Invitrogen

Histone H2A.X Polyclonal Antibody

3 Published Figures

4 References

View all (73) Histone H2A.X antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (16)
Published Figures (3)

Histone H2A.X Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 19

Histone H2A.X Antibody (PA5-28778) in ICC/IF

Histone H2A. X Polyclonal Antibody detects Histone H2A. X protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Histone H2A. X stained by Histone H2A. X Polyclonal Antibody (Product # PA5-28778) diluted at 1:2,000. Red: phalloidin, a cytoskeleton marker, diluted at 1:200. Scale bar= 10 µm.

Histone H2A.X Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Histone H2A.X Antibody in Western Blot (WB)

Histone H2A.X Antibody in Immunoprecipitation (IP)

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Product Details

PA5-28778

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:20,000

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000

Immunocytochemistry (ICC/IF)

1:100-1:1,000

View 3 publications 3 publications

Immunoprecipitation (IP)

1:100-1:500

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Histone H2A.X. The exact sequence is proprietary.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.41 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 20% glycerol

Contains

0.025% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2546254

Product Specific Information

Recommended positive controls: 293T, HepG2, HCT116, HCT116 (30uM cisplatin for 24hr), Neuro 2A, C8D30, NIH-3T3, Raw264.7, C2C12, mouse brain, rat brain, PC-12, Rat2, 4T1.

Predicted reactivity: Rhesus Monkey (100%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

Histone H2A.X (H2AX) is a member of the histone H2A family which is one of the four core histones making up the nucleosome core particle. In eukaryotes, DNA double strand breaks (DSBs) have been shown to trigger the phosphorylation of serine 139 at the carboxy terminus of histone H2AX resulting in gamma-H2AX. The phosphorylation of H2AX can be detected by Western blotting or immunofluorescence, revealing the frequency of DSBs. The phosphatidylinositol 3-kinases have been implicated in H2AX phosphorylation, but it is unclear if ATM is the primary H2AX kinase or if other members of the family such as DNA-PK and ATR contribute in a similar manner. Structurally, H2A.x contains 143 amino acid residues. Histone H2A.X is considered a basal histone, being synthesized in G1 as well as in S-phase, and its mRNA contains polyA addition motifs and a polyA tail along with the conserved stem-loop and U7 binding sequences involved in the processing and stability of replication type histone mRNAs. There are two forms of Histone H2A.X mRNA, one about 1600 bases long and contains polyA; the other about 575 bases long, lacking polyA. The short form behaves as a replication type histone mRNA, while the longer behaves as a basal type histone mRNA. Histone H2A.X maps to the 11q23.2-q23.3 region of the human chromosome. Histone H2A.x contributes to histone-formation and therefore the structure of DNA. Histone H2A variant H2A.x specifically regulates the interaction of MDC1 (mediator of DNA damage checkpoint protein 1), a DNA repair protein to the sites of DNA damage.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Transglutaminase type 2-dependent crosslinking of IRF3 in dying melanoma cells.

Cell death discovery

Occhigrossi L,D'Eletto M,Vecchio A,Piacentini M,Rossin F

PA5-28778 was used in Immunocytochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot to demonstrate that TG2 negatively regulates the IRF3 pathway in human melanoma cells suggesting a so far unknown TG2-dependent mechanism by which cancer cells reduce the IFNI production after DNA damage to limit the immune system response.

Mon Dec 26 00:00:00 UTC 2022

The gut microbiota metabolite urolithin A inhibits NF-κB activation in LPS stimulated BMDMs.

Scientific reports

Abdelazeem KNM,Kalo MZ,Beer-Hammer S,Lang F

PA5-28778 was used in Immunocytochemistry-immunoflourescence to investigated the effect of urolithin A on the suppression of pro-inflammatory molecules and NF-κB activation by targeting TLR4 signalling pathway in BMDMs.

Mon Mar 29 00:00:00 UTC 2021

The gut microbiota metabolite urolithin A inhibits NF-κB activation in LPS stimulated BMDMs.

Scientific reports

Abdelazeem KNM,Kalo MZ,Beer-Hammer S,Lang F

Mon Mar 29 00:00:00 UTC 2021

Rapamycin-PLGA microparticles prevent senescence, sustain cartilage matrix production under stress and exhibit prolonged retention in mouse joints.

Biomaterials science

Dhanabalan KM,Gupta VK,Agarwal R

PA5-28778 was used in Immunocytochemistry to show that such particulate systems are promising candidates for intra-articular delivery of rapamycin for the treatment of osteoarthritis.

Fri Aug 07 00:00:00 UTC 2020

Chronic Intermittent Hypoxia Triggers a Senescence-like Phenotype in Human White Preadipocytes.

Scientific reports

Polonis K,Becari C,Chahal CAA,Zhang Y,Allen AM,Kellogg TA,Somers VK,Singh P

PA5-28778 was used in Immunocytochemistry-immunoflourescence to demonstrate that chronic intermittent hypoxia (IH) is associated with an increased generation of mitochondrial reactive oxygen species along with increased prevalence of cells with nuclear localization of γH2AX & p16.

Wed Apr 22 00:00:00 UTC 2020

Chronic Intermittent Hypoxia Triggers a Senescence-like Phenotype in Human White Preadipocytes.

Scientific reports

Polonis K,Becari C,Chahal CAA,Zhang Y,Allen AM,Kellogg TA,Somers VK,Singh P

Wed Apr 22 00:00:00 UTC 2020

Bioinformatics

Protein Aliases: gamma H2AX; H2A histone family, member X; H2a/x; H2AX histone; histone 5 protein 2ax; Histone H2A.X; Histone H2a/x; Histone H2AX

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Gene Aliases: AW228881; gammaH2ax; H2A.X; H2A/X; H2AFX; H2AX; Hist5-2ax; RGD1566119

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UniProt ID: (Human) P16104, (Mouse) P27661

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Entrez Gene ID: (Human) 3014, (Rat) 500987, (Mouse) 15270

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Function(s)

DNA binding damaged DNA binding protein binding enzyme binding histone binding protein heterodimerization activity molecular_function

Process(es)

DNA damage checkpoint double-strand break repair via homologous recombination double-strand break repair double-strand break repair via nonhomologous end joining nucleosome assembly chromatin silencing cellular response to DNA damage stimulus spermatogenesis response to ionizing radiation viral process cerebral cortex development positive regulation of DNA repair meiotic cell cycle cellular response to gamma radiation cellular senescence DNA repair DNA recombination cell cycle

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Histone H2A.X Polyclonal Antibody

Histone H2A.X Antibody (PA5-28778) in ICC/IF

Product Details

PA5-28778

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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