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Human Serum Albumin Antibody (600-403-033) in WB
Western Blot of human serum albumin. Lane 1: 1:400 of FBS. Lane 2: 1:1600 of FBS. Lane 3: 1:400 of normal rabbit serum. Lane 4: 1:1600 of normal rabbit serum. Lane 5: 1:400 of normal human serum. Lane 6: 1:1600 of normal human serum. Lane 7: 1:10 of human serum albumin (10mg/mL). Lane 8: 1:40 of human serum albumin (10mg/mL). Lane 9: molecular weight. Right Blot: Secondary only incubated for control. Primary antibody: human serum albumin at 1:400 for overnight at 4°C. Secondary antibody: Biotin conjugated rabbit anti human seru... View More
Product Details
600-403-033
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Anti-Human Serum Albumin Antibody was produced by repeated immunizations with Human Serum Albumin.
Conjugate
Form
Lyophilized
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
0.02M potassium phosphate, pH 7.2, with 10mg/mL PEG, 0.15M NaCl
Contains
0.01% gentamicin sulfate
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Human Serum Albumin antibody was purified from pooled rabbit sera obtained from rabbits immunized with Human Serum Albumin. The purified antibody was then conjugated to horseradish peroxidase and subsequently purified followed by exhaustive dialysis against the buffer stated above.
Target Information
Albumin is a soluble, monomeric protein with a molecular weight of approximately 65-67 kDa that comprises about one-half of the blood serum protein. Human serum albumin (HAS) is the most abundant protein in human blood plasma, and has a serum half-life of approximately 20 days. Albumin functions primarily as a carrier protein for steroids, fatty acids, and thyroid hormones and plays a role in stabilizing extracellular fluid volume. Albumin is a globular, unglycosylated serum protein synthesized in the liver as pre-pro-albumin which has an N-terminal peptide that is removed before the nascent protein is released from the rough `produce the secreted albumin. Serum albumin has a good binding capacity for water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs. The main function of albumin is the regulation of the colloidal osmotic pressure of blood.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Albumin; albumin (32 AA); albumin (AA 34); cell growth inhibiting protein 42; DKFZp779N1935; growth-inhibiting protein 20
Gene Aliases: ALB; ANALBA; FDAH; GIG20; GIG42; PRO0883; PRO0903; PRO1341; PRO1708; PRO2044; PRO2619; PRO2675; UNQ696/PRO1341
UniProt ID: (Human) P02768
Entrez Gene ID: (Human) 213
retina homeostasis
platelet degranulation
transport
receptor-mediated endocytosis
response to nutrient
cellular response to starvation
response to organic substance
bile acid and bile salt transport
hemolysis by symbiont of host erythrocytes
lipoprotein metabolic process
negative regulation of apoptotic process
negative regulation of programmed cell death
sodium-independent organic anion transport
positive regulation of circadian sleep/wake cycle, non-REM sleep
response to mercury ion
maintenance of mitochondrion location
response to platinum ion
cellular oxidant detoxification
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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