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Invitrogen
His6-DEP-1 Polyclonal Antibody
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His6-DEP-1 Antibody (PA5-87692) in WB
Western blot analysis of His6-DEP-1 in extracts of Raji cells. Samples were incubated with His6-DEP-1 Polyclonal antibody (Product # PA5-87692) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Product Details
PA5-87692
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 140-370 of human PTPRJ (NP_0028343)
Conjugate
Form
Liquid
Concentration
1.42 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2804349
Product Specific Information
Immunogen sequence: KSNDTAASEY KYVVKHKMEN EKTITVVHQP WCNITGLRPA TSYVFSITPG IGNETWGDPR VIKVITEPIP VSDLRVALTG VRKAALSWSN GNGTASCRVL LESIGSHEEL TQDSRLQVNI SGLKPGVQYN INPYLLQSNK TKGDPLGTEG GLDASNTERS RAGSPTAPVH DESLVGPVDP SSGQQSRDTE VLLVGLEPGT RYNATVYSQA ANGTEGQPQA IEFRTNAIQV F; Positive Samples: Raji, SW480, Mouse spleen; Cellular Location: Cell junction, Cell membrane, Cell projection, Single-pass type I membrane protein, ruffle membrane
Target Information
The protein encoded by His6-DEP-1 is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes, including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP possesses an extracellular region containing five fibronectin type III repeats, a single transmembrane region, and a single intracytoplasmic catalytic domain, and thus represents a receptor-type PTP. This protein is present in all hematopoietic lineages, and was shown to negatively regulate T cell receptor signaling possibly through interfering with the phosphorylation of Phospholipase C Gamma 1 and Linker for Activation of T Cells. This protein can also dephosphorylate the PDGF beta receptor, and may be involved in UV-induced signal transduction. Multiple transcript variants encoding different isoforms have been found for His6-DEP-1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CD148; CD148 antigen; colon tumor susceptibility 1; Density-enhanced phosphatase 1; DEP-1; HPTP beta-like tyrosine phosphatase; HPTP eta; human density enhanced phosphatase-1; protein tyrosine phosphatase, receptor type, J polypeptide; Protein-tyrosine phosphatase eta; Protein-tyrosine phosphatase receptor type J; R-PTP-eta; R-PTP-J; Receptor-type tyrosine-protein phosphatase eta; Susceptibility to colon cancer 1; susceptibility to colon cancer 1, mouse, homolog of
Gene Aliases: AI450271; BET; Byp; CD148; DEP-1; DEP1; HPTPeta; Ptpb2; PTPbeta2; PTPRJ; R-PTP-ETA; RPTPJ; Scc-1; SCC1
UniProt ID: (Human) Q12913, (Mouse) Q64455
Entrez Gene ID: (Human) 5795, (Mouse) 19271
protein tyrosine phosphatase activity
platelet-derived growth factor receptor binding
protein binding
beta-catenin binding
phosphatase activity
protein kinase binding
gamma-catenin binding
mitogen-activated protein kinase binding
delta-catenin binding
phosphoprotein phosphatase activity
hydrolase activity
vasculogenesis
positive regulation of cell-matrix adhesion
protein dephosphorylation
heart development
blood coagulation
negative regulation of cell proliferation
positive regulation of platelet activation
negative regulation of platelet-derived growth factor receptor signaling pathway
positive regulation of macrophage chemotaxis
dephosphorylation
regulation of cell adhesion
negative regulation of cell growth
negative regulation of cell migration
peptidyl-tyrosine dephosphorylation
negative regulation of epidermal growth factor receptor signaling pathway
negative regulation of vascular permeability
negative regulation of MAP kinase activity
positive regulation of cell adhesion
platelet-derived growth factor receptor signaling pathway
oligodendrocyte differentiation
negative regulation of T cell receptor signaling pathway
positive chemotaxis
positive regulation of focal adhesion assembly
positive regulation of protein kinase B signaling
negative regulation of protein kinase B signaling
negative regulation of receptor activity
T cell receptor signaling pathway
contact inhibition
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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