GenScript

His Tag Monoclonal Antibody (6G2A9), iFluor™ 488, THE™

His Tag Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 2

His Tag Antibody (A01800-100) in ICC/IF

Immunocytochemistry/Immunofluorescence analysis of non-transfected CHO cells (a) or His-tagged protein transfected CHO cells (b). using THE™ His Tag Antibody [iFluor 488], mAb, Mouse (A01800-100, 4 µg/mL).

His Tag Antibody in Flow Cytometry (Flow)

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Product Details

A01800-100

Applications

Tested Dilution

Publications

Immunocytochemistry (ICC/IF)

1-4 µg/mL

Flow Cytometry (Flow)

1-4 µg/mL

Miscellaneous PubMed (MISC)

View 3 publications 3 publications

Product Specifications

Species Reactivity

Tag

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

6G2A9

Immunogen

A synthetic peptide HHHHHH coupled to KLH

Conjugate

iFluor™ 488 iFluor™ 488 iFluor™ 488

Excitation/Emission Max

491/516 nm

Form

Lyophilized

Purification

Protein A

Storage buffer

PBS, pH 7.4, with 10mg/mL BSA

Contains

0.02% sodium azide

Storage conditions

-20°C or -80°C if preferred

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Reconstitute the lyophilized product with deionized water (or equivalent) to make the antibody concentration of 0.5 mg/ml.

The His tag is a short amino acid sequence consisting of Histidine (His) residues appended to recombinant proteins. Ni-charged resins are used for the purification of His-tagged proteins from eukaryotic and prokaryotic expression systems. An anti-His tag antibody is a useful tool for the analysis of His-tagged proteins.THE™ His Tag Antibody [iFluor 488], mAb, Mouse is THE™ His Tag Antibody (A00186) conjugated with iFluor 488 under optimal conditions with an F/P ratio of 4-8. It is suitable for detecting the expression level of His-tagged proteins. iFluor 488 is a bright and photostable fluorescent dye. It is an excellent alternative to Alexa Fluor 488.

Target Information

The 6x His tag is a synthetic oligo peptide consisting of 6 consecutive histidine residues (HHHHHH). The His tag is commonly expressed as a tag at either N-or C-terminal regions of recombinant proteins to allow isolation or purification by immobilized metal affinity chromatography. Epitope tagging is a technique in which a known epitope is fused to a recombinant protein using genetic engineering. By choosing a particular epitope and recombinant protein combination, epitope tagging makes it possible to detect proteins for which no antibody is available. His epitope tagged proteins that contain a stretch of histidine residues at the carboxyl or amino terminus are used for purification of recombinant proteins by means of metal chelate chromatography due to metal binding properties of histidine clusters on His tagged proteins. For example, the affinity of the histidine-tag motif to Ni2+ by chelation is strong and selective enough to enable purification of the protein to homogeneity by affinity chromatography on a Ni2+-NTA adsorbent. His tag-specific antibodies are used to facilitate detection or coimmunopreciptation of tagged proteins.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Family of neural wiring receptors in bilaterians defined by phylogenetic, biochemical, and structural evidence.

Proceedings of the National Academy of Sciences of the United States of America

Cheng S,Park Y,Kurleto JD,Jeon M,Zinn K,Thornton JW,Özkan E

The evolution of complex nervous systems was accompanied by the expansion of numerous protein families, including cell-adhesion molecules, surface receptors, and their ligands. These proteins mediate axonal guidance, synapse targeting, and other neuronal wiring-related functions. Recently, 32 interacting cell surface proteins belonging to two newly defined families of the Ig superfamily (IgSF) in fruit flies were discovered to label different subsets of neurons in the brain and ventral nerve cor

Tue May 14 00:00:00 UTC 2019

Molecular basis of synaptic specificity by immunoglobulin superfamily receptors in Drosophila.

eLife

Cheng S,Ashley J,Kurleto JD,Lobb-Rabe M,Park YJ,Carrillo RA,Özkan E

In stereotyped neuronal networks, synaptic connectivity is dictated by cell surface proteins, which assign unique identities to neurons, and physically mediate axon guidance and synapse targeting. We recently identified two groups of immunoglobulin superfamily proteins in Drosophila, Dprs and DIPs, as strong candidates for synapse targeting functions. Here, we uncover the molecular basis of specificity in Dpr-DIP mediated cellular adhesions and neuronal connectivity. First, we present five cryst

Mon Jan 28 00:00:00 UTC 2019

Disruption of FOXP3-EZH2 Interaction Represents a Pathobiological Mechanism in Intestinal Inflammation.

Cellular and molecular gastroenterology and hepatology

Bamidele AO,Svingen PA,Sagstetter MR,Sarmento OF,Gonzalez M,Braga Neto MB,Kugathasan S,Lomberk G,Urrutia RA,Faubion WA

Forkhead box protein 3 (FOXP3) regulatory T cell (Treg) dysfunction is associated with autoimmune diseases; however the mechanisms responsible for inflammatory bowel disease pathophysiology are poorly understood. Here we tested the hypothesis that a physical interaction between transcription factor FOXP3 and the epigenetic enzyme enhancer of zeste homolog 2 (EZH2) is essential for gene co-repressive function.

Mon Apr 29 00:00:00 UTC 2019

Bioinformatics

Protein Aliases: 6His tag; 6X His; His Tag; Histidine Tag

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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