hemoglobin Chimeric Monoclonal Antibody at 10 µg/mL for 1h followed by Alexa Fluor 488 secondary antibody (2 µg/mL), showing membrane staining. The nuclear stain is DAPI (blue). Pa... View More
Product Details
MA5-48118
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Rabbit
/ IgG, kappa
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
2A1
Immunogen
Murine hybridomas were generated using spleen cells from aging (8-10 month old) NZB/W F1 animals.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
PBS
Contains
0.02% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_3074561
Product Specific Information
Specificity: 2A1 exhibited polyreactive recognition of peptide 100-119 from the alpha subunit and peptide 100-119 frmo the beta subunit. Moreover, 2A1 bound Hb and the Hb-haptoglobin complex with equal efficiency, implying different binding sites on Hb for haptoglobin and 2A1. 2A1 was also found to distinguish between Hb and metHb.
Preparation: Murine hybridomas were generated using spleen cells from aging (8-10 month old) NZB/W F1 animals. Hybridomas secreting antibodies reactive to murine hemoglobin were subcloned by limiting dilution and antibody isotypes determined using commercial reagents. Eight-week-old NZB/W F1 were immunized subcutaneously with 50 mg mHb antigen emulsified in Complete Fruends Adjuvant.
Target Information
The human alpha globin gene cluster located on chromosome 16 spans about 30 kb and includes seven loci: 5'- zeta - pseudozeta - mu - pseudoalpha-1 - alpha-2 - alpha-1 - theta - 3'. The alpha-2 (HBA2) and alpha-1 (HBA1) coding sequences are identical. These genes differ slightly over the 5' untranslated regions and the introns, but they differ significantly over the 3' untranslated regions. Two alpha chains plus two beta chains constitute HbA, which in normal adult life comprises about 97% of the total hemoglobin; alpha chains combine with delta chains to constitute HbA-2, which with HbF (fetal hemoglobin) makes up the remaining 3% of adult hemoglobin. Alpha thalassemias result from deletions of each of the alpha genes as well as deletions of both HBA2 and HBA1; some nondeletion alpha thalassemias have also been reported.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: alpha-1 globin; alpha-1-globin; ALPHA-GLOBIN GENE; Haemoglobin; Hb; HbA1c
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support