Proteintech

His tag Polyclonal Antibody

FIGURE: 1 / 1

His tag Antibody (10001-0-AP) in WB

Western blot of 6xHis-tagged fusion protein with anti-6xHIS tag (10001-0-AP) at various dilutions.

Product Image Gallery

Product Details

10001-0-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 35 publications 35 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

View 4 publications 4 publications

Immunoprecipitation (IP)

View 4 publications 4 publications

Miscellaneous PubMed (MISC)

View 3 publications 3 publications

Not applicable (N/A)

View 7 publications 7 publications

Product Specifications

Species Reactivity

Tag

Published species

Tag

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.4 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

The antibody reacts specifically with the 6xHis tag fused to recombinant protein.

Target Information

The 6x His tag is a synthetic oligo peptide consisting of 6 consecutive histidine residues (HHHHHH). The His tag is commonly expressed as a tag at either N-or C-terminal regions of recombinant proteins to allow isolation or purification by immobilized metal affinity chromatography. Epitope tagging is a technique in which a known epitope is fused to a recombinant protein using genetic engineering. By choosing a particular epitope and recombinant protein combination, epitope tagging makes it possible to detect proteins for which no antibody is available. His epitope tagged proteins that contain a stretch of histidine residues at the carboxyl or amino terminus are used for purification of recombinant proteins by means of metal chelate chromatography due to metal binding properties of histidine clusters on His tagged proteins. For example, the affinity of the histidine-tag motif to Ni2+ by chelation is strong and selective enough to enable purification of the protein to homogeneity by affinity chromatography on a Ni2+-NTA adsorbent. His tag-specific antibodies are used to facilitate detection or coimmunopreciptation of tagged proteins.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

USP33 enhances cell survival and stemness by deubiquitinating CTNNB1 in BXPC-3 and SW1990 cells.

Cell biology international

Zhong B,Zhu Q,Wang L,Wang F,Zheng Y,Lin S,Cheng X

Tue Aug 01 00:00:00 UTC 2023

A red light-controlled probiotic bio-system for in-situ gut-brain axis regulation.

Biomaterials

Zhang X,Pang G,Sun T,Liu X,Pan H,Zhang Y,Liu J,Chang J,Wang H,Liu D

Wed Mar 01 00:00:00 UTC 2023

Epithelial cells activate fibroblasts to promote esophageal cancer development.

Cancer cell

Chen Y,Zhu S,Liu T,Zhang S,Lu J,Fan W,Lin L,Xiang T,Yang J,Zhao X,Xi Y,Ma Y,Cheng G,Lin D,Wu C

Mon May 08 00:00:00 UTC 2023

Glioma-derived LRIG3 interacts with NETO2 in tumor-associated macrophages to modulate microenvironment and suppress tumor growth.

Cell death & disease

Li Y,Wang W,Hou X,Huang W,Zhang P,He Y,Wang B,Duan Q,Mao F,Guo D

Fri Jan 13 00:00:00 UTC 2023

Berberine promotes IGF2BP3 ubiquitination by TRIM21 to induce G1/S phase arrest in colorectal cancer cells.

Chemico-biological interactions

Gui Z,Li J,Li J,Li X,Chen L,Ma Z,Tang X,Gong X,Chai X,Lu F,Li M,Ma H,Li X,Ye X

Sat Apr 01 00:00:00 UTC 2023

Berberine promotes IGF2BP3 ubiquitination by TRIM21 to induce G1/S phase arrest in colorectal cancer cells.

Chemico-biological interactions

Gui Z,Li J,Li J,Li X,Chen L,Ma Z,Tang X,Gong X,Chai X,Lu F,Li M,Ma H,Li X,Ye X

Sat Apr 01 00:00:00 UTC 2023

MEX3A Mediates p53 Degradation to Suppress Ferroptosis and Facilitate Ovarian Cancer Tumorigenesis.

Cancer research

Wang CK,Chen TJ,Tan GYT,Chang FP,Sridharan S,Yu CA,Chang YH,Chen YJ,Cheng LT,Hwang-Verslues WW

Wed Jan 18 00:00:00 UTC 2023

MEX3A Mediates p53 Degradation to Suppress Ferroptosis and Facilitate Ovarian Cancer Tumorigenesis.

Cancer research

Wang CK,Chen TJ,Tan GYT,Chang FP,Sridharan S,Yu CA,Chang YH,Chen YJ,Cheng LT,Hwang-Verslues WW

Wed Jan 18 00:00:00 UTC 2023

KCNF1 promotes lung cancer by modulating ITGB4 expression.

Cancer gene therapy

Chen CY,Wu PY,Van Scoyk M,Simko SA,Chou CF,Winn RA

Wed Mar 01 00:00:00 UTC 2023

A Potential Adhesin/Invasin STM0306 Participates in Host Cell Inflammation Induced by Salmonella enterica Serovar Typhimurium.

International journal of molecular sciences

Ling C,Liang S,Li Y,Cao Q,Ye H,Zhang C,Dong Z,Feng D,Wang W,Zuo J

Wed May 03 00:00:00 UTC 2023

A peptide encoded by lncRNA MIR7-3 host gene (MIR7-3HG) alleviates dexamethasone-induced dysfunction in pancreatic β-cells through the PI3K/AKT signaling pathway.

Biochemical and biophysical research communications

Mao X,Zhou J,Kong L,Zhu L,Yang D,Zhang Z

Sun Mar 05 00:00:00 UTC 2023

Hepatocyte-derived MASP1-enriched small extracellular vesicles activate HSCs to promote liver fibrosis.

Hepatology (Baltimore, Md.)

Liu X,Tan S,Liu H,Jiang J,Wang X,Li L,Wu B

Sat Apr 01 00:00:00 UTC 2023

Hepatocyte-derived MASP1-enriched small extracellular vesicles activate HSCs to promote liver fibrosis.

Hepatology (Baltimore, Md.)

Liu X,Tan S,Liu H,Jiang J,Wang X,Li L,Wu B

Sat Apr 01 00:00:00 UTC 2023

A novel matrix protein PNU5 facilitates the transformation from amorphous calcium carbonate to calcite and aragonite.

International journal of biological macromolecules

Shuai B,Deng T,Xie L,Zhang R

Sun Jan 01 00:00:00 UTC 2023

Deletion of lacD gene affected stress tolerance and virulence of Streptococcus suis serotype 2.

Journal of microbiology (Seoul, Korea)

Jiang X,Zhu L,Zhan D

Thu Sep 01 00:00:00 UTC 2022

Type VI secretion system-associated FHA domain protein TagH regulates the hemolytic activity and virulence of Vibrio cholerae.

Gut microbes

Wang G,Fan C,Wang H,Jia C,Li X,Yang J,Zhang T,Gao S,Min X,Huang J

Thu Apr 07 00:00:00 UTC 2022

Programmable probiotics modulate inflammation and gut microbiota for inflammatory bowel disease treatment after effective oral delivery.

Nature communications

Zhou J,Li M,Chen Q,Li X,Chen L,Dong Z,Zhu W,Yang Y,Liu Z,Chen Q

Tue Jun 14 00:00:00 UTC 2022

A mutated glycosaminoglycan-binding domain functions as a novel probe to selectively target heparin-like epitopes on tumor cells.

The Journal of biological chemistry

Xu Y,Shi L,Qin Y,Yuan X,Wang X,Zhang Q,Wei L,Du M,Liu Y,Yuan M,Xu X,Cheng R,Zou R,Wang W,Li F

Thu Dec 01 00:00:00 UTC 2022

Oligomerization of Ca²⁺/calmodulin-dependent protein kinase kinase.

Biochemical and biophysical research communications

Fukumoto Y,Harada Y,Ohtsuka S,Kanayama N,Magari M,Hatano N,Sakagami H,Tokumitsu H

Sat Jan 08 00:00:00 UTC 2022

Flow cytometry-based multiplexing antibody detection for diagnosis of African swine fever virus.

Analytica chimica acta

Li C,Zou Z,Lv C,Zhao Y,Han P,Sun X,Jin M

Thu Sep 08 00:00:00 UTC 2022

GNIP1 functions both as a scaffold protein and an E3 ubiquitin ligase to regulate autophagy in lung cancer.

Cell communication and signaling : CCS

Zhou F,Liu Y,Ai W,Wang Y,Gan M,Jiang Q,Han T,Wang JB

Tue Aug 30 00:00:00 UTC 2022

ARNO is recruited by the neuronal adaptor FE65 to potentiate ARF6-mediated neurite outgrowth.

Open biology

Zhai Y,Chan WWR,Li W,Lau KF

Thu Sep 01 00:00:00 UTC 2022

UHRF1 Induces Metastasis in Thyroid Cancer.

Journal of oncology

Kuang BH,Lin GH,Liu Q,Wang BC

Wed Aug 24 00:00:00 UTC 2022

UHRF1 Induces Metastasis in Thyroid Cancer.

Journal of oncology

Kuang BH,Lin GH,Liu Q,Wang BC

Wed Aug 24 00:00:00 UTC 2022

UHRF1 Induces Metastasis in Thyroid Cancer.

Journal of oncology

Kuang BH,Lin GH,Liu Q,Wang BC

Wed Aug 24 00:00:00 UTC 2022

Targeting Procalcitonin Protects Vascular Barrier Integrity.

American journal of respiratory and critical care medicine

Brabenec L,Müller M,Hellenthal KEM,Karsten OS,Pryvalov H,Otto M,Holthenrich A,Matos ALL,Weiss R,Kintrup S,Hessler M,Dell'Aquila A,Thomas K,Naß J,Margraf A,Nottebaum AF,Rossaint J,Zarbock A,Vestweber D,Gerke V,Wagner NM

Mon Aug 15 00:00:00 UTC 2022

Identification of CD73 as the Antigen of an Antigen-Unknown Monoclonal Antibody Established by Exosome Immunization, and Its Antibody-Drug Conjugate Exerts an Antitumor Effect on Glioblastoma Cell Lines.

Pharmaceuticals (Basel, Switzerland)

Anzai T,Saijou S,Takashima H,Hara M,Hanaoka S,Matsumura Y,Yasunaga M

Wed Jul 06 00:00:00 UTC 2022

Grass Carp Prx 3 Elevates Host Antioxidant Activity and Induces Autophagy to Inhibit Grass Carp Reovirus (GCRV) Replication.

Antioxidants (Basel, Switzerland)

Liang X,Li Y,Chu P,Wang Q,Wang H,Liao L,Yang C,Zhu Z,Wang Y,He L

Thu Sep 29 00:00:00 UTC 2022

Phytochrome B enhances seed germination tolerance to high temperature by reducing S-nitrosylation of HFR1.

EMBO reports

Ying S,Yang W,Li P,Hu Y,Lu S,Zhou Y,Huang J,Hancock JT,Hu X

Thu Oct 06 00:00:00 UTC 2022

Pseudomonas aeruginosa PcrV Enhances the Nitric Oxide-Mediated Tumoricidal Activity of Tumor-Associated Macrophages via a TLR4/PI3K/AKT/mTOR-Glycolysis-Nitric Oxide Circuit.

Frontiers in oncology

Yu H,Bai Y,Qiu J,He X,Xiong J,Dai Q,Wang X,Li Y,Sheng H,Xin R,Jiang L,Li Q,Li D,Zhang H,Zhang L,Chen Q,Peng J,Hu X,Zhang K

Wed Dec 15 00:00:00 UTC 2021

TRIM25 regulates oxaliplatin resistance in colorectal cancer by promoting EZH2 stability.

Cell death & disease

Zhou S,Peng J,Xiao L,Zhou C,Fang Y,Ou Q,Qin J,Liu M,Pan Z,Hou Z

Sat May 08 00:00:00 UTC 2021

Structure of the multiple functional domains from coronavirus nonstructural protein 3.

Emerging microbes & infections

Li M,Ye G,Si Y,Shen Z,Liu Z,Shi Y,Xiao S,Fu ZF,Peng G

Wed Dec 01 00:00:00 UTC 2021

The acetylation modification regulates the stability of Bm30K-15 protein and its mechanism in silkworm, Bombyx mori.

Archives of insect biochemistry and physiology

Lv J,Li S,Liu Y,Sun Z,Wang D,You Z,Jiang C,Sheng Q,Nie Z

Thu Jul 01 00:00:00 UTC 2021

KAT2A-mediated AR translocation into nucleus promotes abiraterone-resistance in castration-resistant prostate cancer.

Cell death & disease

Lu D,Song Y,Yu Y,Wang D,Liu B,Chen L,Li X,Li Y,Cheng L,Lv F,Zhang P,Xing Y

Thu Aug 12 00:00:00 UTC 2021

Single-reaction multi-antigen serological test for comprehensive evaluation of SARS-CoV-2 patients by flow cytometry.

European journal of immunology

Cáceres-Martell Y,Fernández-Soto D,Campos-Silva C,García-Cuesta EM,Casasnovas JM,Navas-Herrera D,Beneítez-Martínez A,Martínez-Fleta P,Alfranca A,Sánchez-Madrid F,Escudero-López G,Vilches C,Jara-Acevedo R,Reyburn HT,Rodríguez-Frade JM,Valés-Gómez M

Mon Nov 01 00:00:00 UTC 2021

The stability and antiapoptotic activity of Bm30K-3 can be improved by lysine acetylation in the silkworm, Bombyx mori.

Archives of insect biochemistry and physiology

Ma Y,Wu C,Liu J,Liu Y,Lv J,Sun Z,Wang D,Jiang C,Sheng Q,You Z,Nie Z

Wed Apr 01 00:00:00 UTC 2020

Fibroblast Activation Protein (FAP) Overexpression Induces Epithelial-Mesenchymal Transition (EMT) in Oral Squamous Cell Carcinoma by Down-Regulating Dipeptidyl Peptidase 9 (DPP9).

OncoTargets and therapy

Wu QQ,Zhao M,Huang GZ,Zheng ZN,Chen Y,Zeng WS,Lv XZ

Wed Apr 13 00:00:00 UTC 2022

Identification of a novel host protein interacting with the structural protein VP2 of deformed wing virus by yeast two-hybrid screening.

Virus research

Huang S,Fei D,Ma Y,Wang C,Shi D,Liu K,Li M,Ma M

Tue Sep 01 00:00:00 UTC 2020

Preparation, identification, and functional analysis of monoclonal antibodies against atypical porcine pestivirus NS3 protein.

Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc

Yan M,Huang J,Chen J,Yang W,Liu G

Tue Sep 01 00:00:00 UTC 2020

Codon optimization, expression in Escherichia coli, and immunogenicity analysis of deformed wing virus (DWV) structural protein.

PeerJ

Fei D,Guo Y,Fan Q,Li M,Sun L,Ma M,Li Y

Wed Apr 13 00:00:00 UTC 2022

A missense in HSF2BP causing primary ovarian insufficiency affects meiotic recombination by its novel interactor C19ORF57/BRME1.

eLife

Felipe-Medina N,Caburet S,Sánchez-Sáez F,Condezo YB,de Rooij DG,Gómez-H L,Garcia-Valiente R,Todeschini AL,Duque P,Sánchez-Martin MA,Shalev SA,Llano E,Veitia RA,Pendás AM

Wed Aug 26 00:00:00 UTC 2020

Salinomycin exerts anti-colorectal cancer activity by targeting the β-catenin/T-cell factor complex.

British journal of pharmacology

Wang Z,Zhou L,Xiong Y,Yu S,Li H,Fan J,Li F,Su Z,Song J,Sun Q,Liu SS,Xia Y,Zhao L,Li S,Guo F,Huang P,Carson DA,Lu D

Sun Sep 01 00:00:00 UTC 2019

Functionally critical residues in the aminoglycoside resistance-associated methyltransferase RmtC play distinct roles in 30S substrate recognition.

The Journal of biological chemistry

Nosrati M,Dey D,Mehrani A,Strassler SE,Zelinskaya N,Hoffer ED,Stagg SM,Dunham CM,Conn GL

Fri Nov 15 00:00:00 UTC 2019

FRUCTOKINASE-LIKE PROTEIN 1 interacts with TRXz to regulate chloroplast development in rice.

Journal of integrative plant biology

He L,Zhang S,Qiu Z,Zhao J,Nie W,Lin H,Zhu Z,Zeng D,Qian Q,Zhu L

Thu Feb 01 00:00:00 UTC 2018

FRUCTOKINASE-LIKE PROTEIN 1 interacts with TRXz to regulate chloroplast development in rice.

Journal of integrative plant biology

He L,Zhang S,Qiu Z,Zhao J,Nie W,Lin H,Zhu Z,Zeng D,Qian Q,Zhu L

Thu Feb 01 00:00:00 UTC 2018

USP52 acts as a deubiquitinase and promotes histone chaperone ASF1A stabilization.

Nature communications

Yang S,Liu L,Cao C,Song N,Wang Y,Ma S,Zhang Q,Yu N,Ding X,Yang F,Tian S,Zhang K,Sun T,Yang J,Yao Z,Wu S,Shi L

Thu Mar 29 00:00:00 UTC 2018

USP52 acts as a deubiquitinase and promotes histone chaperone ASF1A stabilization.

Nature communications

Yang S,Liu L,Cao C,Song N,Wang Y,Ma S,Zhang Q,Yu N,Ding X,Yang F,Tian S,Zhang K,Sun T,Yang J,Yao Z,Wu S,Shi L

Thu Mar 29 00:00:00 UTC 2018

Regulation of energy homeostasis by the ubiquitin-independent REGγ proteasome.

Nature communications

Sun L,Fan G,Shan P,Qiu X,Dong S,Liao L,Yu C,Wang T,Gu X,Li Q,Song X,Cao L,Li X,Cui Y,Zhang S,Wang C

Thu Aug 11 00:00:00 UTC 2016

Regulation of energy homeostasis by the ubiquitin-independent REGγ proteasome.

Nature communications

Sun L,Fan G,Shan P,Qiu X,Dong S,Liao L,Yu C,Wang T,Gu X,Li Q,Song X,Cao L,Li X,Cui Y,Zhang S,Wang C

Thu Aug 11 00:00:00 UTC 2016

MNRR1 (formerly CHCHD2) is a bi-organellar regulator of mitochondrial metabolism.

Mitochondrion

Aras S,Bai M,Lee I,Springett R,Hüttemann M,Grossman LI

Thu Jan 01 00:00:00 UTC 2015

MNRR1 (formerly CHCHD2) is a bi-organellar regulator of mitochondrial metabolism.

Mitochondrion

Aras S,Bai M,Lee I,Springett R,Hüttemann M,Grossman LI

Thu Jan 01 00:00:00 UTC 2015

An important amino acid in nucleoprotein contributes to influenza A virus replication by interacting with polymerase PB2.

Virology

Gui X,Li R,Zhang X,Shen C,Yu H,Guo X,Kang Y,Chen J,Chen H,Chen Y,Xia N

Mon Sep 01 00:00:00 UTC 2014

An important amino acid in nucleoprotein contributes to influenza A virus replication by interacting with polymerase PB2.

Virology

Gui X,Li R,Zhang X,Shen C,Yu H,Guo X,Kang Y,Chen J,Chen H,Chen Y,Xia N

Mon Sep 01 00:00:00 UTC 2014

Regulation of the transcriptional activation of the androgen receptor by the UXT-binding protein VHL.

The Biochemical journal

Chen S,Chen K,Zhang Q,Cheng H,Zhou R

Fri Nov 15 00:00:00 UTC 2013

Regulation of the transcriptional activation of the androgen receptor by the UXT-binding protein VHL.

The Biochemical journal

Chen S,Chen K,Zhang Q,Cheng H,Zhou R

Fri Nov 15 00:00:00 UTC 2013

Silencing of OSBP-related protein 8 (ORP8) modifies the macrophage transcriptome, nucleoporin p62 distribution, and migration capacity.

Experimental cell research

Béaslas O,Vihervaara T,Li J,Laurila PP,Yan D,Olkkonen VM

Mon Sep 10 00:00:00 UTC 2012

Silencing of OSBP-related protein 8 (ORP8) modifies the macrophage transcriptome, nucleoporin p62 distribution, and migration capacity.

Experimental cell research

Béaslas O,Vihervaara T,Li J,Laurila PP,Yan D,Olkkonen VM

Mon Sep 10 00:00:00 UTC 2012

Crystal structure of Human ASB9-2 and substrate-recognition of CKB.

The protein journal

Fei X,Gu X,Fan S,Yang Z,Li F,Zhang C,Gong W,Mao Y,Ji C

Sun Apr 01 00:00:00 UTC 2012

Crystal structure of Human ASB9-2 and substrate-recognition of CKB.

The protein journal

Fei X,Gu X,Fan S,Yang Z,Li F,Zhang C,Gong W,Mao Y,Ji C

Sun Apr 01 00:00:00 UTC 2012

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

His tag Polyclonal Antibody

His tag Antibody (10001-0-AP) in WB

Product Details

10001-0-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Miscellaneous PubMed (MISC)

Not applicable (N/A)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Performance Guarantee

We're here to help

Your items have has been added!