Hamburger Menu Button
Thermo Fisher Scientific Logo
Sign in
Don't have an account ? Create Account
  • Products
    • Antibodies
    • Cell Culture Media
    • Chemicals
    • Chromatography Columns and Cartridges
    • Lab Equipment
    • Lab Plasticware and Supplies
    • Microplates
    • Oligos, Primers, Probes and Genes
    • TaqMan Real-Time PCR Assays
    • Greener Products
    • See all product categories
  • Applications
    • Bioprocessing
    • Cell Culture and Transfection
    • Cell and Gene Therapy
    • Chromatography
    • Molecular Testing
    • Digital Solutions
    • DNA and RNA Extraction and Analysis
    • Spectroscopy, Elemental and Isotope Analysis
    • See all applications and techniques
  • Services
    • 360° CDMO and CRO Solutions
    • CDMO Services
    • CRO Services
    • Custom Services
    • Enterprise Services
    • Financial and Leasing Services
    • Instrument Services
    • Lab Informatics
    • OEM and Commercial Supply
    • Training Services
    • Unity Lab Services
    • See all services
  • Help and Support
    • How to Order
    • Instrument Support
    • Learning Centers
    • Register for an Account
    • Technical Support Centers
    • See all help and support topics
  • Popular
    • TaqMan Real-Time PCR Assays
      TaqMan Real-Time PCR Assays
    • Antibodies
      Antibodies
    • Oligos, Primers & Probes
      Oligos, Primers & Probes
    • GeneArt Gene Synthesis
      GeneArt Gene Synthesis
    • Cell Culture Plastics
      Cell Culture Plastics
  • Contact Us
  • Quick Order
  • Order Status and Tracking
  • Documents and Certificates
Thermo Fisher Scientific Logo

Search Thermo Fisher Scientific

Search All
Search button Close
          • Order Status
          • Quick Order
          • Sign in
            Sign in
            Don't have an account ? Create Account
            • Account
            • Check Order Status
            • Aspire Member Program
            • Connect: Lab, Data, Apps
            • Custom Products & Projects
            • Services Central

          Disclaimer

          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • HuC/HuD Antibodies

          Invitrogen

          HuC/HuD Monoclonal Antibody (16A11), Biotin

          33 Published Figures
          63 References
          View all (2) HuC/HuD antibodies

          Promotion

          View available promotion(s)

          Promo code: {{promo.promoCode}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}

          Datasheet
          Protocols
          Questions & Answers
          Datasheet
          Protocols
          Questions & Answers

          Cite HuC/HuD Monoclonal Antibody (16A11), Biotin

          • Antibody Testing Data (1)
          • Published Figures (33)
          HuC/HuD Antibody in Immunohistochemistry (IHC)
          Group 53 Created with Sketch.
          HuC/HuD Antibody in Immunohistochemistry (IHC)
          Group 53 Created with Sketch.

          FIGURE: 1 / 34

          {{ $ctrl.currentElement.advancedVerification.fullName }}
          {{ $ctrl.currentElement.advancedVerification.text }}

          HuC/HuD Antibody (A-21272) in IHC

          Mouse brain sections were labeled with the NeuroTrace® 500/525 green-fluorescent Nissl stain (Product # N-21480) or with our biotin-XX conjugate of anti-HuC/HuD antibody, followed by visualization with Alexa Fluor® 568 streptavidin (Product # S-11226). Both sections were counterstained with DAPI (Product # D1306, D3571, D21490) (Product # A-21272) {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
          Published figure supplied by benchsci-logo
          PMID: {{$ctrl.currentElement.benchSciPubmedId}}
          View Product

          {{$ctrl.videoDesc}}

          {{$ctrl.videoLongDesc}}

          HuC/HuD Antibody in Immunohistochemistry (IHC)
          HuC/HuD Antibody in Immunohistochemistry (IHC)
          HuC/HuD Antibody in Immunohistochemistry (IHC)
          HuC/HuD Antibody in Immunohistochemistry (IHC)
          HuC/HuD Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC)
          HuC/HuD Antibody in Immunocytochemistry (ICC/IF)
          HuC/HuD Antibody in Immunocytochemistry (ICC/IF)
          HuC/HuD Antibody in Immunocytochemistry (ICC/IF)
          HuC/HuD Antibody in Immunocytochemistry (ICC/IF)
          HuC/HuD Antibody in Immunocytochemistry (ICC/IF)
          HuC/HuD Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC)
          HuC/HuD Antibody in Immunohistochemistry, Immunohistochemistry (PFA fixed) (IHC, IHC (PFA))
          HuC/HuD Antibody in Immunohistochemistry, Immunohistochemistry (PFA fixed) (IHC, IHC (PFA))
          HuC/HuD Antibody in Immunohistochemistry, Immunohistochemistry (PFA fixed) (IHC, IHC (PFA))
          HuC/HuD Antibody in Immunohistochemistry (IHC)
          HuC/HuD Antibody in Immunohistochemistry (IHC)
          HuC/HuD Antibody in Immunohistochemistry (IHC)
          HuC/HuD Antibody in Immunohistochemistry (IHC)
          HuC/HuD Antibody in Immunohistochemistry (IHC)
          HuC/HuD Antibody in Immunohistochemistry (IHC)
          HuC/HuD Antibody in Immunohistochemistry (IHC)
          HuC/HuD Antibody in Immunohistochemistry (IHC)
          HuC/HuD Antibody in Immunohistochemistry (IHC)
          HuC/HuD Antibody in Immunohistochemistry (IHC)
          HuC/HuD Antibody in Immunohistochemistry (IHC)
          HuC/HuD Antibody in Immunohistochemistry (IHC)
          HuC/HuD Antibody in Immunohistochemistry (IHC)
          HuC/HuD Antibody in Immunohistochemistry (IHC)
          HuC/HuD Antibody in Immunohistochemistry (IHC)
          HuC/HuD Antibody in Immunohistochemistry (IHC)
          HuC/HuD Antibody in Immunohistochemistry (IHC)
          HuC/HuD Antibody in Immunohistochemistry (IHC)
          HuC/HuD Antibody in In Situ Hybridization (ISH)
          HuC/HuD Antibody in In Situ Hybridization (ISH)

          Product Details

          A-21272

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1-5 µg/mL
          -

          Immunohistochemistry (IHC)

          5-20 µg/mL
          View 38 publications 38 publications

          Immunohistochemistry (Paraffin) (IHC (P))

          -
          View 1 publication 1 publication

          Immunohistochemistry (PFA fixed) (IHC (PFA))

          -
          View 2 publications 2 publications

          Immunohistochemistry (Frozen) (IHC (F))

          -
          View 4 publications 4 publications

          Immunocytochemistry (ICC/IF)

          -
          View 9 publications 9 publications

          ELISA (ELISA)

          1-10 µg/mL
          -

          In Situ Hybridization (ISH) (ISH)

          -
          View 1 publication 1 publication

          Miscellaneous PubMed (MISC)

          -
          View 8 publications 8 publications
          Product Specifications

          Species Reactivity

          Human

          Published species

          Fish, Guinea pig, Human, Mouse, Pig, Rat, Zebrafish

          Host/Isotype

          Mouse / IgG2b, kappa

          Class

          Monoclonal

          Type

          Antibody

          Clone

          16A11

          Immunogen

          Human HuC/HuD neuronal protein.

          Conjugate

          Biotin Biotin Biotin
          • Unconjugated
          • Request custom conjugation

          Form

          Lyophilized

          Purification

          purified

          Storage buffer

          PBS

          Contains

          no preservative

          Storage conditions

          -20°C

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2535822

          Product Specific Information

          This antibody recognizes the Elav family members HuC, HuD and Hel-N1 neuronal proteins. It does not recognize HuR, another Elav family member that is present in all proliferating cells. The antibody has been shown to specifically label neuronal cells in zebrafish, chick, canaries, and humans, and is likely to label neuronal cells in most vertebrate species. Labeling is visible early in development, at about the time that the neurons leave the mitotic cycle.

          Storage and reconstitution: Upon receipt, lyophilized antibodies should be stored desiccated at -20ºC or lower. When properly stored, these products are stable for at least one year. To prepare stock solution, reconstitute the antibody in 0.5-1 mL PBS, pH 7.4, containing 1% BSA. Store the solution for up to two weeks at 4ºC with the addition of 2mM sodium azide. For longer storage, divide solutions into single-use aliquots and freeze at -20ºC, avoidig freeze/thaw cycles.

          For best results in immunohistochemistry, antigen retrieval is required. Use 50mM Tris, pH 8.0, for 30 minutes. The biotin-XX labeled antibody can be detected by using fluorescently labeled avidin, streptavidin or NeutrAvidin conjugates.

          Target Information

          Anti-Hu antibodies were originally isolated from a patient with paraneoplastic encephalomyelitis, a rare condition of selective neural tissue injury associated with small cell lung carcinoma. The antibodies bind specifically to antigens present exclusively in neuronal cells and are thus useful as markers of neuronal cells in tissue. The Hu antigen is an RNA-binding protein of the embryonic lethal abnormal visual (Elav) family.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: ELAV (embryonic lethal, abnormal vision, Drosophila)-like 3 (Hu antigen C); ELAV (embryonic lethal, abnormal vision, Drosophila)-like 4 (Hu antigen D); ELAV-like protein 3; ELAV-like protein 4; Hu antigen C; Hu antigen D; Hu-antigen C; Hu-antigen D; HuC; HuD; Paraneoplastic cerebellar degeneration-associated antigen; Paraneoplastic encephalomyelitis antigen HuD; Paraneoplastic limbic encephalitis antigen 21

          View more View less

          Gene Aliases: ELAVL3; ELAVL4; HUC; HUCL; HUD; PLE21; PNEM

          View more View less

          UniProt ID: (Human) Q14576, (Human) P26378

          View more View less

          Entrez Gene ID: (Human) 1995, (Human) 1996

          View more View less

          Function(s)
          nucleotide binding AU-rich element binding RNA binding mRNA 3'-UTR binding
          Process(es)
          RNA processing mRNA processing nervous system development cell differentiation
          It has to be done as per old AB suggested Products section.
          guarantee_icon

          Performance Guarantee

          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

          Learn more
          help_icon

          We're here to help

          Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.

          Contact tech support
          Ordering Plus Icon Minus Icon
          • Order Status
          • Order Help
          • Quick Order
          • Supply Center
          • eProcurement
          Support Plus Icon Minus Icon
          • Help and Support
          • Contact Us
          • Technical Support Centers
          • Documents and Certificates
          • Report a Site Issue
          Resources Plus Icon Minus Icon
          • Learning Centers
          • Promotions
          • Events and Webinars
          • Social Media
          About Thermo Fisher Plus Icon Minus Icon
          • About Us
          • Careers
          • Investors
          • News
          • Social Responsibility
          • Trademarks
          • Consumer Health Data Privacy Policy
          Our Portfolio Plus Icon Minus Icon
          • Thermo Scientific
          • Applied Biosystems
          • Invitrogen
          • Gibco
          • Ion Torrent
          • Fisher Scientific
          • Unity Lab Services
          • Patheon
          • PPD
          • Terms & Conditions
          • Privacy Information Center
          • Price & Freight Policy
          © 2006-2025 Thermo Fisher Scientific Inc. All rights reserved. All trademarks are the property of Thermo Fisher Scientific and its subsidiaries unless otherwise specified.
          United States flag icon
          United States

          Your items have has been added!


          Host server : magellan-search-67c77f6dc7-n6nf2:80/100.66.135.186:80.
          git-commit: 545e792480003146e539d624bd2a21b51d198228
          git-url: https://github.com/thermofisher/magellan-search
          git-branch: release/2.31.0-2025.06.23-1.0