Invitrogen

Histone Macro-H2A.1 Recombinant Rabbit Monoclonal Antibody (JU22-83)

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Datasheet
Protocols
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Datasheet
Protocols
Questions & Answers

Product Details

MA5-32803

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:2,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200
-

Immunocytochemistry (ICC/IF)

1:50-1:200
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

JU22-83

Immunogen

Synthetic peptide within Human macroH2A1 aa 232-281

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

TBS, pH 7.4, with 40% Glycerol, 0.05% BSA

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2810079

Product Specific Information

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

Target Information

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Core histone macro-H2A.1; H2A histone family, member Y; H2A.y; H2A/y; Histone H2A.y; Histone macroH2A1; histone macroH2A1.1; histone macroH2A1.2; MACROH2A1.2; Medulloblastoma antigen MU-MB-50.205; mH2A1

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Gene Aliases: H2A.y; H2A/y; H2AF12M; H2AFY; MACROH2A1; MACROH2A1.1; macroH2A1.2; mH2A1

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UniProt ID: (Human) O75367, (Rat) Q02874, (Mouse) Q9QZQ8

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Entrez Gene ID: (Human) 9555, (Rat) 29384, (Mouse) 26914

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Function(s)
rDNA binding RNA polymerase II regulatory region sequence-specific DNA binding RNA polymerase II core promoter sequence-specific DNA binding core promoter sequence-specific DNA binding DNA binding protein binding double-stranded methylated DNA binding enzyme binding protein kinase binding protein serine/threonine kinase inhibitor activity chromatin DNA binding nucleosomal DNA binding transcription regulatory region DNA binding protein heterodimerization activity molecular_function chromatin binding
Process(es)
negative regulation of transcription from RNA polymerase II promoter regulation of cell growth nucleosome assembly chromatin silencing dosage compensation covalent chromatin modification regulation of lipid metabolic process negative regulation of histone phosphorylation positive regulation of maintenance of mitotic sister chromatid cohesion regulation of gene expression, epigenetic positive regulation of keratinocyte differentiation negative regulation of gene expression, epigenetic positive regulation of gene expression, epigenetic negative regulation of histone H3-K4 methylation negative regulation of histone H3-K27 methylation regulation of chromatin silencing at rDNA establishment of protein localization to chromatin negative regulation of protein serine/threonine kinase activity negative regulation of transcription of nuclear large rRNA transcript from RNA polymerase I promoter negative regulation of cell cycle G2/M phase transition negative regulation of protein localization to chromosome, telomeric region biological_process chromatin modification
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