Active Motif

Histone H3K9ac Monoclonal Antibody (1B10)

Histone H3K9ac Antibody in Immunocytochemistry (ICC/IF)

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Histone H3K9ac Antibody (61251) in ICC/IF

Histone H3 acetyl Lys9 antibody tested by immunofluorescence. Detection of Histone H3 acetyl Lys9 by immunofluorescence. HeLa cells were stained with Histone H3 acetyl Lys9 antibody at a dilution of 2 µg/mL. Top panel: DAPI. Middle panel: Histone H3 acetyl Lys9 antibody staining. Bottom panel: merge.

Histone H3K9ac Antibody in Western Blot (WB)

Histone H3K9ac Antibody in ChIP Assay (ChIP)

Histone H3K9ac Antibody in ChIP-Sequencing (ChIP-Seq)

Histone H3K9ac Antibody in Dot Blot (DB)

Product Image Gallery

Product Details

61251

Applications

Tested Dilution

Publications

Western Blot (WB)

0.2-2 µg/mL

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

Assay-dependent

View 1 publication 1 publication

ChIP assay (ChIP)

5 µg

View 2 publications 2 publications

ChIP-sequencing (ChIP-Seq)

2-5 µg

View 2 publications 2 publications

Dot blot (DB)

Assay-dependent

Miscellaneous PubMed (MISC)

View 3 publications 3 publications

Product Specifications

Species Reactivity

Human, Mouse

Published species

Fruit fly, Human, Mouse

Host/Isotype

Mouse / IgG3

Class

Monoclonal

Type

Antibody

Clone

1B10

Immunogen

The Histone H3 acetyl Lys9 antibody was raised against a peptide including acetyl-lysine 9 of human Histone H3.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

63mM sodium citrate/70mM sodium phosphate, pH 8, with 30% glycerol, 301mM NaCl

Contains

0.035% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2793569

Target Information

Histone H3 is one of the DNA-binding proteins found in the chromatin of all eukaryotic cells. H3 along with four core histone proteins binds to DNA forming the structure of the nucleosome. Histones play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. Post translationally, histones are modified in a variety of ways to either directly change the chromatin structure or allow for the binding of specific transcription factors. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of post-translational modification that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Deciphering the molecular effects of romidepsin on germ cell tumours: DHRS2 is involved in cell cycle arrest but not apoptosis or induction of romidepsin effectors.

Journal of cellular and molecular medicine

Nettersheim D,Berger D,Jostes S,Skowron M,Schorle H

Research topic: Testicular cancer

Tue Jan 01 00:00:00 UTC 2019

Histone variants H2A.Z and H3.3 coordinately regulate PRC2-dependent H3K27me3 deposition and gene expression regulation in mES cells.

BMC biology

Wang Y,Long H,Yu J,Dong L,Wassef M,Zhuo B,Li X,Zhao J,Wang M,Liu C,Wen Z,Chang L,Chen P,Wang QF,Xu X,Margueron R,Li G

Research topic: Gene Regulation

Mon Sep 24 00:00:00 UTC 2018

XPC is an RNA polymerase II cofactor recruiting ATAC to promoters by interacting with E2F1.

Nature communications

Bidon B,Iltis I,Semer M,Nagy Z,Larnicol A,Cribier A,Benkirane M,Coin F,Egly JM,Le May N

Research topic: DNA Repair

Wed Jul 04 00:00:00 UTC 2018

Pre-treatment with amitriptyline causes epigenetic up-regulation of neuroprotection-associated genes and has anti-apoptotic effects in mouse neuronal cells.

Neurotoxicology and teratology

Tran NQV,Nguyen AN,Takabe K,Yamagata Z,Miyake K

Research topic: Aging - Neurodegenerative Disease

Sat Jul 01 00:00:00 UTC 2017

Using Chromatin Immunoprecipitation in Toxicology: A Step-by-Step Guide to Increasing Efficiency, Reducing Variability, and Expanding Applications.

Current protocols in toxicology

McCullough SD,On DM,Bowers EC

Research topic: Methods Paper

Tue May 02 00:00:00 UTC 2017

The Rb1 tumour suppressor gene modifies telomeric chromatin architecture by regulating TERRA expression.

Scientific reports

Gonzalez-Vasconcellos I,Schneider R,Anastasov N,Alonso-Rodriguez S,Sanli-Bonazzi B,Fernández JL,Atkinson MJ

Research topic: Osteosarcoma, Telomeres

Tue Feb 07 00:00:00 UTC 2017

Epigenetic Repeat-Induced Gene Silencing in the Chromosomal and Extrachromosomal Contexts in Human Cells.

PloS one

Mitsuda SH,Shimizu N

Research topic: Gene Regulation

Mon Jul 31 00:00:00 UTC 2017

Histone deacetylase inhibition promotes fibroblast apoptosis and ameliorates pulmonary fibrosis in mice.

The European respiratory journal

Sanders YY,Hagood JS,Liu H,Zhang W,Ambalavanan N,Thannickal VJ

Research topic: Idiopathic pulmonary fibrosis

Thu May 01 00:00:00 UTC 2014

Histone deacetylase inhibition promotes fibroblast apoptosis and ameliorates pulmonary fibrosis in mice.

The European respiratory journal

Sanders YY,Hagood JS,Liu H,Zhang W,Ambalavanan N,Thannickal VJ

Research topic: Idiopathic pulmonary fibrosis

Thu May 01 00:00:00 UTC 2014

Bioinformatics

Protein Aliases: Acetyl H3; Acetyl Histone H3; Acetyl-H3 K9; Acetyl-H3 Lys9; Acetyl-Histone H3 K9; Acetyl-Histone H3 Lys9; H3 histone; H3 histone family, member A; H3 histone family, member I; H3 histone family, member J; H3 histone family, member K; H3 histone family, member L; H3 histone family, member M; H3 histone, family 2; H3 K9ac; H3-clustered histone 13; H3-clustered histone 14; H3-clustered histone 15; H3-clustered histone 2; H3-clustered histone 3; H3-clustered histone 4; H3-clustered histone 6; H3-clustered histone 7; H3/b; H3/d; H3/f; H3/j; H3K9; H3Lys9ac; histone 1, H3a; histone 1, H3b; histone 1, H3f; histone 1, H3h; histone 1, H3j; histone 2, H3a; histone 2, H3c; histone 2, H3c2; histone 2, H3ca2; Histone 3; histone cluster 2, H3c2, pseudogene; histone gene complex 1; Histone H3; Histone H3.1; Histone H3.2; Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/i; Histone H3/j; Histone H3/k; Histone H3/l; Histone H3/m; Histone H3/o; Histone H3K9; Histone H3K9ac

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Gene Aliases: H3; H3-143; H3-291; H3-53; H3-614; H3-B; H3-F; H3.1-221; H3.1-291; H3.1-I; H3.2; H3.2-221; H3.2-614; H3.2-615; H3.2-616; H3/A; H3/i; H3/j; H3/k; H3/l; H3/M; H3/n; H3/o; H3a; H3b; H3C1; H3C10; H3C11; H3C12; H3C13; H3C14; H3C15; H3C2; H3C3; H3C4; H3C6; H3C7; H3C8; H3f; H3F1K; H3F2; H3FA; H3FB; H3FC HIST1H3C; H3FD; H3FF; H3FH; H3FI; H3FJ; H3FK; H3FL; H3FM; H3FN; H3g; H3h; H3i; Hist1; HIST1H3A; HIST1H3B; Hist1h3c; HIST1H3D; HIST1H3E; HIST1H3F; HIST1H3G; HIST1H3H; HIST1H3I; HIST1H3J; HIST2H3A; Hist2h3b; HIST2H3C; Hist2h3c1; Hist2h3c2; Hist2h3c2-ps; Hist2h3ca1; Hist2h3ca2; HIST2H3D; M32461

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UniProt ID: (Human) P68431, (Human) Q71DI3, (Mouse) P68433, (Mouse) P84228, (Mouse) A3KMN6

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Entrez Gene ID: (Human) 8350, (Human) 8358, (Human) 8968, (Human) 8357, (Human) 8356, (Human) 333932, (Human) 126961, (Mouse) 360198, (Mouse) 319150, (Mouse) 260423, (Mouse) 319152, (Mouse) 97114

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Function(s)

DNA binding protein binding histone binding protein heterodimerization activity cadherin binding involved in cell-cell adhesion

Process(es)

chromatin silencing at rDNA nucleosome assembly blood coagulation gene silencing by RNA cellular protein metabolic process negative regulation of gene expression, epigenetic positive regulation of gene expression, epigenetic positive regulation of defense response to virus by host DNA replication-dependent nucleosome assembly protein heterotetramerization regulation of gene silencing telomere organization cell-cell adhesion xenophagy

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Histone H3K9ac Monoclonal Antibody (1B10)

Histone H3K9ac Antibody (61251) in ICC/IF

Product Details

61251

Western Blot (WB)

Immunocytochemistry (ICC/IF)

ChIP assay (ChIP)

ChIP-sequencing (ChIP-Seq)

Dot blot (DB)

Miscellaneous PubMed (MISC)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

Bioinformatics

Performance Guarantee

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