Active Motif

Histone H4K16ac Polyclonal Antibody

Histone H4K16ac Antibody in Western Blot (WB)

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Histone H4K16ac Antibody (39167) in WB

Histone H4K16ac antibody (pAb) tested by Western blot. HeLa nuclear extract (20 µg) probed with Histone H4 acetyl Lys16 polyclonal antibody (1:1,000 dilution). Lane 1: No treatment. Lane 2: Cells treated with sodium butyrate.

Histone H4K16ac Antibody in ChIP Assay (ChIP)

Histone H4K16ac Antibody in ChIP-Sequencing (ChIP-Seq)

Histone H4K16ac Antibody in ELISA (ELISA)

Histone H4K16ac Antibody in Dot Blot (DB)

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Product Details

39167

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:5,000

View 2 publications 2 publications

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

ELISA (ELISA)

Assay-dependent

ChIP assay (ChIP)

10 µL

View 1 publication 1 publication

ChIP-sequencing (ChIP-Seq)

10 µL

View 4 publications 4 publications

Dot blot (DB)

Assay-dependent

Miscellaneous PubMed (MISC)

View 4 publications 4 publications

Product Specifications

Species Reactivity

Fruit fly, Human, Mouse, Yeast

Published species

Fruit fly, Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

This Histone H4 acetyl Lys16 antibody was raised against a peptide including acetyl-lysine 16 of histone H4.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

whole serum with 30% glycerol

Contains

0.035% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

AB_2636968

Target Information

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Deciphering the molecular effects of romidepsin on germ cell tumours: DHRS2 is involved in cell cycle arrest but not apoptosis or induction of romidepsin effectors.

Journal of cellular and molecular medicine

Nettersheim D,Berger D,Jostes S,Skowron M,Schorle H

Research topic: Testicular cancer

Tue Jan 01 00:00:00 UTC 2019

Facultative dosage compensation of developmental genes on autosomes in Drosophila and mouse embryonic stem cells.

Nature communications

Valsecchi CIK,Basilicata MF,Semplicio G,Georgiev P,Gutierrez NM,Akhtar A

Research topic: Gene Regulation

Fri Sep 07 00:00:00 UTC 2018

Epigenetic dysregulation of TET2 in human glioblastoma.

Oncotarget

García MG,Carella A,Urdinguio RG,Bayón GF,Lopez V,Tejedor JR,Sierra MI,García-Toraño E,Santamarina P,Perez RF,Mangas C,Astudillo A,Corte-Torres MD,Sáenz-de-Santa-María I,Chiara MD,Fernández AF,Fraga MF

Research topic: Brain & Nerves - Glioblastoma

Fri May 25 00:00:00 UTC 2018

The sex of specific neurons controls female body growth in Drosophila.

PLoS biology

Sawala A,Gould AP

Research topic: Brain & Nerves

Sun Oct 01 00:00:00 UTC 2017

The Rb1 tumour suppressor gene modifies telomeric chromatin architecture by regulating TERRA expression.

Scientific reports

Gonzalez-Vasconcellos I,Schneider R,Anastasov N,Alonso-Rodriguez S,Sanli-Bonazzi B,Fernández JL,Atkinson MJ

Research topic: Osteosarcoma, Telomeres

Tue Feb 07 00:00:00 UTC 2017

LSD1n is an H4K20 demethylase regulating memory formation via transcriptional elongation control.

Nature neuroscience

Wang J,Telese F,Tan Y,Li W,Jin C,He X,Basnet H,Ma Q,Merkurjev D,Zhu X,Liu Z,Zhang J,Ohgi K,Taylor H,White RR,Tazearslan C,Suh Y,Macfarlan TS,Pfaff SL,Rosenfeld MG

Research topic: Brain & Nerves

Tue Sep 01 00:00:00 UTC 2015

BET protein Brd4 activates transcription in neurons and BET inhibitor Jq1 blocks memory in mice.

Nature neuroscience

Korb E,Herre M,Zucker-Scharff I,Darnell RB,Allis CD

Research topic: Neurobiology

Thu Oct 01 00:00:00 UTC 2015

BET protein Brd4 activates transcription in neurons and BET inhibitor Jq1 blocks memory in mice.

Nature neuroscience

Korb E,Herre M,Zucker-Scharff I,Darnell RB,Allis CD

Research topic: Neurobiology

Thu Oct 01 00:00:00 UTC 2015

Histone modifications change with age, dietary restriction and rapamycin treatment in mouse brain.

Oncotarget

Gong H,Qian H,Ertl R,Astle CM,Wang GG,Harrison DE,Xu X

Research topic: Neurodegeneration, Alzheimers, Parkinson, Huntington

Tue Jun 30 00:00:00 UTC 2015

Analysis of model replication origins in Drosophila reveals new aspects of the chromatin landscape and its relationship to origin activity and the prereplicative complex.

Molecular biology of the cell

Liu J,McConnell K,Dixon M,Calvi BR

Research topic: DNA Replication

Sun Jan 01 00:00:00 UTC 2012

Role of histone acetylation in the stimulatory effect of valproic acid on vascular endothelial tissue-type plasminogen activator expression.

PloS one

Larsson P,Ulfhammer E,Magnusson M,Bergh N,Lunke S,El-Osta A,Medcalf RL,Svensson PA,Karlsson L,Jern S

Research topic: Cardiovascular

Fri Jun 29 00:00:00 UTC 2012

Loss of the methyl lysine effector protein PHF20 impacts the expression of genes regulated by the lysine acetyltransferase MOF.

The Journal of biological chemistry

Badeaux AI,Yang Y,Cardenas K,Vemulapalli V,Chen K,Kusewitt D,Richie E,Li W,Bedford MT

Research topic: Transcription Regulation

Mon Jan 02 00:00:00 UTC 2012

Bioinformatics

Protein Aliases: acetyl Histone H4; Acetyl-H4 K16; Acetyl-H4 Lys16; Acetyl-Histone H4 K16; Acetyl-Histone H4 Lys16; CG3379-PA; CG3379-PB; CG3379-PC; CG3379-PD; H4 histone family, member A; H4 histone family, member E; H4 histone family, member I; H4 histone family, member J; H4 histone family, member M; H4 histone family, member N; H4 histone, family 2; H4 K16ac; H4K16; H4K16a; H4Lys16ac; His4r-PA; His4r-PB; His4r-PC; His4r-PD; HIST1H4; histone 1, H4a; histone 1, H4b; histone 1, H4e; histone 1, H4i; histone 1, H4j; histone 2, H4; histone 2, H4a; Histone 4; Histone 4 family, member M; histone 4, H4; Histone Cluster 4; histone family member; Histone H4; Histone H4 acetyl; histone H4 replacement; Histone H4A; Histone H4K16; Histone H4K16ac; histone IV, family 2

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Gene Aliases: BcDNA:RH52884; CG31611; CG3379; CG33869; CG33871; CG33873; CG33875; CG33877; CG33879; CG33881; CG33883; CG33885; CG33887; CG33889; CG33891; CG33893; CG33895; CG33897; CG33899; CG33901; CG33903; CG33905; CG33907; CG33909; dJ160A22.2; Dmel\CG3379; Dmel_CG3379; FBtr0082962; FO108; H4; H4-12; H4-16; H4-53; H4/A; H4/B; H4/C; H4/D; H4/E; H4/G; H4/H; H4/I; H4/J; H4/K; H4/M; H4/N; H4/O; H4/p; H4C1; H4C11; H4C12; H4C13; H4C14; H4C15; H4C16; H4C2; H4C3; H4C4; H4C5; H4C6; H4C8; H4C9; H4f16; H4F2; H4F2iv; H4FA; H4FB; H4FC; H4FD; H4FE; H4FG; H4FH; H4FI; H4FJ; H4FK; H4FM; H4FN; H4FO; H4M; H4r; His4; His4-88CD; His4:CG31611; His4:CG33869; His4:CG33871; His4:CG33873; His4:CG33875; His4:CG33877; His4:CG33879; His4:CG33881; His4:CG33883; His4:CG33885; His4:CG33887; His4:CG33889; His4:CG33891; His4:CG33893; His4:CG33895; His4:CG33897; His4:CG33899; His4:CG33901; His4:CG33903; His4:CG33905; His4:CG33907; His4:CG33909; His4R; HIST1H4A; HIST1H4B; HIST1H4C; HIST1H4D; HIST1H4E; HIST1H4F; HIST1H4H; HIST1H4I; HIST1H4J; HIST1H4K; HIST1H4L; Hist1h4m; HIST2H4; HIST2H4A; HIST2H4B; HIST4H4; X04652

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UniProt ID: (Human) P62805, (Fruit fly) P84040, (Mouse) P62806

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Entrez Gene ID: (Human) 8359, (Human) 8366, (Human) 8367, (Human) 8294, (Human) 8363, (Human) 8370, (Human) 121504, (Fruit fly) 41773, (Mouse) 326619, (Mouse) 326620, (Mouse) 319158, (Mouse) 97122

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Function(s)

DNA binding protein binding protein domain specific binding histone binding poly(A) RNA binding protein heterodimerization activity

Process(es)

chromatin silencing at rDNA double-strand break repair via nonhomologous end joining nucleosome assembly DNA replication-dependent nucleosome assembly DNA replication-independent nucleosome assembly DNA-templated transcription, initiation telomere capping gene silencing by RNA telomere organization CENP-A containing nucleosome assembly cellular protein metabolic process negative regulation of megakaryocyte differentiation negative regulation of gene expression, epigenetic positive regulation of gene expression, epigenetic protein heterotetramerization beta-catenin-TCF complex assembly chromatin assembly or disassembly centrosome duplication

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Histone H4K16ac Polyclonal Antibody

Histone H4K16ac Antibody (39167) in WB

Product Details

39167

Western Blot (WB)

Immunocytochemistry (ICC/IF)

ELISA (ELISA)

ChIP assay (ChIP)

ChIP-sequencing (ChIP-Seq)

Dot blot (DB)

Miscellaneous PubMed (MISC)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

Bioinformatics

Performance Guarantee

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