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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • HisG Epitope Tag Antibodies

          Invitrogen

          HisG Epitope Tag Monoclonal Antibody, HRP

          2 Published Figures
          3 References
          View all (3) HisG Epitope Tag antibodies

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          Cite HisG Epitope Tag Monoclonal Antibody, HRP

          • Antibody Testing Data (1)
          • Published Figures (2)
          HisG Epitope Tag Antibody in Western Blot (WB)
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          HisG Epitope Tag Antibody in Western Blot (WB)
          Group 53 Created with Sketch.

          FIGURE: 1 / 3

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          HisG Epitope Tag Antibody (R941-25) in WB

          Western blot analysis of HisG was performed by loading 20 µg of whole cell extracts of untransfected HEK-293 cell lysate (lane1) and HEK-293 transiently over expressing HisG-RPS6 (lane2) using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0321BOX), Xcell SureLock Electrophoresis system (Product # EI0002), Novex sharp Pre-stained Protein Standard (LC5800), and iBlot® Dry Blotting System (IB21001). Proteins were transferred to a nitrocellulose membrane and blocked with 5 % skim milk for at least 1 hour at room temperature. HisG was detecte... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          HisG Epitope Tag Antibody in Western Blot (WB)
          HisG Epitope Tag Antibody in Western Blot (WB)
          HisG Epitope Tag Antibody in Western Blot (WB)

          Product Details

          R941-25

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:1,000
          View 3 publications 3 publications

          ELISA (ELISA)

          Assay-dependent
          -
          Product Specifications

          Species Reactivity

          Tag

          Published species

          Tag

          Host/Isotype

          Mouse / IgG2a

          Class

          Monoclonal

          Type

          Antibody

          Immunogen

          6x His synthetic peptide: -His-His-His-His-His-His-Gly

          Conjugate

          HRP HRP HRP

          Form

          Liquid

          Purification

          purified

          Storage buffer

          PBS

          Contains

          no preservative

          Storage conditions

          Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

          RRID

          AB_2556558

          Product Specific Information

          NOTE: R941-25 was previously sold under product #46-1009. When re-ordering, please use #R941-25.

          This antibody is designed to specifically detect recombinant proteins containing the seven amino acid 6xHis-Gly epitope. This epitope is present in many expression vectors encoding an N-terminal 6xHis tag. The HisG antibody recognizes the following sequence: -His-His-His-His-His-His-Gly and can be used to detect expression of fusion proteins from bacterial, insect, and mammalian cells.

          Anti-HisG-HRP Antibody was prepared by crosslinking the Anti-HisG Antibody with horseradish peroxidase using glutaraldehyde. It has been tested in immunoblotting and ELISA procedures. It was tested against purified Positope™ control protein (5 ng). The Positope™ control protein is a 53 kDa recombinant protein that contains seven epitope tags, including His(C-term), HisG, c-Myc, and V5. Low background was observed using chemiluminescent or alkaline phosphatase reagents for detection. In western blot experiments with purified protein, 50 ng (for Anti-HisG-HRP Antibody) of the Positope protein gave a detectable signal.

          For western blot, dilute in PBS containing 0.05% Tween-20 and 5% nonfat, dry milk (PBSTM). Using chemiluminescence as the detection method, no cross-reactivity has been observed in bacterial lysates. In mammalian lysates, a few cross-reactive proteins have been observed upon overexposure of blots. If you use alkaline phosphatase-conjugated secondary antibody, do not use PBS. Phosphate inhibits alkaline phosphatase. Use Tris-Buffered Saline (TBS) instead. Be sure to wash the western blot or microtiter wells before adding the horseradish peroxidase-conjugated secondary antibody. Azide will inhibit HRP activity. This product contains enough material for 25 western blots; assumes 10 mL buffer per western blot.

          Target Information

          Epitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. Expression of recombinant proteins in E. coli as a fusion protein with neighboring histidine residues is one of the most popular methods of epitope tags. The affinity of the histidine-tag motif to Ni2+ by chelation is strong and selective enough to enable purification of the protein to homogeneity by affinity chromatography on a Ni2+-NTA adsorbent. HisG antibodies allow detection of recombinant proteins containing a polyhistidine sequence: His-His-His-His-His-His-Gly (6xHis-Gly epitope). HisG antibodies can be used to detect expression of fusion proteins from bacterial, insect, and mammalian cells.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          It has to be done as per old AB suggested Products section.
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          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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