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Product Details
MUB0801P
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bovine,
Fish,
Human,
Mouse,
Rat
Host/Isotype
Rat
/ IgG2a
Class
Monoclonal
Type
Antibody
Clone
A7L6
Immunogen
High molecular mass material derived from the Engelbreth-Holm-Swarm (EHS) tumor matrix containing laminin, entactin and HSPG
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A/G
Storage buffer
PBS
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
A7L6 recognizes domain IV of the core protein of the large heparan sulphate proteoglycan or perlecan. The reactivity is independent of the galactosaminoglycan moieties. Therefore, the epitope is not sensitive to heparitinase treatment.
The antibody may be stored at 4°C. For prolonged storage prepare appropriate aliquots and store at or below -20°C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at 4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance or the concentration of the product.
Target Information
Perlecan is a major heparan-sulfate proteoglycan (HSPG) found within all basement membranes and cell surfaces. Because of its strategic location and ability to store and protect growth factors, perlecan has been strongly implicated in the control of tumor cell growth and metastatic behavior. Perlecan possesses angiogenic and growth-promoting attributes primarily by acting as a coreceptor for basic fibroblast growth factor (FGF-2). Suppression of perlecan causes substantial inhibition of neoplastic growth and neovascularization. Thus, perlecan is a potent inducer of neoplasm growth and angiogenesis in vivo and therapeutic interventions targeting this key modulator of tumor progression may improve neoplastic treatment.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Basement membrane-specific heparan sulfate proteoglycan core protein; endorepellin (domain V region); HSPG; Perlecan; perlecan proteoglycan; PLC; RP11-132G19.2
Gene Aliases: AI852380; BOS_2497; HSPG; HSPG2; Pcn; per; PLC; PRCAN; SJA; SJS; SJS1
UniProt ID: (Human) P98160, (Mouse) Q05793
Entrez Gene ID: (Human) 3339, (Bovine) 444872, (Mouse) 15530, (Rat) 313641
retinoid metabolic process
angiogenesis
glycosaminoglycan biosynthetic process
glycosaminoglycan catabolic process
extracellular matrix disassembly
extracellular matrix organization
glycosaminoglycan metabolic process
lipoprotein metabolic process
cellular protein metabolic process
endochondral ossification
chondrocyte differentiation
brain development
protein localization
embryonic skeletal system morphogenesis
cardiac muscle tissue development
cartilage development involved in endochondral bone morphogenesis
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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