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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • 6x His Tag Antibodies

          Proteintech

          His-Tag Monoclonal Antibody (1B7G5)

          748 References
          View all (129) 6x His Tag antibodies

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          Datasheet
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          Cite His-Tag Monoclonal Antibody (1B7G5)

          • Antibody Testing Data (3)
          His-Tag Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.
          His-Tag Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 3

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          His-Tag Antibody (66005-1-IG) in ICC/IF

          Immunofluorescent analysis of ( -20°C Ethanol) fixed Transfected HEK-293 cells using 66005-1-IG (6xHis, His-Tag antibody) at dilution of 1:400 and Alexa Fluor 594-Conjugated AffiniPure Goat Anti-Mouse IGG (H+L). {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          View Product

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          His-Tag Antibody in Immunocytochemistry (ICC/IF)
          His-Tag Antibody in Western Blot (WB)
          His-Tag Antibody in Immunoprecipitation (IP)

          Product Details

          66005-1-IG

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:5,000-1:50,000
          View 387 publications 387 publications

          Immunohistochemistry (IHC)

          -
          View 3 publications 3 publications

          Immunocytochemistry (ICC/IF)

          1:200-1:800
          View 28 publications 28 publications

          Flow Cytometry (Flow)

          Assay-dependent
          View 2 publications 2 publications

          ELISA (ELISA)

          -
          View 2 publications 2 publications

          Immunoprecipitation (IP)

          0.5-4.0 µg
          View 67 publications 67 publications

          ChIP assay (ChIP)

          -
          View 1 publication 1 publication

          Miscellaneous PubMed (MISC)

          -
          View 65 publications 65 publications

          Not applicable (N/A)

          -
          View 193 publications 193 publications
          Product Specifications

          Species Reactivity

          Tag

          Published species

          Tag

          Host/Isotype

          Mouse / IgG1

          Class

          Monoclonal

          Type

          Antibody

          Clone

          1B7G5

          Immunogen

          Peptide

          Conjugate

          Unconjugated Unconjugated Unconjugated
          • Biotin 
          • CoraLite 594
          • CoraLite Plus 488
          • CoraLite Plus 647
          • CoraLite Plus 670
          • HRP
          • Request custom conjugation

          Form

          Liquid

          Concentration

          1.34 mg/mL

          Purification

          Protein G

          Storage buffer

          PBS, pH 7.3, with 50% glycerol

          Contains

          0.02% sodium azide

          Storage conditions

          -20°C

          Shipping conditions

          Wet ice (domestic); Dry ice (international)

          Product Specific Information

          The His-tag antibody is a useful tool for monitoring His-tagged proteins, and recognizes His-tags placed at N-terminal, C-terminal, and internal regions of fusion proteins expressed in bacteria, insect, and mammalian cells.

          Target Information

          The 6x His tag is a synthetic oligo peptide consisting of 6 consecutive histidine residues (HHHHHH). The His tag is commonly expressed as a tag at either N-or C-terminal regions of recombinant proteins to allow isolation or purification by immobilized metal affinity chromatography. Epitope tagging is a technique in which a known epitope is fused to a recombinant protein using genetic engineering. By choosing a particular epitope and recombinant protein combination, epitope tagging makes it possible to detect proteins for which no antibody is available. His epitope tagged proteins that contain a stretch of histidine residues at the carboxyl or amino terminus are used for purification of recombinant proteins by means of metal chelate chromatography due to metal binding properties of histidine clusters on His tagged proteins. For example, the affinity of the histidine-tag motif to Ni2+ by chelation is strong and selective enough to enable purification of the protein to homogeneity by affinity chromatography on a Ni2+-NTA adsorbent. His tag-specific antibodies are used to facilitate detection or coimmunopreciptation of tagged proteins.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          It has to be done as per old AB suggested Products section.
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          Performance Guarantee

          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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