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Invitrogen
Histone H2B Polyclonal Antibody
FIGURE: 1 / 4
Histone H2B Antibody (PA1-41058) in WB
Western blot analysis was performed on acid extracts (20 µg lysate) of HeLa (Lane 1), K-562 (Lane 2), MCF7 (Lane 3), COS-7 (Lane 4) and NIH/3T3 (Lane 5). The blot was probed with Anti-Histone H2B (Product # PA1-41058, 2 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). A 17 kDa band corresponding to HistoneH2B was observed across the cell lines tested.
Product Details
PA1-41058
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Dog,
Chicken,
Fruit fly,
Human,
Mammal,
Mouse,
Rat,
Zebrafish
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide corresponding to amino acids 111-125 of human histone H2B [GenBank no. CAI19747.1]
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein G
Storage buffer
PBS
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2118162
Product Specific Information
This peptide sequence is 100% conserved between multiple species including human, mouse, rat, dog, chimpanzee, cow, chicken, duck, zebrafish, and drosophila.
Suggested positive control: Jurkat, PBMC.
Target Information
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The nucleosomes wrap further and compact DNA into chromotin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones play a central role in transciption regulation, DNA repair, DNA replication, and chromosomal stability. H2B has a broad antibacterial activity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Acetyl-Histone H2; CG17949-PA; CG33868-PA; CG33870-PA; CG33872-PA; CG33874-PA; CG33876-PA; CG33878-PA; CG33880-PA; CG33882-PA; CG33884-PA; CG33886-PA; CG33888-PA; CG33890-PA; CG33892-PA; CG33894-PA; CG33896-PA; CG33898-PA; CG33900-PA; CG33902-PA; CG33904-PA; CG33906-PA; CG33908-PA; CG33910-PA; H2B; H2B 291A; H2B 291B; H2b 613; H2B GL105 antibody; H2B histone family member W testis-specific; H2B histone family, member A; H2B histone family, member B; H2B histone family, member C; H2B histone family, member D; H2B histone family, member E; H2B histone family, member F; H2B histone family, member J; H2B histone family, member K; H2B histone family, member L; H2B histone family, member M; H2B histone family, member N; H2B histone family, member Q; H2B histone family, member R; H2B histone family, member S; H2B histone family, member T; H2B histone family, member U, (testis-specific); H2B histone family, member W, testis-specific; H2B K; H2B type 12; h2B-143; h2B-221; H2B-clustered histone 12 like; H2B-clustered histone 17; H2B-clustered histone 18; H2B-clustered histone 21; H2B-clustered histone 26; H2B-clustered histone 3; H2B-clustered histone 5; H2B-clustered histone 9; H2B.1 antibody; H2B.S histone 1; H2B.U histone 1; H2B.W histone 1; H2B/a; H2B/b; H2B/c; H2B/d; H2B/e; H2B/f; H2B/g; H2B/h; H2B/I; H2B/j; H2B/k; H2B/l; H2B/n; H2B/q; H2B/r; H2B/s; H2BK12ac; H2BK15ac; H2BK5ac; H2BS14; H2BS14p; HIRA-interacting protein 1; HIRA-interacting protein 2; His2B:CG17949-PA; His2B:CG33868-PA; His2B:CG33870-PA; His2B:CG33872-PA; His2B:CG33874-PA; His2B:CG33876-PA; His2B:CG33878-PA; His2B:CG33880-PA; His2B:CG33882-PA; His2B:CG33884-PA; His2B:CG33886-PA; His2B:CG33888-PA; His2B:CG33890-PA; His2B:CG33892-PA; His2B:CG33894-PA; His2B:CG33896-PA; His2B:CG33898-PA; His2B:CG33900-PA; His2B:CG33902-PA; His2B:CG33904-PA; His2B:CG33906-PA; His2B:CG33908-PA; His2B:CG33910-PA; HIST1H2BB antibody; HIST1H3I; histone 1, H2ba; histone 1, H2bb; histone 1, H2bc; histone 1, H2bd; histone 1, H2bg; histone 1, H2bh; histone 1, H2bi; histone 1, H2bj; histone 1, H2bk; histone 1, H2bl; histone 1, H2bm; histone 1, H2bn; histone 1, H2bo; histone 2, H2be; Histone 2B; histone 3, H2bb; histone cluster 1, H2ba; histone cluster 1, H2bc; histone cluster 1, H2bd; histone cluster 1, H2bm; histone cluster 1, H2bn; histone cluster 1, H2bo; histone cluster 3, H2bb; histone family member; Histone H2B; Histone H2B 1/2; Histone H2B 3; histone H2B type 1; Histone H2B type 1-A; Histone H2B type 1-B; Histone H2B type 1-C/E/F/G/I; Histone H2B type 1-C/E/G; Histone H2B type 1-D; Histone H2B type 1-F/J/L; Histone H2B type 1-H; Histone H2B type 1-J; Histone H2B type 1-K; Histone H2B type 1-L; Histone H2B type 1-M; Histone H2B type 1-N; Histone H2B type 1-O; Histone H2B type 2-E; Histone H2B type 2-F; Histone H2B type 3-B; Histone H2B type F-M; Histone H2B type F-S; histone H2B type F-S-like; Histone H2B type W-T; Histone H2B, testis; Histone H2B-GL105; Histone H2B.1; Histone H2B.1 A; Histone H2B.1 B; Histone H2B.2; Histone H2B.a; Histone H2B.b; Histone H2B.c; Histone H2B.d; Histone H2B.e; Histone H2B.f; Histone H2B.g; Histone H2B.h; Histone H2B.I; Histone H2B.j; Histone H2B.k; Histone H2B.l; Histone H2B.n; Histone H2B.q; Histone H2B.r; Histone H2B.s; MGC125414; MGC125415; MGC125416; MGC9388; RP1-193B12.10; testis-specific histone 2b; Testis-specific histone H2B; TSH2B.1
Gene Aliases: 2610022J01Rik; AV127319; bA317E16.3; CG17949; CG33868; CG33870; CG33872; CG33874; CG33876; CG33878; CG33880; CG33882; CG33884; CG33886; CG33888; CG33890; CG33892; CG33894; CG33896; CG33898; CG33900; CG33902; CG33904; CG33906; CG33908; CG33910; dJ160A22.3; dJ193B12.2; dJ221C16.3; dJ221C16.6; dJ221C16.8; dJ97D16.4; Dmel\CG17949; Dmel\CG33868; Dmel\CG33870; Dmel\CG33872; Dmel\CG33874; Dmel\CG33876; Dmel\CG33878; Dmel\CG33880; Dmel\CG33882; Dmel\CG33884; Dmel\CG33886; Dmel\CG33888; Dmel\CG33890; Dmel\CG33892; Dmel\CG33894; Dmel\CG33896; Dmel\CG33898; Dmel\CG33900; Dmel\CG33902; Dmel\CG33904; Dmel\CG33906; Dmel\CG33908; Dmel\CG33910; Dmel_CG17949; Dmel_CG33868; Dmel_CG33870; Dmel_CG33872; Dmel_CG33874; Dmel_CG33876; Dmel_CG33878; Dmel_CG33880; Dmel_CG33882; Dmel_CG33884; Dmel_CG33886; Dmel_CG33888; Dmel_CG33890; Dmel_CG33892; Dmel_CG33894; Dmel_CG33896; Dmel_CG33898; Dmel_CG33900; Dmel_CG33902; Dmel_CG33904; Dmel_CG33906; Dmel_CG33908; Dmel_CG33910; fj05g08; GL105; H2B; H2b-143; H2b-221; H2b-291b; H2b-613; H2b-f; H2b-j; H2b-l; H2b-n; H2B.1; H2B.1A; H2B.1B; H2B.2; H2B/a; H2B/b; H2B/c; H2B/d; H2B/e; H2B/f; H2B/j; H2B/k; H2B/l; H2B/n; H2B/r; H2B/S; H2Bb; H2BC1; H2BC10; H2BC11; H2BC12; H2BC12L; H2BC13; H2BC14; H2BC15; H2BC17; H2BC18; H2BC21; H2BC26; H2BC3; H2BC4; H2BC5; H2BC6; H2BC7; H2BC8; H2BC9; H2BFA; H2BFAiii; H2BFB; H2BFC; H2BFD; H2BFE; H2BFF; H2BFG; H2BFH; H2BFJ; H2BFK; H2BFL; H2BFM; H2BFN; H2BFQ; H2BFR; H2BFS; H2BFT; H2BFU; H2BFWT; H2BGL105; H2BJ; H2BK; H2BQ; H2BS1; H2BU1; H2BW1; H2BW2; HIRIP1; HIRIP2; His2B; His2B:CG17949; His2B:CG33868; His2B:CG33870; His2B:CG33872; His2B:CG33874; His2B:CG33876; His2B:CG33878; His2B:CG33880; His2B:CG33882; His2B:CG33884; His2B:CG33886; His2B:CG33888; His2B:CG33890; His2B:CG33892; His2B:CG33894; His2B:CG33896; His2B:CG33898; His2B:CG33900; His2B:CG33902; His2B:CG33904; His2B:CG33906; His2B:CG33908; His2B:CG33910; HIST1H2BA; HIST1H2BB; HIST1H2BC; HIST1H2BD; HIST1H2BE; HIST1H2BF; HIST1H2BG; HIST1H2BH; HIST1H2BI; HIST1H2BJ; HIST1H2BK; HIST1H2BL; HIST1H2BM; HIST1H2BN; HIST1H2BO; HIST2H2BE; HIST2H2BF; hist2h2l; HIST3H2BB; R74621; si:dkey-108k21.9; STBP; T25626; TH2B; TH2B-175; TSH2B; TSH2B.1; wu:fj05g08; zgc:112234; zgc:114046; zgc:73093
UniProt ID: (Human) P0C1H6, (Human) Q7Z2G1, (Human) Q96A08, (Human) P33778, (Human) P62807, (Human) Q93079, (Human) P06899, (Human) O60814, (Human) Q99880, (Human) Q99879, (Human) Q99877, (Human) P23527, (Human) Q16778, (Human) Q5QNW6, (Human) Q8N257, (Fruit fly) P02283, (Mouse) P70696, (Rat) Q00729, (Mouse) Q64475, (Mouse) Q6ZWY9, (Mouse) Q64478, (Mouse) P10853, (Mouse) Q8CGP1, (Mouse) P10854, (Mouse) Q64524, (Zebrafish) Q5BJA5, (Human) P57053, (Zebrafish) Q6PC60
Entrez Gene ID: (Human) 286436, (Human) 158983, (Dog) 491396, (Human) 255626, (Dog) 488252, (Human) 3018, (Human) 8347, (Human) 3017, (Human) 8339, (Human) 8345, (Human) 8346, (Human) 8970, (Human) 85236, (Human) 8340, (Human) 8342, (Human) 8341, (Human) 8348, (Human) 8349, (Human) 440689, (Human) 128312, (Fruit fly) 326273, (Fruit fly) 3772265, (Fruit fly) 3772081, (Fruit fly) 3771809, (Fruit fly) 3772264, (Fruit fly) 3772083, (Fruit fly) 3771891, (Fruit fly) 3772496, (Fruit fly) 3772276, (Fruit fly) 3772575, (Fruit fly) 3772271, (Fruit fly) 3772104, (Fruit fly) 3772336, (Fruit fly) 3772166, (Fruit fly) 3772502, (Fruit fly) 3771957, (Fruit fly) 3772248, (Fruit fly) 3772094, (Fruit fly) 3772058, (Fruit fly) 3772203, (Fruit fly) 3772013, (Fruit fly) 3772099, (Fruit fly) 3772299, (Mouse) 319177, (Rat) 24829, (Mouse) 319178, (Mouse) 68024, (Rat) 361247, (Mouse) 319181, (Mouse) 319182, (Mouse) 319183, (Rat) 680312, (Mouse) 319184, (Mouse) 319186, (Rat) 291157, (Mouse) 319190, (Rat) 691488, (Zebrafish) 100329290, (Zebrafish) 100329560, (Zebrafish) 100334559, (Zebrafish) 100334869, (Human) 102724334, (Dog) 478743, (Dog) 482202, (Dog) 608682, (Zebrafish) 100333496, (Zebrafish) 386920
chromatin assembly or disassembly
innate immune response in mucosa
nucleosome assembly
antibacterial humoral response
defense response to Gram-positive bacterium
chromatin organization
nucleosome disassembly
inflammatory response
plasminogen activation
spermatogenesis, exchange of chromosomal proteins
positive regulation of binding
cellular response to acid chemical
mononuclear cell migration
biological_process
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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